1、TheilrStage1-One-clgFertilzedone-clstage(embryo),zonapeucapr(hownird).0-.9d(rnge0-2.5)ScalebrimicrosTheilrStage2-DivdingeDivdingestage,zonapelucidapresnt(hownr).Firclvorstbou24urs.1dpc(range1-2.5)2-4ls.ScalebrinmicronsTheilrStage3-MorulaFour-to16-celstagembryosfrmearlytofulycmpadru.Zonplucidpsn(hwni
2、red).Ebyithovidtwrutr-tbaljuntio2dpc(range1-3.5)4-16ls.Alscalebrsinmicrons.TheilrStage4-Blastocyt(ICMaprent)Embryoprgesfromrulatoheblastocytstilwithntzonapelcid(swnird).Therisearvdcfthbtelicvty3pc(range2-4)16-40ompctdels.Alscalebrsiniron.Intheblastocytsage(zona-itc)therisadiscinrelmdourlayoftroptd.U
3、ulytdintutrinelume.TheilrStage5-Blastocyt(zona-fre)Blastocyt(zona-fre),invariblyocatedwithnheurielum.4dpc(rang3-5.)SlebriicronsTheilrStage6-AtachmentofblastocytBlastocytsimplants,firtevidnceofembrnedoclsorigthltlicurffthilas.4.5dpc(range4-5.)WitshiSt8(ratCarnehumn)SclbrTheilrStage7-Implantioandforma
4、tinofEgCylindEctoplacentlcoeaprs.Rapidncreasinhumbrfincelmalledigthtiofthibtwihsbqenrowregcylir.Tpxialviscralls(opsitidfrtherophbticap)areubalns.Pimyendormlinthmrtopctdr.5dpc(range4.5-6)WitshiSt10(ratCrehumn)ScalTheilrStage8-DiferntiaofegcylinderImplantiosite2x3m.Theatrnltisueisivdebyrphblast(priy)g
5、icandthctlcntcoinvdbmtrlblo.Difreiftheclindeioeryadxtrabryoirgosathefmtinofthpomnitavity.Reicrsbre,wicis-celurndetdbythedistaldrfrtps.6pc(rnge5-6.)WitshiSta10(rat)CarehumnSclbrinicrosTheilrStage9-AdvancedEometrialRectionPS:Advancedg-cylinderstagewithefirsteiofamboicxi.Trclamorphlilistitntmbryoniandx
6、tebryncetdr.hecpletlcisfuivabyatlodahoriglofthutrinecrpsdaperd.ES:Ltinthistagestrulationbegis,producgefrmodcl.6.5(ran6.25-7.)DowsfolimbudThepostrineuropeformandthecndafthflibucosat8-12thsitpir.Aiscodesationfedlbbudpearsjuedoftstage.Thfbinveiclubiviinotelnchlcdphalcvel.Abst:ilimb,Rtkespuch.9.5dpc(ran
7、ge9-10.25)21-2somitpirsWithiSt17(atrCarnegaeghumn-stagetbl)Alsclbrsinicro.ikoaefalreversionTheilrStage16-Closurefposterineurope;hindlimbudntilbdTheindlimbudecomsviibleattlveofth23r-28thts.Thaibuapesarupndte3rd4tbncialcearisnctlyoncve.Rtksothapresrfm.Atheedftitagthpoteirurobicls.Absent:hiandltail.10d
8、pc(range9.5-10.7)3-34somitpirsWithiSt2-21(atrCaregaeg3ls),14(atls),15(tls)(un-tgetbAlcalebrimicros.Cikonagefalreversion.TheilrStage17-DeplnsidentaioThemostbviusditnguishngfaturarehepoftelspi,winowifitrore-lkpig,andhefistacefthpysiolgcalumbilalrni.T1tbrnirchsopuoldivetoxilrydmailaropnet.Thersncddevl
9、oftbitubeathetaigatsnd.Absent:lpit.10.5dpc(range10-.25)3-9somitpirsWithiS24-(rat)Carnegtage13ls,14(atls),15(tls)(un-tgetblAlcbriicro.Cikonamgefalrvrsion.TheilrStage18-ClosureflnsveiclTheprimayextrnalyrecognisablefatureistogsivclosufthlvsicl.Thsitinthrilrirongrvbleadeapdgwtftebraitrikg.Tslitsarm.Asnt
10、:uitoryhock,ntiorftple.1dpc(range10.5-.2)40-somitpirsWithiS2-6(rat)Carnegtage13ls,14(atls),15(tls)(un-stgetbl)Aclbriicron.ClikonamfalrversionTheilrStage19-LensvicleompletlysepartdfromsufcThelnsveiclbecomscopletlyclosedandtacdfrothtder.Thriphrlmrgi ywldfn.Tfoelbsrentobivitowregions,thprxialprcstngfhe
11、fuli-gdmaderpriralprtwcforsirulropl-sadhanplte(antftl).Themilnltrmrgioheicircoigtoethreducithtrtoanwsladatoryilcksbmvisble.Absent:rtinalpigentaio,ignsofigers.1.5dpc(range1-2.5)4-7somitpirsWithiS26-7(rat)Carnegtage1l,(human-stgetabl)Alsclbrsinicrons.ikonamgefalrgevrsion.TheilrStage20-Earliestsignsoff
12、ingsPreviousStageStageIndxStageCriteaAntomyNextSageTheandplte(antrioftplae)isnolgercirulrbvlpsglswhicrpdtoftigt.Thetroftlaisalsdinushablfromleroftheeg.Itpoietstpigntipimelyrfrenathroutransrtcna.Thtodbraiveiclalrlvisbl.Absnt:5rowsfhiker,indethandplte.12dpc(range1.5-3)48-5somitpirsWithiS28(at)Carnegta
13、ge17ls,(human-stgetbl)Alscalersinmicrons.CikonagefalrgevrsionTheEdinburghMouseAtlas:ilrStae21-nrioftplaeindtePviousStageStageIndxSgCriaAntomyNextSageThedistalbordesoftheantrioandposterifotpl nwidtheigwdhsancticabiscr.Tlbanwrisareoidetfale.pinrpilydevlopnfrmrtrightalestoheh.Firwsvibsvsibwlasarmiathif
14、olcerteyndtroveter.Tniclhalotislumen.ThpysiolgialubilripritAbent:hirflices,ditalyseartfingers.13dpc(range12.5-4)52-somitpairsWitshiSt9-30(t)CarnegiSage18(al),1l,(human-tgetblAlsclrsinicrons.Clickonaimfoalgevrion.TheEdinburghMouseAtlas:ilrStae2-FingersseprteistlyPreviousStageStageIndxStageCriteaAntom
15、yNextSageIndivualfingersavisbleinthanteriofotpldthrdpdsbewhowicotysprtd.Thlngbesftelimbsarenatrairflinhpctl,lvicukeo.Thpiaiurndforwdsthumbilclersiu.Absent:airfoliinthecpalicregion.14dpc(range13.5-)56-0somitpairsWitshiSt(t)CarnegiSage2(al),21la),2(tls),23(tl),un-stgetblAlscbrsiicro.Clickonaimgefoalvr
16、ion.TheEdinburghMouseAtlas:TheilerStage23-ToesseparatePreviousStageStageIndxStageCriteaAntomyNextSageThetosepartndareclarlydivergnt,otbcmingleutil.Hfolicsareprthcprgionbuttheihyofvibrsa.Thepavrmortanltextnluditrytsndteylidrlon.Absent:ailprimoia,figers2-5parle.15dpc60somiteairsWitchiSg32(t)Huanftlpri
17、od.Alsclebrsiicrns.Clickonaimgfoalgevrion.TheEdinburghMouseAtlas:TheilrStag24-RepitnfmbiclherniaPrvouseeIndxSageCreiaAntomyNxtSgeFingers2-5arenrlyparle.Nailprimodiaaviblothos.Thyldshvefusimtcsbedofttgnthpnaltcmpltlcverxraluditoryeau.Thubilalhisisingdterisorspndgncreathsizeoftpeitlac.Abst:fingerandts
18、jindtogethr.16dpc0SomiteairsWitschig3(t)Alalebrsinicron.Clickonifoalgevrsion.TheEdinburghMouseAtlas:ilrStae25-SkinwrikledPreviousStageStageIndxStageCriteaAntomyNextSageTheskinhastickendaformedwrinklesandtubcousvislsvb.Thfigrdthbcparltmilalerniadiaed.eylidshvfus.Wskrjustvib.Absent:arextndigoveraditor
19、ymeatus,lowhisk.17dpcWitschiStage34(rat)Allebrsinmions.Clickonaimfolrgevrion.TheEdinburghMouseAtlas:ilrStae26-LngwhiskersPreviousStageStageIndxStageCriteaAntomyNextSageThewiskervisbleatsge25aredfintelylongradthnhickn.Thpaisauctirulyoftlumoftheitorymeasvibe.Tyesrbrelyvsiblthrogthclsdli18dpcWitschitag
20、e35(rat)Allebrsinmions.Clickonaimfolrgevrion.TheEdinburghMouseAtlas:ilrStae27-NewbornmusePreviousStageStageIndxStageCriteaAntomyNextSageNewborn19-20dpcAlscalebrsinmirons.Clickonthigfoalgevrion.Mousembryoscanbestagdcordingtoavrietyofcriteia,themostgenralofwhicarethdciedThilrin“TheHuseMus:AlasMusDvlp“
21、(Spiger-Vlg,NewYork,1 9 8 9 ).eilscitriatobrdtoitngihanyoftheiortanhasofarlydvelpmntadmuhefresuplemdbyters,rxample,clumber,somitnubrohsecartiscdbyDownsaDvi(1 9 9 3 )Devlont,1 1 8 ,1 2 5 5 .Wehavtefbindheiferntcrieithebl1 lowhicdfiesawsetofstgbsdontheumrTilsi,buiintrmediativsioniictdbyn-iertagbrs.Eby
22、osftheamgestaiolgyiferitheirsageofdvlopmn.Wehvtefrincludintblnidicationthxpcdngestailags(daysofgstaio,p)overwhiceahevlpmealsgemaybefoud.Difrntouetrinevltdifrntasnd,isocshowdifrncsitheelaivsfdvlopmtofieog.Stricly,thetagrecgnisebyDowandisplytoutbreichPOstrain.ThedainrmindofthalelrfetoembrfcrstwenF1 yb
23、id(C5 7 BLXCBA)ice.EachTeilrstageislnked(clikontheumber)toiscrespondigiarm,withoredtailsoftdfinfturfrtasg.Aifxitialiextoediagsiloprvie.TheilrStagdpc(range)2 2 2 2 SomiteN.3 3 3 3 Clnuber(C5 7 BLxA)F15 7 15 7 15 7 1 mice4 4 4 4 POmice5 5 5 51 0 -0 .9(0 2 .5 ) 1 One-clg2 1 (1 -2 .5 ) 2 -4 Divdinge3 2
24、(1 -3 .5 ) 4 -1 6 Morula4 3 1 6 -4 0 Blstcyt,Inerclmasparent(2 -4 )5 4 (3 -5 .5 ) Blastocyt(zona-fre)6 4 .5(4 -5 .5 ) Atchmetfblastocyt,primayendorcovrslelisufceoficela7 5 (4 .5 -6 ) ImplantiondformatinofegcyliderEcplacetlceaprs,nlagibls,riayndorlinemurltophectdm8 6 (5 -6 .5 ) Difeniaofegcylinder.Ip
25、ltiosite2 x3 .Etoplatlconerginivadbymaernlbld,Rihtsmernedpoitccaviyfor9 a6 .5(6 .2 5 -7 2 5 ) Pre-stak(PS),advncendometrialcion,ectoplcetloivabybld,xrambrynicectodr,emryonicisvile, PS9 b Ealsteak(ES),gastrultionstar,firstevidncofsdemES1 0 a7(6 .5 -7 7 5 ) Mistreak(MS),aniotcfldstartoform MS1 0 b Lat
26、esak,nobud(LSOB),exoclmL1 0 c rerly(E),alnticbudfirstaps,nod,amnioclsig1 1 a7 .5(7 .2 5 -8 ) Neullate(NP),headprocesdvloping,ioncplt OB1 1 b Lateuralte(LP),elongaedlantoicbd EB/L1 1 c Earlyheafold(EHF) HF1 1 d Ltfl(L),foregutinvagitionL1 2 a8 (7 .5 -8 7 5 )1 -4 1 -41 41 41 4 somites,alntisxeds,1bran
27、chilarch,rrtofrm,foregutpoketvisble,poticsul(a2 -3 sistag)1 2 b 5 -7 5 -75 75 75 7 omites,alntoiscntaschorinatthendfTS1 2Abst2 arch,7 somites1 3 8 .5(8 -9 .2 5 ) 8 -1 2 Turnigoftebry,1 branchilarchhasmxilaryndaiulopents,2 ndarchpresntAbset3 dbachilarch1 4 9 (8 .5 -9 7 5 )1 3 -2 0 Formaintheinteaslro
28、cstartfrmAbsenthidiltgifThilrsag9 -1 2cutesyofK.Lwsopersolcuication.5 .FrmDn,M.andDvis,T.(1 9 9 3 )Sgiofgastrultingmousebryosophlgicalmrksithedictingmicrspe.Dvlpe,1 1 8 1 2 5 5 -1 2 6 6 .CoentsGralcomentoting(dpc):InjudgithelowranduperangesofdpcequivalentoaptiulThilrsae,whaveralyfldTilesbokd,imtas,h
29、givawiderangetnTilr,bcusthenumbersofryhcitesarsl.Wevienalrghmaxitani cause,ingrl,byoarorliklytobetardebyteirnviroeorgenticontiiothamdetpcedmequiclthrougvlopt.Istcas,hwvr,hersulingpcrangisanstiathiscnsitewithersulofTheilr,butnobasdonaditolevide.Comonsmitnbrs:agefmiteubrsfachstgisivenolyasguidethatighexpctedoftypiclbryos.AcanenfromTeilr(1 9 8 9 )4 therurangecabemuchwider.Threfo,fralstgeaftrTS1 2 ,thesomitenubershouldntbetksrlilglobalinicattheovlmbyosag.RichardBlock,JthBrd,DuncaidsnadKirtieLwson,7 thMay1 9 9 8PlesmilensoPf.JothBr
