1、 l :2008- 08- 11 “:SE1 S “(30671244); 8$9$ “(08965505D); 81 S(C2005000220;C2007000515)Te:(1980- ), o,a ,p V,1V Yl FhTbYT: (1963- ), o,E , q,p V,p V 3 =,1V Y Fh 3 #% ?bh/ (SSH)阎爱华,王冬梅( j(Close)sVr/, uY“d y ?XVr s,yF B 4b 7b“d V “yF f /,yFyVr f , SAGEaMegacloneMPSS / 1 LqM * V1null+ysVr/ # 1 Tab.1 nul
2、l The principles andtheir comparionof different gene expression techniquesysVrsZEMethodsof differentgeneexpressiontechniques ThebasicprinciplesofdifferentgeneexpressiontechniquesAdvantageDisadvantagemRNAsA UPCRmRNA differentialdisplayPCR,DDRT-PCRPCR d / ,Oligo-dTMN(M= A,C,G;N= A,G,C,T)mRNAPolyAQ c,m
3、RNA-cDNAs0,5null 20H , 9,V d , M“s , 9by,eL, V“1 bL, c bcDNAVsscDNA-depresentationaldifferentialanalysis,RDA|mRNAsZ zZ,Q ccDNA,V M,V zZ_Z3Q,QMZh, PCR 9 “ bCz,L bmRNA1 p,T v, S M vH abyVrGeneexpressionfingerprinting,GEFP 3 S:B , F,ca?= ,i = M h, F 3 o3null , - = M M a a 0,i a 0 T 9+s bCz,L b ?sVr yby
4、Vr“ sSerialanalysisofgeneexpression,SAGE3 Oligo-dTcDNA, M db5null ,3null H , l ,is z,sY+sS oBt ? , V ? Bt M1yb7I35sY1d7dl) 7 =cDNATester,增刊 阎爱华等: 抑制性差减杂交技术 (SSH) 在植物学研究中的应用 81null3 =cDNADriver,y l/ 7 hh o,?Cc a aCa _ aDREy0v FlM1ybWang36 hh/ / l = s yVr ,YV1530 X H 181 X H,ty 38 =? W% s I Tb) yVrsZ
5、,BtYVy L 3 37#Z 0 38/y o,YVyVrssB U as0bBahn39y ) v SSH o, v Fs0 $bSavenstrand40,Sahre41,Watt42,Zhang43sYy = s,L M1Vryoa E 2$ VryVr a$ h 0 M1yVr o# = s M1yVr o,YVtT, s8C h/ L ,. 0 3M1y kJ. 3 3 ,2003,35(6):554- 560.25 null null , d, ,.hhZEl FxhM1yVr J. SY,2002,47(16):1237- 1241.26 null , y ,.H )l h o
6、y# Vr S , +null.( Populus euphratica)M1y X HJ. = jv:1 S,2004,25(2):76- 80.38 nullfv, U,kD,.Z 0 hhcDNA oyJ. j,2006,22(2):113- 116.39 null BahnSC,BaeM S,ParkY B, et al.Molecularcloningandcharacterizationof anoveltemperature-induced gene,blti2,frombarley(Hordeum vulgare L.)J.BiochimBiophysAc-ta,2001,15
7、22(2):134- 137.40 null Savenstrand,BroscheM,StridA, et al.Regulation of geneexpressionby low levels of iultraviole-t Bradiationin Pisumsativum:isolation of novel genes by suppressionsubtractivehybridizationJ.Plant Cell Physiol,2002,43(4):402-410.41 null SahrT,Voigt G,ParetzkeH G, et al.Caesium-affec
8、ted geneexpressioninArabidopsisthlianaJ.New Phytol,2005,165(3):747- 754.42 null WattD A.Aluminium-responsivegenes insugarcane:ident-ification andanalysisofexpressionunderoxidativestressJ.JExpBot,2003,54(385):1163- 1174.43 null ZhangY,Mian M A R, Chekhovskiy K, et al.DifferentialgeneexpressioninFestucaunderheatstress conditionsJ.JExp Bot,2005,56(413):897- 907.44 null V, S,l ,.B0kgDNA!ZJ.0,2005,36(7):961- 964null45 null g ,.Hq/ yVrsJ. j,2007,22(4):94- 98.增刊 阎爱华等: 抑制性差减杂交技术 (SSH) 在植物学研究中的应用 83null