NEB内切酶产品具体Buffer活性表.doc

1、NEBuffer Activity Chart for Restriction EnzymesNew England Biolabs provides a color-coded 10X NEBuffer with every restriction endonuclease to ensure 100% activity. To help select the best conditions for double digests, this chart shows the optimal (supplied) NEBuffer and approximate activity in the

2、four standard NEBuffers for each enzyme. If BSA is supplied with an enzyme, it is included in all NEBuffer activity reactions. In addition, the table also shows recommended reaction temperature, heat inactivation temperature and recommended diluent buffer. Please click here for additional informatio

3、n about: Unique NEBuffer Activity | Double Digestion | Heat Inactivation | Activity at 37C | Diluent Buffers | Time-Saver Enzymes | High Fidelity (HF) Restriction Enzymes This chart has been updated to reflect the change of 54 restriction enzymes to NEBuffer 4 on Nov.1, 2008.Click for the Chart Lege

4、nd, Icon Description A | B | C | D | E | F | H | I | K | M | N | P | R | S | T | X | Z | % Activity in NEBuffersEnzyme Supplied NEBuffer1 2 3 4HeatInactiva-tion CIncuba.Temp.CDiluentAatII NEBuffer 4 0 50 50 100 65 37 AAcc65I NEBuffer 3 + BSA 10 75 100 25 65 37 AAccI NEBuffer 4 50 50 10 100 80 37 AAc

5、iI NEBuffer 3 25 50 100 50 65 37 AAclI NEBuffer 4 + BSA 10 10 0 100 No 37 BAcuI NEBuffer 4 + SAM 50 100 50 100 65 37 BAfeI NEBuffer 4 25 50 25 100 65 37 AAflII NEBuffer 4 + BSA 50 100 25 100 65 37 AAflIII NEBuffer 3 + BSA 25 75 100 50 80 37 BAgeI NEBuffer 1 100 50 10 75 65 37 CAhdI NEBuffer 4 + BSA

6、25 75 0 100 65 37 AAleI NEBuffer 4 10 25 10 100 65 37 AAluI NEBuffer 4 100 100 75 100 65 37 BAlwI NEBuffer 4 50 100 10 100 65 37 AAlwNI NEBuffer 4 50 100 50 100 65 37 AApaI NEBuffer 4 + BSA 25 50 0 100 65 25 AApaLI NEBuffer 4 + BSA 100 100 10 100 No 37 AApeKI NEBuffer 3 25 75 100 50 No 75 BApoI NEBu

7、ffer 3 + BSA 10 75 100 75 80 50 AAscI NEBuffer 4 0 10 10 100 65 37 AAseI NEBuffer 3 NR 75 100 dd NR 65 37 BAsiSI NEBuffer 4 + BSA 50 100 100 100 80 37 BAvaI NEBuffer 4 10 75 10 100 80 37 AAvaII NEBuffer 4 50 75 10 100 65 37 AAvrII NEBuffer 4 100 100 50 100 80 37 BEnzyme top Supplied NEBuffer% in B1%

8、 in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentBaeGI NEBuffer 1 100 75 10 50 65 37 ABaeI NEBuffer 4 + BSA + SAM 50 100 50 100 65 25 ABamHI NEBuffer 3 + BSA 75 100 100 dd 100 No 37 ABamHI-HF NEBuffer 4 100 50 10 100 No 37 ABanI NEBuffer 4 + BSA 50 100 50 100 65 37 ABanII NEBuffer 4 100 10 50 100

9、 80 37 ABbsI NEBuffer 2 100 100 25 75 65 37 BBbvCI NEBuffer 4 50 100 10 100 80 37 ABbvI NEBuffer 2 100 100 25 75 65 37 BBccI NEBuffer 1 + BSA 100 50 10 50 65 37 ABceAI NEBuffer 3 + BSA 100 100 100 100 65 37 ABcgI NEBuffer 3 + SAM NR NR 100 dd NR 65 37 ABciVI NEBuffer 4 100 50 0 100 65 37 CBclI NEBuf

10、fer 3 50 100 100 dd 75 No 50 ABfaI NEBuffer 4 75 50 10 100 80 37 BBfuAI NEBuffer 3 0 75 100 10 65 50 BBfuCI NEBuffer 4 + BSA 100 50 25 100 80 37 BBglI NEBuffer 3 50 75 100 50 65 37 BBglII NEBuffer 3 10 75 100 10 No 37 ABlpI NEBuffer 4 50 100 10 100 No 37 ABmgBI NEBuffer 3 + BSA 0 25 100 10 65 37 CBm

11、rI NEBuffer 2 75 100 75 100 65 37 BBmtI NEBuffer 2 25 100 25 50 65 37 BBpmI NEBuffer 3 + BSA 75 100 100 100 65 37 BBpu10I NEBuffer 3 10 25 100 25 80 37 BBpuEI NEBuffer 4 + SAM 50 100 10 100 65 37 BBsaAI NEBuffer 4 100 100 100 100 No 37 CBsaBI NEBuffer 4 50 100 75 100 80 60 BBsaHI NEBuffer 4 + BSA 50

12、 100 100 100 80 37 ABsaI NEBuffer 4 75 75 100 100 65 50 BBsaJI NEBuffer 4 100 100 100 100 80 60 ABsaWI NEBuffer 4 + BSA 50 100 50 100 80 60 ABsaXI NEBuffer 4 75 100 10 100 No 37 BBseRI NEBuffer 4 100 100 75 100 65 37 ABseYI NEBuffer 3 10 50 100 50 65 37 BBsgI NEBuffer 4 + SAM 50 75 50 100 65 37 BBsi

13、EI NEBuffer 4 + BSA 50 100 10 100 80 60 ABsiHKAI NEBuffer 4 + BSA 50 100 100 100 No 65 BBsiWI NEBuffer 3 100 100 100 25 80 55 ABslI NEBuffer 3 10 50 100 75 80 55 ABsmAI NEBuffer 4 50 100 100 100 80 55 BBsmBI NEBuffer 3 75 100 100 100 80 55 BBsmFI NEBuffer 4 + BSA 25 50 50 100 80 65 ABsmI NEBuffer 4

14、75 100 75 100 80 65 ABsoBI NEBuffer 2 10 100 100 50 No 37 ABsp1286I NEBuffer 4 + BSA 25 25 25 100 65 37 ABspCNI NEBuffer 4 + BSA + SAM 100 75 10 100 80 25 ABspDI NEBuffer 4 25 75 50 100 65 37 ABspEI NEBuffer 3 0 10 100 0 80 37 BBspHI NEBuffer 4 10 100 50 100 65 37 ABspMI NEBuffer 3 NR NR 100 NR 65 3

15、7 BBspQI NEBuffer 4 10 50 100 100 80 50 BBsrBI NEBuffer 4 50 100 100 100 80 37 ABsrDI NEBuffer 2 + BSA 50 100 50 75 80 65 ABsrFI NEBuffer 4 10 100 100 100 No 37 CBsrGI NEBuffer 2 + BSA 25 100 10 100 80 37 ABsrI NEBuffer 3 0 50 100 10 80 65 BBssHII NEBuffer 3 100 100 100 dd 100 80 50 ABssKI NEBuffer

16、3 + BSA 0 50 100 50 80 60 ABssSI NEBuffer 3 + BSA 0 50 100 dd 10 80 37 BBstAPI NEBuffer 4 + BSA 25 100 100 100 80 60 ABstBI NEBuffer 4 75 50 25 100 No 65 ABstEII NEBuffer 3 + BSA 50 75 100 75 No 60 ABstNI NEBuffer 2 + BSA 10 100 100 75 No 60 ABstUI NEBuffer 4 100 100 50 100 No 60 ABstXI NEBuffer 3 0

17、 50 100 25 65 37 BBstYI NEBuffer 2 50 100 75 100 80 60 ABstZ17I NEBuffer 4 NR NR 100 100 No 37 BBsu36I NEBuffer 3 + BSA 0 25 100 0 80 37 ABtgI NEBuffer 3 + BSA 25 50 100 100 80 37 ABtgZI NEBuffer 4 + BSA 10 25 0 100 80 60 ABtsCI NEBuffer 4 50 100 50 100 No 50 BBtsI NEBuffer 4 + BSA 100 50 50 100 80

18、55 AEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentCac8I NEBuffer 4 50 75 100 100 65 37 BClaI NEBuffer 4 + BSA 10 50 50 100 65 37 ACspCI NEBuffer 4 + SAM 10 100 10 100 65 37 ACviAII NEBuffer 4 75 25 10 100 65 25 BCviKI-1 NEBuffer 4 10 50 25 100 80 37 ACviQI N

19、EBuffer 3 + BSA 75 100 100 75 No 25 BEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentDdeI NEBuffer 3 75 100 100 75 65 37 ADpnI NEBuffer 4 100 100 75 100 80 37 BDpnII NEBuffer DpnII NR NR 100 dd NR 65 37 BDraI NEBuffer 4 75 75 50 100 65 37 ADraIII NEBuffer 3 +

20、BSA 100 75 100 25 65 37 BDrdI NEBuffer 4 + BSA 25 50 10 100 65 37 AEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentEaeI NEBuffer 4 100 100 50 100 80 37 AEagI NEBuffer 3 10 25 100 10 65 37 CEagI-HF NEBuffer 4 25 50 10 100 65 37 CEarI NEBuffer 4 100 100 50 100 6

21、5 37 AEciI NEBuffer 2 + BSA 50 100 100 50 65 37 AEcoNI NEBuffer 4 100 100 75 100 65 37 AEcoO109I NEBuffer 4 + BSA 100 100 75 100 65 37 AEcoP15I NEBuffer 3 + BSA + ATP 75 100 100 100 65 37 AEcoRI NEBuffer EcoRI dd 100 100 100 100 65 37 CEcoRI-HF NEBuffer 4 10 100 0 100 65 37 CEcoRV NEBuffer 3 + BSA 5

22、0 75 100 50 80 37 AEcoRV-HF NEBuffer 4 25 100 100 100 65 37 BEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentFatI NEBuffer 2 10 100 50 50 65 55 AFauI NEBuffer 4 100 50 10 100 65 55 AFnu4HI NEBuffer 4 10 25 25 100 65 37 AFokI NEBuffer 4 100 100 75 100 65 37 AFs

23、eI NEBuffer 4 + BSA 100 75 0 100 65 37 BFspI NEBuffer 4 10 75 10 100 dd 65 37 CEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentHaeII NEBuffer 4 + BSA 75 100 50 100 80 37 AHaeIII NEBuffer 4 50 100 25 100 80 37 AHgaI NEBuffer 1 100 75 50 100 65 37 AHhaI NEBuffer

24、 4 + BSA 75 100 100 100 65 37 AHincII NEBuffer 3 + BSA 75 100 100 100 65 37 BHindIII NEBuffer 2 50 100 10 50 65 37 BHinfI NEBuffer 4 75 100 75 100 80 37 AHinP1I NEBuffer 4 100 100 100 100 65 37 AHpaI NEBuffer 4 25 50 10 100 No 37 AHpaII NEBuffer 1 100 50 10 50 65 37 AHphI NEBuffer 4 NR 75 0 100 65 3

25、7 BHpy166II NEBuffer 4 100 100 50 100 65 37 CHpy188I NEBuffer 4 50 25 10 100 65 37 AHpy188III NEBuffer 4 + BSA 100 100 10 100 65 37 AHpy99I NEBuffer 4 + BSA 10 10 0 100 65 37 AHpyAV NEBuffer 4 + BSA 100 100 25 100 65 37 BHpyCH4III NEBuffer 4 100 50 25 100 80 37 AHpyCH4IV NEBuffer 1 100 25 10 25 65 3

26、7 AHpyCH4V NEBuffer 4 50 50 25 100 65 37 BEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentI-CeuI NEBuffer 4 + BSA 10 10 0 100 65 37 BI-SceI NEBuffer I-SceI + BSA 10 50 50 50 65 37 BEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Tem

27、p.DiluentKasI NEBuffer 4 + BSA 25 100 0 100 65 37 BKpnI NEBuffer 1 + BSA 100 75 0 50 No 37 AEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentMboI NEBuffer 4 75 100 100 100 65 37 AMboII NEBuffer 4 100 100 50 100 65 37 CMfeI NEBuffer 4 75 50 10 100 65 37 AMfeI-HF

28、 NEBuffer 4 75 50 10 100 65 37 AMluI NEBuffer 3 25 75 100 50 65 37 AMlyI NEBuffer 4 + BSA 50 50 25 100 65 37 AMmeI NEBuffer 4 + SAM NR NR NR 100 dd 80 37 BMnlI NEBuffer 4 + BSA 75 100 50 100 65 37 BMscI NEBuffer 4 75 75 75 100 65 37 BMseI NEBuffer 4 + BSA 75 100 75 100 65 37 AMslI NEBuffer 4 100 100

29、 25 100 65 37 AMspA1I NEBuffer 4 + BSA 50 100 50 100 65 37 BMspI NEBuffer 4 75 100 50 100 80 37 AMwoI NEBuffer 3 10 75 100 75 No 60 BEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentNaeI NEBuffer 4 100 75 10 100 65 37 ANarI NEBuffer 4 100 75 75 100 65 37 ANb.Bb

30、vCI NEBuffer 2 50 100 10 100 80 37 ANb.BsmI NEBuffer 3 25 100 100 25 80 65 ANb.BsrDI NEBuffer 2 10 100 25 50 80 65 ANb.BtsI NEBuffer 4 + BSA 75 100 75 100 80 37 ANciI NEBuffer 4 100 25 10 100 No 37 ANcoI NEBuffer 3 100 100 100 100 65 37 ANcoI-HF NEBuffer 4 50 100 10 100 80 37 BNdeI NEBuffer 4 75 100

31、 75 100 65 37 ANgoMIV NEBuffer 4 100 50 10 100 80 37 ANheI NEBuffer 2 + BSA 100 100 10 100 65 37 CNheI-HF NEBuffer 4 + BSA 100 10 0 100 80 37 CNlaIII NEBuffer 4 + BSA 25 25 25 100 65 37 BNlaIV NEBuffer 4 + BSA 10 10 10 100 65 37 BNmeAIII NEBuffer 4 + SAM 10 10 0 100 80 37 BNotI NEBuffer 3 + BSA 0 50

32、 100 25 65 37 CNotI-HF NEBuffer 4 + BSA 25 100 25 100 65 37 ANruI NEBuffer 3 0 10 100 10 65 37 ANsiI NEBuffer 3 10 75 100 25 80 37 ANspI NEBuffer 2 + BSA 100 100 0 100 65 37 ANt.AlwI NEBuffer 2 25 100 25 50 80 37 ANt.BbvCI NEBuffer 4 50 100 10 100 80 37 ANt.BsmAI NEBuffer 4 100 50 10 100 65 37 ANt.B

33、spQI NEBuffer 3 10 75 100 25 80 50 BNt.BstNBI NEBuffer 3 0 10 100 10 80 55 ANt.CviPII NEBuffer 4 25 100 50 100 65 37 AEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentPacI NEBuffer 1 + BSA 100 75 10 100 65 37 APaeR7I NEBuffer 4 25 100 10 100 No 37 APciI NEBuffe

34、r 3 50 75 100 50 80 37 APflFI NEBuffer 4 + BSA 0 25 25 100 65 37 APflMI NEBuffer 3 + BSA 0 100 100 50 65 37 APhoI NEBuffer 3 50 50 100 75 No 75 API-PspI NEBuffer PI-PspI + BSA 0 10 10 10 No 65 BPI-SceI NEBuffer PI-SceI + BSA 0 0 0 0 65 37 BPleI NEBuffer 4 100 100 75 100 65 37 APmeI NEBuffer 4 + BSA

35、0 50 10 100 65 37 APmlI NEBuffer 1 + BSA 100 75 0 75 65 37 APpuMI NEBuffer 4 0 25 0 100 No 37 BPshAI NEBuffer 4 + BSA 50 75 10 100 65 37 APsiI NEBuffer 4 10 100 10 100 65 37 BPspGI NEBuffer 4 75 100 50 100 No 75 APspOMI NEBuffer 4 25 25 10 100 65 37 APspXI NEBuffer 4 0 100 10 100 80 37 BPstI NEBuffe

36、r 3 + BSA 75 75 100 50 80 37 CPvuI NEBuffer 3 + BSA 10 75 100 10 80 37 BPvuII NEBuffer 2 100 100 100 100 No 37 BPvuII-HF NEBuffer 4 0 25 0 100 80 37 BEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentRsaI NEBuffer 4 100 100 50 100 65 37 ARsrII NEBuffer 4 25 75 1

37、0 100 65 37 CEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentSacI NEBuffer 1 + BSA 100 50 10 100 65 37 ASacI-HF NEBuffer 4 + BSA 100 50 10 100 65 37 ASacII NEBuffer 4 25 75 10 100 65 37 ASalI NEBuffer 3 + BSA 0 0 100 0 65 37 ASalI-HF NEBuffer 4 10 100 100 100

38、65 37 ASapI NEBuffer 4 75 50 0 100 65 37 CSau3AI NEBuffer 1 + BSA 100 50 10 100 65 37 ASau96I NEBuffer 4 50 100 100 100 80 37 ASbfI NEBuffer 4 75 50 0 100 65 37 ASbfI-HF NEBuffer 4 50 25 0 100 65 37 BScaI NEBuffer 3 NR NR 100 dd NR 80 37 AScaI-HF NEBuffer 4 100 100 10 100 65 37 BScrFI NEBuffer 4 100

39、 100 100 100 65 37 ASexAI NEBuffer 4 + BSA 100 75 50 100 65 37 ASfaNI NEBuffer 3 0 75 100 25 65 37 BSfcI NEBuffer 4 + BSA 75 50 10 100 65 37 BSfiI NEBuffer 4 + BSA 0 100 10 100 No 50 CSfoI NEBuffer 4 25 100 50 100 No 37 BSgrAI NEBuffer 4 100 50 10 100 65 37 ASmaI NEBuffer 4 0 0 0 100 65 25 ASmlI NEB

40、uffer 4 + BSA 25 75 25 100 No 55 ASnaBI NEBuffer 4 + BSA 25 50 25 100 dd 80 37 ASpeI NEBuffer 4 + BSA 75 100 25 100 80 37 CSphI NEBuffer 2 100 100 50 100 65 37 BSphI-HF NEBuffer 4 50 25 10 100 65 37 BSspI NEBuffer SspI 50 100 50 50 65 37 CSspI-HF NEBuffer 4 25 100 0 100 65 37 BStuI NEBuffer 4 100 10

41、0 50 100 65 37 AStyD4I NEBuffer 2 10 100 100 100 65 37 BStyI NEBuffer 3 + BSA 25 75 100 10 65 37 ASwaI NEBuffer 3 + BSA 10 10 100 10 65 25 BEnzyme top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentTaqI NEBuffer 4 + BSA 50 75 100 100 80 65 BTfiI NEBuffer 4 100 100 100 1

42、00 No 65 CTliI NEBuffer 3 + BSA 25 25 100 10 No 75 BTseI NEBuffer 4 75 100 100 100 No 65 BTsp45I NEBuffer 1 + BSA 100 25 0 75 No 65 ATsp509I NEBuffer 1 100 100 100 NR No 65 ATspMI NEBuffer 4 50 75 50 100 No 75 BTspRI NEBuffer 4 + BSA 25 50 25 100 No 65 BTth111I NEBuffer 4 50 25 25 100 No 65 BEnzyme

43、top Supplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentXbaI NEBuffer 4 + BSA 0 100 75 100 65 37 AXcmI NEBuffer 2 10 100 50 50 65 37 CXhoI NEBuffer 4 + BSA 75 100 100 100 65 37 AXmaI NEBuffer 4 + BSA 25 50 0 100 65 37 AXmnI NEBuffer 4 + BSA 100 100 50 100 65 37 AEnzyme top S

44、upplied NEBuffer% in B1% in B2% in B3% in B4HeatInactiva.Incuba.Temp.DiluentZraI NEBuffer 4 100 25 10 100 No 37 ANote: The values listed in this table are approximate. They were obtained using each enzymes specific unit assay substrate DNA.某 些 内 切 酶 的 组 合 不 能 采 用 同 步 双 酶 切 法 ， 只 能 采 用 分 步 法 进 行 双 酶 切 。 上 表 中 这 些 组合 以 “seq”标 注 。