1、 s 罗强1, 陈全成1, 吴瑶1, 姜苗苗2, 陈志鸿3, 张晓坤1, 4* , 陈海峰1*(1null厦门大学生物医学研究院, 福建厦门 361005;2null广东药学院中心实验室, 广东广州 510006;3null厦门归真堂生物科技有限公司, 福建厦门 361005;4null美国 Burnham研究所, 加利福尼亚州美国)K 1 null “ :“ d S . d L 0 s bZ E : v d , Sephadex LHnull20, ODS# M A m s ,Y V o s 8 k b T :V s 9 8 , k s Y 4null, 7null= s (1), 4nul
2、l, 7null= null6nullJ s ( 2), 4null, 5, 7null s ( 3), 4nullnullJ null7null s ( 4), d ( 5), d + ( 6), ( 7),+ ( 8), % ? ( 9)b : 1 4 nQ V s ,9 V S n Q s b1 o M null ; s ;s q I | null 20091112003 “ null 0 g S / 9 “ (3502Z20083005)Y T null * Z , Te:l ( 0592) 2187225 , Enullmai:l haifengxmunulledunull cn;
3、* f k , Te:l ( 0592) 2187202, Enullmai:l xzhang burnnullhamnullorgnull null S Selenarctosthibetanus Gnull Cuvier# 5 Ursus arctos Linnaeus 2 5 b S 1 s 2 ,L , + , Z , # 1null2bT S . d L 0 , b , “ , r 1, 3null4, 1 % h , 7 5 % h ,S % h , a8 S % h 5null9b 7 , s K , “ - S = s i “ d ,D , 8 9 p v 10null11b
4、s s m , 2 s ,V s 8 9 , H o , s ,i D v k b 4 s n Q V s ,9 n Q V S s b1null N k 4BrukerAvance 500 spectrometer H N b3200Qnulltrap( S ABI ) N b A ( “ Z 3 , HP20v d + T 3 , ODS, Sephadex LH null20Pharmacia g s , LCnull20AD rA M N b x 0 B 3 S / K 4 ,S C i b 0 v 3 D ? b2null 4 | s | x 250null2 g,J ? 2 4 |
5、 3Q , i 4 | A 9 4 234null8 gb | x J ?4 | 230null3 g, 2 L , , null , JnullS 0 , 2008, 33 (2): 152null17 null Omkar B Ijare, B S Somashekar, Y Jadegoud, et alnull 1H and13CNMR characterization and stereochemical assignments of bileacids in aqueous media Jnull Lipids, 2005, 40 (10): 1031null!2418! 35 1
6、8 2010 M 9 null null null null null null null null null null null null null null null null null null nullVolnull35, Issuenull 18September, 2010 18 null Bishambar Daya,l Jalpa Bhojawala, keshava R Rapole, et alnullChem icalsynthesis, structuralanalysis and decomposition ofNnullninulltroso bile acid c
7、onjugates J null BioorganicM ed Chem, 1996, 4(6): 885null 19 null ; , Z , R l null _ s 8 + 1HnullNMR + # x 1 s Jnull y s k , 1996, 6 ( 1): 618null 20 null D V W aterhous, Stephen Barnes, D D Muccio, et alnull Nuclearmagnetic resonance spectroscopy of bile acidsnull Development oftwonulldimensionalNM
8、R methods for the elucidation ofproton resonullnance assignments for five common hydroxylated bile acids, andtheir parentbile acid, 5nullnullcholanoicacid J null JLipid Res, 1985,26 ( 9): 1068null21 null d , D , null G Lobophytum spnull s J null S / v :1 S , 2009, 30 (4):325null22 null M GinaGlazier
9、, M arjorieA Bowmannull A review of the evidence forthe use of phytoestrogens as a replacement for traditionalestrogenreplacement therapy J null Archives IntM ed, 2001, 161 ( 9):1161null23 null ShaoyiCheng, NingsingShaw, KesungTsa,i etalnull The hypoglycenullmic effects of soy isoflavones on postmen
10、opausalwomen J null JW omen% sHealth, 2004, 13 ( 10): 1080nullChemical constituents of bear bileLUO Q iang1, CHEN Quancheng1, WU Yao1, JIANG M iaomiao2, CHEN Zhihong3,ZHANG X iaokun1, 4* , CHEN Haifeng1*( 1nullInstitute for Biom edicalResearch, Xiam en University, Xiam en 361005, China;2nullCentralL
11、aboratory, Guangdong Pharm aceuticalUniversity, Guangzhou 510006, China3nullXiam en Guizhentang Biotechnology Conull, nullLTDnull, X iam en 361005, China4nullBurnham Institute forM deicalResearch, California, USA )Abstractnull Objecctive: To study the chem ical constituents of bear bilenull M ethod:
12、 The compounds were isolated by repeatedcolumnHP20 macroporous adsorption resin, SephadexLHnull20, ODS and silicagel as packingmaterialsnull The structureswere identifiedon thebasis of extensive spectroscopic data analysis and by comparison of their spectraldata reportednull Result: N ine compoundsw
13、ereidentified as4null, 7nulldihydroxyisoflavone( 1), 4null, 7nulldihydroxynull6nullmethoxyisoflavone ( 2), 4null, 6, 7nulltrihydroxyisoflavone ( 3), 4nullnullmethoxynull7nullhydroxyisoflavone ( 4), tauroursodeoxycholicacid (5), taurochenodeoxycholicacid ( 6), ursodeoxycholic acid ( 7), chenodeoxycho
14、licacid ( 8), cholesterol( 9)null Conclusion: Compounds 1null4 were separated from bearbile for the first timenullKeywordsnull bearbile; chem ical constituents; isoflavonesdo:i 10. 4268/cjcmm201018123 I null , !2419! 35 18 2010 M 9 null null null null null null null null null null null null null null null null null null nullVolnull35, Issuenull 18September, 2010
