1、This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller
2、and DS18B20 temperature sensor.血流感染实验室诊断新进展北京协和医院检验科王瑶2013年 4月 13日This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomput
3、er. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.内容 什么是血流感染？ 血流感染流行病学特点 血培养流程优化 提高阳性率 分析培养结果 减少污染 分子生物学方法检测血流感染 基于 PCR的技术 MALDI-TOF MS PNA-FISHThis paper mainly introduces the design of an intelligent temperature control system which realizes the funct
4、ion of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.概念 感染炎症 +病原体 全身炎症反应综合征（ SIRS） 体温 38C或 90次 /分 呼吸频率 20次 /分，或 PaCO212109/L或 10% 脓
5、毒血症（ sepsis）感染 +SIRS，血培养可以阳性或阴性 重度脓毒血症（ severe sepsis） sepsis+脏器功能障碍、组织灌注不良和低血压 感染性休克（ septic shock）This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor D
6、S18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.概念 败血症（ septicemia）：病原菌及其毒素侵入血流所引起的临床综合征，是一种严重的全身感染。病原菌主要是细菌，也可为真菌、分枝杆菌等。 菌血症（ bacteremia）：细菌在血流中短暂出现的现象，一般无明显毒血症状，在国外文献中常与败血症通用。 血流感染（ bloodstream infection, BSI）：败血症和菌
7、血症目前统称为血流感染。This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 micr
8、ocontroller and DS18B20 temperature sensor.血流感染 医院获得性血流感染 原发血流感染 实验室证实血流感染（ Laboratory-confirmed Bloodstream Infection, LCBI） 临床血流感染（ Clinical Sepsis） 继发血流感染：血培养分离出有意义微生物，而且此微生物与另一部位之院内感染有关。唯不包括血管或血管内导管装置所引起之血流感染。 社区获得性血流感染This paper mainly introduces the design of an intelligent temperature control
9、 system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.实验室证实血流感染（ LCBI） 标准一：从一次或多次血标本中培养出一种一致的 致病菌 ，而
10、且培养出的病原体与其它部位的感染无关。 标准二：病人至少具有下列症状和体征之一：发热（ 38 C ），寒颤或低血压，并致病符合下列之一： 1.从 两次或两次以上不同部位 抽血的标本中培养出 皮肤寄生菌 （如类白喉杆菌、芽孢杆菌、丙酸杆菌、凝固酶阴性葡萄球菌或微球菌）。 2.至少一次从带血管内导管的病人血标本 中培养出 皮肤寄生菌群 （如类白喉杆菌、芽孢杆菌、丙酸杆菌、凝固酶阴性葡萄球菌或微球菌），并且主管医生开始了适当的抗菌药物治疗。 3.血中抗原检测阳性 （如流感嗜血杆菌、肺炎链球菌、脑膜炎奈瑟菌或 B群链球菌）。与实验室阳性结果一致的症状和体征与其它部位的感染无关。 标准三：年龄 38 C
11、 ），低温（ 37 C ），呼吸暂停、脉搏徐缓，并具有下列之一（同上 3点略）This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of
12、the system are AT89C51 microcontroller and DS18B20 temperature sensor.临床血流感染（ Clinical Sepsis）具有下列二条件之一者：具有非其它已知原因所引起的发烧、血压过低（收缩压 90mmHg或收缩压低于平常超过 40mmHg），少尿（每小时尿量低于 30ml）等临床症状任何一项，且符合下列所有条件者： 未做血培养，或血培养阴性或血液抗原反应呈阴性者 其它部位无明显感染者 医生针对此感染中毒症状给予适当抗菌药物治疗1岁以下婴儿 ，具有非其它书籍原因引起的发烧、体温过低、呼吸中止或心跳过缓等临床症状任何一项，且符合下
13、列所有条件者： 未做血培养，或血培养阴性或血液抗原反应呈阴性者 其它部位无明显感染者 医生针对此感染中毒症状给予适当抗菌药物治疗This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer
14、. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.BSI流行病学特点 BSI发病率逐年增加 凝固酶阴性葡萄球菌、肠球菌、真菌血流感染发病率逐年上升，革兰阴性菌下降 耐药菌比例逐年增加 社区获得与医院获得血流感染病原谱存在差异 院内 BSI患者病死率高This paper mainly introduces the design of an intelligent temperature control system which realizes the f
15、unction of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.美国脓毒血症流行病学+600%+300%+300%Martin et al, NEJM 2003;348:1546This paper mainly
16、 introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 tempe
17、rature sensor.加拿大 CANWORD监测 2002-2009Diag Microbiolo Infect Dis. 2011; 69: 307This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip
18、 microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.加拿大 CANWORD监测 2002-2009Diag Microbiolo Infect Dis. 2011; 69: 307This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature
19、 measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.美国 49医院 BSI病原谱CID. 2004, 39: 309-317This paper mainly introduces the design of an intelligent te
20、mperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.PUMCH2012年 834血培养分离株This pape
21、r mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B
22、20 temperature sensor.PUMCH2012年 144株 BSI大肠埃希菌药敏结果ESBL 60.2%This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. Th
23、e core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.PUMCH2012年 67株 BSI肺炎克雷伯菌药敏结果ESBL 34.4%This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus
24、 digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.PUMCH2012年 61株 BSI鲍曼不动杆菌药敏结果This paper mainly introduces the design of an intelligent temperature control system which realizes the function
25、 of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.PUMCH2012年 76株 BSI金黄色葡萄球菌药敏结果This paper mainly introduces the design of an intell
26、igent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.PUMCH2012年 46株 BSI草绿色
27、溶血链球菌药敏结果This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microco
28、ntroller and DS18B20 temperature sensor.2011CHIF-NET489株 BSI酵母菌This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer.
29、 The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.2011CHIF-NET念珠菌对氟康唑的敏感性This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital tem
30、perature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.2011CHIF-NET念珠菌对伏立康唑的敏感性This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature
31、measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.2011CHIF-NET其他酵母菌对氟康唑的敏感性This paper mainly introduces the design of an intelligent temperature co
32、ntrol system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.2011CHIF-NET其他酵母菌对伏立康唑的敏感性This paper main
33、ly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 tem
34、perature sensor.采血量是影响血培养阳性率的独立相关因素 CLSI M47-A：每位患者采集 23套血培养（4060ml） Surviving Sepsis Campaign Guidelines (Worldwide)：抗菌药物治疗前至少采集 2套血培养 英国 NHS血培养标准：采集 2套血培养This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and
35、control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.采血量对血培养阳性率的影响JCM 2007This paper mainly introduces the design of an intelligent temperature control system which r
36、ealizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.采血量对血培养阳性率的影响J. Clin. Microbiol. 2011, 49(12):4047This paper
37、mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20
38、 temperature sensor.J. Clin. Microbiol. 2011, 49(12):4047 Routine Set Mayo Clinic:2 BACTEC Aerobic Plus Bottles + 1 BACTEC Anaerobic Lytic Bottle采血量和阳性率非条件致病菌27This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement
39、and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor. 在 多 次 血培养中 单次 培养下列 细菌 阳性 凝固酶阴性葡萄球菌 棒状杆菌 微球菌 丙酸杆菌 芽孢 杆菌 如多次培养阳性，可能为条件致病菌，需结合临床分析血培养污染菌28This paper mainly int
40、roduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperatu
41、re sensor.非条件致病菌Mayo Clinic(p0.001)为什么需氧 +厌氧？Pathogen No.两种组合均能检测不同的病原菌J. Clin. Microbiol. 2011, 49(12):4047 29This paper mainly introduces the design of an intelligent temperature control system which realizes the function of temperature measurement and control by using single bus digital temperature sensor DS18B20 and single chip microcomputer. The core components of the system are AT89C51 microcontroller and DS18B20 temperature sensor.表皮葡萄球菌血培养阳性意义CID 1997
