蛋白质浓缩过滤器-.doc

1、Amicon Ultra蛋白質濃縮過濾器- 流速流速與 回收率回收率, 兩者兼具最新一代的蛋白質濃縮過濾離心管 Amicon Ultra, 同時結合新穎的超低蛋白吸附 Ultracel超濾膜及創新的掃流式設計,可兼具高流速及高樣品回收率。 應用範圍1. Cell culture broths, Cell lysates, Columneluents 的樣品濃縮 (減少樣品體積)。2. 以 MS 分析 Serum peptide (ex. disease marker) 之前除去干擾的蛋白質。3. 除鹽、換 buffers。4. 除去反應中多餘的 ligands/labels/reactants

2、。 超快速的樣品處理 超高的濃縮倍數 只需 15 分鐘即可處理 4-15 mL 的樣品。 一般可達到 80-100 倍的濃縮倍數。Amicon Ultra 4產品規格：Typical Protein Recovery 回收率：MembraneNMWL, Da Protein SoluteProtein RetentateVolume, mLProtein RetentateRecovery, %5,000 Cytochrome c, 12,400 Da, (0.25 mg/mL) 0.09 9410,000 Cytochrome c, 12,400 Da, (0.25 mg/mL) 0.20

3、9530,000 BSA, 67,000 Da, (1 mg/mL) 0.20 9550,000 BSA, 67,000 Da, (1 mg/mL) 0.08 94100,000 IgG, 156,000 D, (1 mg/mL) 0.34 914 mL starting volume in a fixed angle rotor at 7,500 x g at 25C. Spin times: 5K (20 min); 10 and 30K (10 min); 50K (5 min); 100K (15 min).其他規格：Maximum Initial Sample Vomume 4mLT

4、ypical final concentrate volume 50uLCentrifugal Force - YM-100 Swinging bucket rotor: 4,000 x g for dilute samples; 3,500 x g for concentrated or viscous solutions. Fixed angle rotor: 7,500 x g Membrane Regenerated CelluloseDevice Housing: Styrene/butadiene; Tube: Polypropylene; Cap and Liner: Polye

5、thylene訂購資訊：Product Name NMWL, kDa Qty/Pk Catalogue No.Amicon Ultra-4 PLCC Centrifugal Filter Unit 5 8 UFC8 005 08Amicon Ultra-4 PLCC Centrifugal Filter Unit 5 24 UFC8 005 24Amicon Ultra-4 PLCC Centrifugal Filter Unit 5 96 UFC8 005 96Amicon Ultra-4 PLGC Centrifugal Filter Unit 10 8 UFC8 010 08Amicon

6、 Ultra-4 PLGC Centrifugal Filter Unit 10 24 UFC8 010 24Amicon Ultra-4 PLGC Centrifugal Filter Unit 10 96 UFC8 010 96Amicon Ultra-4 PLTK Centrifugal Filter Unit 30 8 UFC8 030 08Amicon Ultra-4 PLTK Centrifugal Filter Unit 30 24 UFC8 030 24Amicon Ultra-4 PLTK Centrifugal Filter Unit 30 96 UFC8 030 96Am

7、icon Ultra-4 PLQK Centrifugal Filter Unit 50 8 UFC8 050 08Amicon Ultra-4 PLQK Centrifugal Filter Unit 50 24 UFC8 050 24Amicon Ultra-4 PLHK Centrifugal Filter Unit 50 96 UFC8 050 96Amicon Ultra-4 PLHK Centrifugal Filter Unit 100 8 UFC8 100 08Amicon Ultra-4 PLHK Centrifugal Filter Unit 100 24 UFC8 100

8、 24Amicon Ultra-4 PLHK Centrifugal Filter Unit 100 96 UFC8 100 96Amicon Ultra 15產品規格：Typical Protein Recovery 回收率：MembraneNMWL, Da Protein SoluteProtein RetentateVolume, mLProtein RetentateRecovery, %5,000 Cytochrome c, 12,400 Da, (0.25 mg/mL) 0.14 91.0810,000 Cytochrome c, 12,400 Da, (0.25 mg/mL) 0

9、.24 93.0330,000 BSA, 67,000 Da, (1 mg/mL) 0.30 97.9450,000 BSA, 67,000 Da, (1 mg/mL) 0.17 93.32100,000 IgG, 156,000 D, (1 mg/mL) 0.28 89.0315 mL starting volume in swinging bucket rotor at 4000 x g at 25 C. Spin times: 5K (45 min); 10K and 100K (20 min); 30K (10 min); 50K (15 min).其他規格：Maximum Initi

10、al Sample Vomume 15mLTypical final concentrate volume 200uLCentrifugal Force - YM-100 Swinging bucket rotor: 4,000 x g for dilute samples; 3,500 x g for concentrated or viscous solutions. Fixed angle rotor (35): 7,500 x g Membrane Regenerated CelluloseDevice Housing: Styrene/butadiene; Tube: Polypro

11、pylene; Cap and Liner: Polyethylene訂購資訊：Product NMWL, kDa Qty/Pk Catalogue No.Amicon Ultra-15 PLCC Centrifugal Filter Unit 5 8 UFC9 005 08Amicon Ultra-15 PLCC Centrifugal Filter Unit 5 24 UFC9 005 24Amicon Ultra-15 PLCC Centrifugal Filter Unit 5 96 UFC9 005 96Amicon Ultra-15 PLGC Centrifugal Filter

12、Unit 10 8 UFC9 010 08Amicon Ultra-15 PLGC Centrifugal Filter Unit 10 24 UFC9 010 24Amicon Ultra-15 PLGC Centrifugal Filter Unit 10 96 UFC9 010 96Amicon Ultra-15 PLTK Centrifugal Filter Unit 30 8 UFC9 030 08Amicon Ultra-15 PLTK Centrifugal Filter Unit 30 24 UFC9 030 24Amicon Ultra-15 PLTK Centrifugal

13、 Filter Unit 30 96 UFC9 030 96Amicon Ultra-15 PLQK Centrifugal Filter Unit 50 8 UFC9 050 08Amicon Ultra-15 PLQK Centrifugal Filter Unit 50 24 UFC9 050 24Amicon Ultra-15 PLQK Centrifugal Filter Unit 50 96 UFC9 050 96Amicon Ultra-15 PLHK Centrifugal Filter Unit 100 8 UFC9 100 08Amicon Ultra-15 PLHK Ce

14、ntrifugal Filter Unit 100 24 UFC9 100 24Amicon Ultra-15 PLHK Centrifugal Filter Unit 100 96 UFC9 100 96其他產品相關資訊：User Guide of Amicon Ultra-4User Guide of Amicon Ultra-15Urine Concentration Prior to Agarose Gel Electrophoresis and Immunofixation ElectrophoresisClinical Chemistry Product Selection Guide