1、各种激发光和吸收光波长现在发现一个更全的有关激发光和发射光的波长图谱。这对 CONFOCAL 去做多重染色时是必不可少的参考资料。全部文件请到这里下载！Excitation and Emission Wavelengths of FluorophoresTavis “FluoroTable“: Common fluorophores (from Zeiss Corporation website)Fluorophore Absorption Emission Other info1,5 IAEDANS 336 4901,8-ANS 372 4804-Methylumbelliferone 38
2、5 5025-carboxy-2,7-dichlorofluorescein 504 5295-Carboxyfluorescein (5-FAM) 492 5185-Carboxynapthofluorescein (pH 10) 512/598 563/668 Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)542 5685-FAM (5-Carboxyfluorescein) 492 5185-HAT (Hydroxy Tryptamine) 370-415 520-5405-Hydroxy Tryptamine (HAT) 370
3、-415 520-5405-ROX (carboxy-X-rhodamine) 578567604591 5-TAMRA (5-Carboxytetramethylrhodamine)548542552568 6-Carboxyrhodamine 6G 518 5436-CR 6G 518 5436-JOE 520 5487-Amino-4-methylcoumarin 351 4307-Aminoactinomycin D (7-AAD) 546 6477-Hydroxy-4-methylcoumarin 360 449, 4559-Amino-6-chloro-2-methoxyacrid
4、ine 412, 430 471, 474ABQ 344 445Acid Fuchsin 540 630ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430 471, 474Acridine Orange + DNA 502 526Acridine Orange + RNA 460 650Acridine Orange, both DNA & RNA 440-480 520-650Acridine Red 455-600 560-680Acridine Yellow 470 550Acriflavin 436 520Acriflavin Feulg
5、en SITSA 355-425 460Aequorin (Photoprotein) 466 PhotoproteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFPAlexa Fluor 350 346342442441 Alexa Fluor 430 431 540Alexa Fluor 488 495, 492 519, 520Alexa Fluor 532 531, 532 553, 554Alexa Fluor 546 556, 557 572, 573Alexa Fluor 56
6、8 577, 578 603Alexa Fluor 594 590, 594 617, 618Alexa Fluor 633 632 650Alexa Fluor 647 647 666Alexa Fluor 660 668 698Alexa Fluor 680 679 702Alizarin Complexon 530-560, 580580, 624-645Alizarin Red 530-560 580Allophycocyanin (APC) 630, 645 655, 660AMC, AMCA-S 345 445AMCA (Aminomethylcoumarin) 345347425
7、444 AMCA-X 353 442Aminoactinomycin D 555 655Aminocoumarin 346350442445 Aminomethylcoumarin (AMCA) 345347425444 Anilin Blue 600Anthrocyl stearate 360-381 446APC (Allophycocyanin) 630, 645 655, 660APC-Cy7 625-650 755APTRA-BTC = Ratio Dye, Zn2+ 466/380 520/530 Ratio Dye, Zn2+APTS 424 505Astrazon Brilli
8、ant Red 4G 500 585Astrazon Orange R 470 540Astrazon Red 6B 520 595Astrazon Yellow 7 GLL 450 480Atabrine 436 490ATTO-TAG? CBQCA 465 560ATTO-TAG? FQ 486 591Auramine 460 550Aurophosphine G 450 580Aurophosphine 450-490 515BAO 9(Bisaminophenyloxadiazole) 365 395BCECF (high pH) 492, 503 520,528BCECF (low
9、pH) 482 520Berberine Sulphate 430 550Beta Lactamase 409 447, 520BFP blue shifted GFP (Y66H)Blue Fluorescent Protein381, 382, 383445, 447, 448blue shifted GFP (Y66H)Blue Fluorescent ProteinBFP/GFP FRETBimane 398 490Bisbenzamide 360 461Bisbenzimide (Hoechst) 360 461bis-BTC = Ratio Dye, Zn2+ 455/405 52
10、9/505 Ratio Dye, Zn2+Blancophor FFG 390 470Blancophor SV 370 435BOBO?-1 462 481BOBO?-3 570 602Bodipy 492/515 490 515Bodipy 493/503 533 549Bodipy 500/510 509 515Bodipy 505/515 502 510Bodipy 530/550 528 547Bodipy 542/563 543 563Bodipy 558/568 558 569Bodipy 564/570 564 570Bodipy 576/589 579 590Bodipy 5
11、81/591 584 592Bodipy 630/650-X 625 642Bodipy 650/665-X 647 665Bodipy 665/676 605 676Bodipy Fl 504, 505 511, 513Bodipy FL ATP 505 514Bodipy Fl-Ceramide 505 511Bodipy R6G SE 528 547Bodipy TMR 542 574Bodipy TMR-X conjugate 544 573Bodipy TMR-X, SE 544 570Bodipy TR 589 617Bodipy TR ATP 591 620Bodipy TR-X
12、 SE 588 616BO-PRO?-1 462 481BO-PRO?-3 544 570Brilliant Sulphoflavin FF 430 520BTC - Ratio Dye Ca2+ 464/401 533/529 Ratio Dye Ca2+BTC-5N - atio Dye, Zn2+ 459/417 517/532 Ratio Dye, Zn2+Calcein 494 517Calcein Blue 373 440Calcium Crimson 588, 589 611, 615Calcium Green 501, 506 531Calcium Green-1 Ca2+ D
13、ye 506 531 Ca2+ DyeCalcium Green-2 Ca2+ 506/503 536 Ca2+Calcium Green-5N Ca2+ 506 532 Ca2+Calcium Green-C18 Ca2+ 509 530 Ca2+Calcium Orange 549 575576 Calcofluor White 385, 395, 405437, 440, 445Carboxy-X-rhodamine (5-ROX) 576 601Cascade Blue 377398399420423 Cascade Yellow 399400550552 Catecholamine
14、410 470CCF2 (GeneBlazer)CFDA 494 520CFP - Cyan Fluorescent Protein 430, 433, 436, (453)474, 475, 476, (501)Cyan Fluorescent ProteinCFP/YFP FRETChlorophyll 480 650Chromomycin A 436-460 470Chromomycin A 445 575CL-NERF (Ratio Dye, pH) 504/514 540 Ratio Dye, pHCMFDA 494 520Coelenterazine Ca2+ Dye, biolu
15、minescence (429) 465 Ca2+ Dye, bioluminescence, native moleculeCoelenterazine cp (Ca2+ Dye,) (430) 442 Ca2+ Dye, bioluminescenceCoelenterazine f (437) 473 Ca2+ Dye, bioluminescenceCoelenterazine fcp 452 Ca2+ Dye, bioluminescenceCoelenterazine h (437) 464 Ca2+ Dye, bioluminescenceCoelenterazine hcp (
16、433) 444 Ca2+ Dye, bioluminescenceCoelenterazine ip 441 Ca2+ Dye, bioluminescenceCoelenterazine n (431) 467 Ca2+ Dye, bioluminescenceCoelenterazine O 460 575Coumarin Phalloidin 387 470C-phycocyanineCPM Methylcoumarin 384 469 MethylcoumarinCTC 400-450 602CTC FormazanCy2 489 506Cy3.1 8 554 568Cy3.5 58
17、1 598Cy3 514552554566570 Cy5.1 8 649 666Cy5.5 675 695Cy5 649 666670 Cy7 710, 743 767, 805Cyan GFP 433 (453) 475 (501)cyclic AMP Fluorosensor (FiCRhR) 500 517CyQuant Cell Proliferation Assay 480 520 Cell Proliferation AssayDabcyl 453Dansyl 340 578Dansyl Amine 337 517Dansyl Cadaverine 335 518Dansyl Ch
18、loride 372 518Dansyl DHPE 336 517Dansyl fluoride 356 noneDAPI 359 461Dapoxyl 403 580Dapoxyl 2 374 574Dapoxyl 3 373 574DCFDA 504 529DCFH (Dichlorodihydrofluorescein Diacetate)505 535DDAO 463 607DHR (Dihydorhodamine 123) 505 534Di-4-ANEPPS 496 705Di-8-ANEPPS (non-ratio) 488498605713 DiA (4-Di-16-ASP)
19、456 591Dichlorodihydrofluorescein Diacetate (DCFH)505 535DiD - Lipophilic Tracer 644 665 Lipophilic TracerDiD (DiIC18(5) 644 665DIDS 341 415Dihydorhodamine 123 (DHR) 505 535DiI (DiIC18(3) 549, 551 565Dinitrophenol 349DiO (DiOC18(3) 484, 487 501, 502DiR 748 780 Lipophilic TracerDiR (DiIC18(7) 750 779
20、DM-NERF (high pH) 497/510 540 Ratio Dye, pHDNP 349Dopamine 340 490-520DsRed 558 583 Red fluorescent proteinDTAF 494 520DY-630-NHS 621 660 Hemicyanelabel for proteins and DNADY-635-NHS 634 664 Hemicyanelabel for proteins and DNAEBFP 383 447 Enhanced Blue Fluorescent ProteinECFP 436 474 Enhanced Cyan
21、Fluorescent ProteinEGFP 488, 498 507, 516 Enhanced Green Fluorescent ProteinELF 97 345 530Eosin 524 545Erythrosin 529, 532 554, 555Erythrosin ITC 529 555Ethidium Bromide 510, 523 595, 605Ethidium homodimer -1 (EthD-1) 528 617Euchrysin 430 540EukoLightEuropium (III) chlorideEYFP 513, 520 527, 532 Enh
22、anced Yellow Fluorescent ProteinFast Blue 360 440FDA 494 520Feulgen (Pararosaniline) 570 625FIF (Formaldehyd Induced Fluorescence) 405 433FITC 490, 494 520, 525FITC Antibody 493 517Flazo Orange 375-530 612Fluo-3 480 ?506, 506520, 527Fluo-4 494 516Fluorescein (FITC) 490, 494 520, 525Fluorescein Diace
23、tate 494 520Fluoro-Emerald 495 524Fluoro-Gold (Hydroxystilbamidine) 361 536Fluor-Ruby 555 582FluorX 494 520FM 1-43 479 598FM 4-46 515 640Fura Red?(high pH) 572 657Fura Red?Fluo-3Fura-2, high calcium 335 505 Excitation ratio dyeFura-2, low calcium 363 512 Excitation ratio dyeFura-2/BCECFGenacryl Bril
24、liant Red B 520 590Genacryl Brilliant Yellow 10GF 430 485Genacryl Pink 3G 470 583Genacryl Yellow 5GF 430 475GeneBlazer (CCF2)GFP (S65T) 498 516GFP red shifted (rsGFP) 498 516GFP wild type, non-UV excitation (wtGFP) 475 509GFP wild type, UV excitation (wtGFP) 395 509GFPuv 385 508Gloxalic Acid 405 460
25、Granular Blue 355 425Haematoporphyrin 530-560 580Hoechst 33258 345 487Hoechst 33342 347Filter sets installed on the Axiovert 200M:1) Brightfield DIC:filter cube contains DIC analyzer2) DAPI / BFP: Chroma set 31041, Ex at 380 nm (bandwidth 30 nm), Em at 460 nm (bandwidth 50 nm).3) FITC / GFP: Chroma
26、set 41001, Ex at 480 nm (bandwidth 40 nm), Em at 535 nm (band width 50 nm).4) RHOD: Chroma set 41002c, Ex at 545 nm (bandwidth 30 nm), Em at 620 nm (bandwidth 60 nm). 5) Cy5: Chroma set 41024, Ex at 620 nm (bandwidth 60 nm), Em 665 nm and above (long pass filter). Also available upon request:4) GFP:
27、 Chroma set 41017, Ex at 470 nm (bandwidth 40 nm), Em 525 nm (band width 50 nm).5) CFP: Chroma set 31044v2, Ex at 436 nm (bandwidth 20 nm), Em 480 nm (bandwidth 40 nm).6) YFP: Chroma set 41028, Ex at 500 (bandwidth 20 nm), Em 535 (bandwidth 30 nm).7) CFP/YFP Chroma set 31052, Ex 436 (bandwidth 20 nm), Em 535 (bandwidth 30 FRET: nm).
