1、 l :2005-12-21Te:(1976-), 3, i ,Z 2v 3 S j q,p V.24 2 Z 2v1 SVo.l 24No. 22006 M6NATURAL SCIENCE JOURNALOF HAINAN UNIVERSITY Jun. 2006cI|:1004-1729(2006)02-0194-07ACC yZ陈银华1, 2,黄 伟3,王 海1(1.Z 2v 3 S j,Z 2Z g570228;2. j #sE8y 0yI NVr,?C3 5- u13- uT L VE8yVr1, YQ1 u-2181 -1088 u, s?CQ uE4y 0 YQ u(-193 -
2、86)i 11.VhM1yPRB-16 0 ukTAAGAGCCGCC + ysYqs12.PT LACSy 0 uI NPGy、E8y、T L+syM3763, TT LACOy、PGy# %PGy 0 M1 , M 21. 9%25. 0%. 0 ?CI NE4、E8#YsqMB , ?VrV ,y5 0 ?,yPT L+s c.196Z 2v1 S 2006 M 3 ACOyVrZ3. 1 ACOyVry0 ACOyVr s 、 、 3V. ,ACOy Y、ABAIAA. 41ACC、 =、Cu2 +、LiCl、CHI、CHX、NaCl.Y) E 2、 、C(、5 N、 ACOyVr;A
3、BA5 ACCOX1ACCOX2Vr13 ;NaCl) ? ACO1Vr;7IAA V(ACOyVr.KV , IAA ?Y9F,1 YVY1 HACOy 、2 、h8 S 、;、l s. O3ACCOX1ACCOX2、I NACO1、C(ACO1、%ACOyVr 15 ;) I N= V y ACOyVr;h8 S VC(CmACO1CmACO3yVr16. 6,1 Hq/ 7、.x、T L 、 V ( VACOyVr, 、P F 7、.x、 V,ACOy ($Vr;I N、PMT LT L HACO9rVr. MBts0 3V ,ACOyEB+sVr, T L 2?V, ! 2 3?V,Y
4、 3 1. I N= 、T L V# =sYVr3ACOy, ( s?VH b ;(3ACOy, BT L 、 =、/ 、Y) = (si$sVr; = 8?V 3ACOy$isVrT 17.3. 2 ACOyVr+s ACSACOy ( yEB,yVrZ 9i“s,1VC 2Vr+ss、 H bVrs、 c Jr s.Y s/ VR-ACO1yVr,K .YY c , e cVr, qx2d =, h、ACO mRNA A/,70 A 6 18-19 ; %T LACOyT LFVrF( =T =TF)Vr K, =T Q 20 ; =TRACO1 3 s 3FVr, TrACO2=?V (
5、Vr,7TrACO35 =Vr 17. 2Vrs y! 2Vr ,= VrrTz.7 3 2 ,T L (Vr,0FVrK =T T h 20.,C(CmACO1 0726bp zGUSyVr, %I N= 812h,CmACO1yB“ V$ y ,Vr HW A$21.yVrV ,ACOy c is,7 OJr 9i A197 2 :ACC yZs.C(ACOyEB,CmACO1 T LB “ 7C c y 16.PT L M , “WA-ACS1mRNA 9F,Y 3 y 4ir, $ ,N HMa-ACO1mRNA 5 y 6, y /;7T L - ?_ACO 9 4,i B 22.
6、% WY db s v, c 9,ACOymRNA c vs, ACOyVr Jr , V ? Y 3Fs1yB,ACOyVr V ?Jr x y0 e 20.4 eY 3yY 3?、 Fh、 F I1T,y7 pYV eY 3 1 3 EE.Qly/ B“1 , Qly/ , |+yDNA Q_ B 0 , , P y,y ? 3ymRNARNA -QlRNA, T PMmRNA vv, PyT ss .Qly/ yVr# ? e4 rZE, H9 7B B y r、B o.|pTOM13 (cDNA)Qly T I N,yY s, s = T LsY 68%87%,YV1 0B T LY9
7、7%$,I NT L H 7 SM HWs,Mh,i O i H ? FV . PictonQlRNA/ MACC y,yI NT LY1vF/ 90%, lT L3 ?M,7vF7d,i OyT LBM,iT L *“V * y, iHq/ Vb130d;y= 9A,I N = XVC ,7yM= z.|ACC yQl,yFF 7dY 1vF64%83%, R1yF TM ,YACC / 50% 75%.N, ACC yQlRNA/ B .Y db,V7 P 9 |.XQlACC yQlACC y:P F、 、C(、 . 、?、 %) 23-28.QlACC y% =Vr,“ ?T L 、=
8、 , ?4 F I. Cooper?CQlACC yI NT L A Fh )(Colletotrichuium gloeosporioides) ? (v25) 29.MaSong|INQlACC y, 8 =Y 3,7 O A4 F V 3 ? 25.8 ,Y y y X H、Vr# e , Y 3 r# e 1il. Y yZ | VZ,1Y F IZ TT .198Z 2v1 S 2006 M ID: 1 YANG S F, HOFFMAN N E. Ethylene biosynthesis and its regulation in higherplants J. Ann. Rev
9、. PlantPhysio.l, 1984, 35(1):155-189. 2 ADAMSDO, YANG SF. Ethylenebiosynthesis:identificationof1-aminocyclobutane-1-carboxylicacidasan in-termediate in theconversion ofmethionine to ethylene J. Proc. Nat.l Acad. Sc.i USA, 1979, 76(1):170-174. 3 MATHOOKO FM. Regulation ofethylenebiosynthesis in highe
10、rplants by carbon dioxide J. Postharv. Bio.lTechno.l, 1996, 7(1):1-26. 4 YU BY, ADAMSD O, YANG SF. 1-aminocyclobutane-1-carboxylate synthase, akey enzymein ethylenebio-synthesis J. Arch. Biochem.Biophys. , 1979, 198(1):280-286. 5 GRIERSON D, SLATER S, SPERIS I. TheappearanceofpolygalacturonasemRNA i
11、n tomatoes:oneofaseriesofchanges in gene repression duringdevelopmentand ripening J. Planta, 1985, 163(1):263-271. 6 SLATER A, MAUNDERM J, EDWARDSK. Isolationand characterizationofcDNA clonesfortomatopolygalac-turonaseand otherripening-related proteins J. PlantMo.lBio.l, 1985, 5(1):137-147. 7 SMITH
12、C JS, SLATERA, GRIERSON D. Rapid appearanceofanmRNA correlatedwithethylene synthesisen-codingaprotein ofmolecularweight3500 J. Planta, 1986, 168(1):94-100. 8 HAM ILTON A J, LYCETT GW, GRIERSON D. Antisensegene that inhibits synthesisof thehormoneethylenein transgenicplan.t Nature J. 1990, 346(5):284
13、-287. 9 HAMILTON A J, BOUZAYEN N I, GRIERSON D. Identification of a tomatogene forthe ethylene-forming en-zymeby expression in yeast J. Proc.Nat.lAcad. Sc.i USA, 1991, 88(8):7434-7437. 10 SPANU P, REINHARDT D, BOLLER T. Analysis and cloning of the ethylene-forming enzyme from tomatobyfunctionalexpre
14、ssion of itsmRNA inX enopus laevis oocytes J. Embo. J. , 1991, 10(6):2007-2013. 11 DEIKMAN J, KLINER, FISCHER R L. Organization ofripeningandethyleneregulationsinafruit-specificpro-moterfrom tomato J. PlantPhysio.l, 1992, 100(3):2031-2017. 12 MELLER Y, SESSA G, EYAL Y. DNA-protein interactions on ci
15、s-DNA elementsessentialforethyleneregula-tion J. Plan.tMo.lBio.l, 1993, 23(3):453-463. 13 BARRY JP, CHRISTOPHERG D, KEVINM D. Differentialexpression of two1-aminocyclopropane-1-carbox-ylicacidoxidasegenes inBroccoliafterharvest J. PlantPhysio.l, 1995, 108(2):651-657. 14 BALAGUEC, WALSON CF, TURNERA
16、J, eta.l Isolationofaripeningandwound-induced cDNA from Cucu-m ism elon L. encodingaproteinwith homology to the ethylene formingenzyme J. European Journal ofBio-chemistry, 1993, 212(1):27-34. 15 BARRY CS, BLUMEB, BOUZAYENM. Differentialexpressionof1-aminocyclopropane1-carboxylicacid oxi-dasegenes fa
17、mily of tomato J. PlantJ. , 1996, 9(1):525-535. 16 LASSERREE, BOUQUIN T, HERNANDEZ JA. Structureand expression of threegenesencodingACC oxidasehomologs frommelon(Cucum ismelon L. ) J. Mo.lGen.Gene.t, 1996, 251(1):81-90. 17 DONALD A, HUNTER, SANG D Y. Expression of1-aminocyclopropane-1-carboxylateoxi
18、dase during leaf on-togeny inwhiteclover J. PlantPhysio.l, 1999, 120(1):131-141. 18 EON S J, JAEH L, JONGA P. Temporalandspatialregulationoftheexpressionof1-aminocyclopropane-1-car-boxylateoxidasebyethylene inmungbean J. PhysiologiaPlantarum, 1999, 105(1):132-140. 19fI 8,Z, g. qx I YACC yVr J., 1996
19、, 3(5):375-378. 20 %T LFACC yVrs J.S S/ v,199 2 :ACC yZ2001, 31(2):235-240. 21 LASSERREE, GORDARD F, BOUQUIN JA, eta.l Differentialactivation of twoACC oxidasegenepromotersfrommelonduringplantdevelopmentand in response topathogen attack J. Molecular GeneralGenetics,1997, 256(1):211-222. 22 LIU X J,
20、SHIOM IS J, AKIRA N. Characterization ofethylenebiosynthesisassociatedwith ripeninginbananafruit J. PlantPhysio.l, 1999, 121(7):1257-1265. 23 SAVIN KW, BAUDINETTE SC, GRAHAM M W. AntisenseACC oxidaseRNA delay carnationpetal senes-cence J. Hortscience, 1995, 30(5):970-972. 24 PUA EC, LEE JEE. Enhance
21、ddenovo shootmorphogenesis invitroby expression ofantiense1-aminocyclo-propane-1-carboxylateoxidasegene in transgenicmustard plants J. Planta (Heidelberg), 1995, 196(1):69-76. 25 MA QH, SONG Y R. Expression of tomato antisenseACC synthase gene in transgenic tomato and its role inshoot formation J. A
22、ctaBotanicaSinica, 1997, 39(11):1047-1052. 26 GUISM, BOTONDIR, BEN AM. Ripening-associated biochemical traits of cantaloupe charentaismelonsex-pressing an antisenseACC oxidase transgene J. J. Amer. Soc.Hor.t Sci, 1997, 122(6):748-751. 27 CUSHMAN K E. Theroleofethylene in thedevelopmentofconstant-lig
23、htinjuryofpotato and tomato J. J.Amer. Soc.Hor.t Sc.i , 1998, 123(2):239-245. 28 WOODSONW R, PARK K Y, DRORY A. Expressionofethylenebiosyntheticpathway transcripts insenescingcarnation flowers J. PlantPhysio.l, 1992, 99(2):526-532. 29 COOPERW, BOUZAYENM, HAM ILTON A. Useof transgenicplants to study
24、the roleofethyleneand polyga-lacturonase during infection of tomato fruitby Colletotrichum gloeosporioides J. PlantPathology(Oxford),1998, 47(3):308-316.Review onResearchingAdvance inACCOxidaseGenesCHEN Yin-hua1, 2,HUANGWei3, WANGHai1(1. College ofLife Science and Agriculture, Hainan University, Hai
25、kou570228;2. College ofHorticulture Forestry HuazhongAgricultural University, W uhan 430070;3. Division of Teaching Adm inistration, Hainan University, Haikou570228, China)Abstract:Being one of five phytohormones for plants, ethylene plays an important role in plantgrowth. Theprocessofethylenebiosyn
26、thesis inplantswascontrolledbyACC compoundandACC oxi-dase. Composeamountsofethylenewereeffectively coordinated throughcoordinating theirexpressionof these two speed-controlledenzymes. Thispapermakesa review focusedon theprogress incloningofACC oxidasegenes and theirexpressioncontrol, and theiruse in the future.Keywords:ethylene;ACC oxidase;genecloning;promoter200Z 2v1 S 2006 M
