1、 4 3 J _ Vo l. 4 No. 3 2013 M 6 Journal of Food Safety and Quality Jun. , 2013 基金项目: n , 7 LOX1 1 2 min ? , LOX3 60 H , 11530 min , LOX2 5 min = b S14V , 7 J LOX A7 J. , K aQ 55 , K aQ pH6.0, K a i 8.0 mmol/L, 4) HW ?r LOXb 2.2.3 LOX的催化特性 bQ , Tappel , LOX b 1V13b n5 LOX 8 ; V B 8 , B _ 0B0V Ms0 ; s
2、0V V _ ; NV _ s i / b71 : n5 _0V 7 , HM 0$ ; s01Q , V1 , NV V ? O2M O21 , KV1$ LOX M , 3 _V , 7 LOX MM Fe3+, M 15b M , v LOX 00MQ L LOX QV _M 16, b _0V MM 0 ; 00 H8 s8WM ; V7 3r _ rbyN , V + V A LOX bM 0“ M1“10b 3 脂肪氧合酶的应用 3.1 脂肪氧合酶在食品中的应用 l x , V x , P , P 9 , 3 Y , : #Z 801 PV , V Vs0 ; _v= ;o , V
3、74 9,17,18b LOX b , 3 _V , _VYV ( - Ms , a=) , _VB a ? , TaP(a T T#7a aZ(Pa P?8, F J , Es7 $b c 11 , y7 b!P b Whitehead19 aYv , HPL # , 3-?a2-(E)- a 3-(Z)- -1-?a- a 2-(E)- -1-?b Gargouri20P , 3 9- _V , V 3 (P 3-(Z)- , J (b Marczy aLOX k HPL M8“!- , i 0.1 mol/L, 13-HPOD q 72%, - q 54% 21bMuller22 .x
4、3 13-HPOD, iI F := HPL T , !B 96%- , H .x , ! 3-(Z)- b Noordermeer 23 .aa LOX #, HPL, ! q50%- bN Kangsadan24 25VZ(4 | LOX, i 3 , 3 12-3 _V , H 3 15- 3 _V , i/ - 3-(Z)- , - b 3.2 脂肪氧合酶在植物生理中的应用 T 3 ?a 3a?a F 1T , Y ? , VYY= , Y0 a aT vb , V Fha F Q26b Fh1“l aaa T , sh ) S H , F = 4 , i3MEh8B S b Song
5、 27 vF h8 SQ , ?CF- c 0?d 4 H ) (Fusarium) ) (Alternaria) 3b 3.3 脂肪氧合酶在工业中的应用 _V“ V 3 , 9 V ap a!4a oY9 4 , = 3 , = E b b 3T vF 3 , v# , #c J F%V aPa MbyN , 9|b Borrelli32V , LOX l . aYxY i , 74 F , Yl M T33,34b 6Bt , LOX LOX 8 Vrh Q , h . M , V7 % , h J n35b H LOX$ i01ZEB , 1 %F V4b LOX % T , 8 b ,
6、 LOX ?T 3“d , 71 xT31, LOX L V1y0 , ?b 3V1802 J _ 4 , T , s0 , s0B% , % ivv9F , 7 S 1 P , s ir , P y8 36b 6 , LOX b 31aVad= ?8 DNAaRNAa / 31b ?WQ , Z 3T37b 5 脂肪氧合酶的提取 4 | ZE1 4Ea E# E 34,5,27,38b 5.1 水浸提法 4E Y Ab|vx , B HW , | bA YAb 4E q Vr 85%, Ac bNZEVe , h TV , yNY A9 b328x5F , s , Y A9 6.71106U
7、/g , 1 8.61105U/mLY Ab 5.2 盐析法 E 1 ZE , “ - s , V HHP ) ?A LOX bN , Cs0 3/ m, Vs0 U LOX 2aF#% T , 7ZEeHoF “d , !9z e ZE , i P 6T , “ ? LCKv C LN11b | ZEb 2 jS , # , d , . 7 J J. J S , 2013, 34(3): 198201. Liu FG, Jin BQ, Niu LY, et al. Lipoxygenase activity of fresh corn J. Food Sci, 2013, 34(3): 19
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