1、ISSNnull 1007null7626CNnull 11null3870nullQ S 3 s 0 3 Chinese Journal of Biochemistry and Molecular Biology2006 M 1222(12):931 934null综述nullB e 1 (Perilipin)张利红, null 张立杰, null 杨公社*(西北农林科技大学动物脂肪沉积与肌肉发育实验室,陕西杨凌null 712100)K 1 null 围脂滴蛋白(perilipin)是脂滴相关蛋白家族的核心成员之一,是定位于脂滴表面的高磷酸化的蛋白,对脂肪组织中甘油三酯的代谢有双重调节作用
2、,既可通过阻止脂肪酶接近脂滴降低基础状态下的脂解,又可促进激素刺激的脂肪分解. Perilipin在脂代谢中发挥重要作用,其表达调控可能与肥胖及其相关代谢疾病如糖尿病a胰岛素抵抗等有重要关系.本文主要介绍了perilipin的发现a命名a结构特征以及激素和转录因子对perilipin的调控,并阐述了其与相关脂肪酶间的相互作用.目前的研究主要集中于围脂滴蛋白(perilipin)和激素敏感脂肪酶(HSL)之间,与新近发现的脂肪酶脂肪三酰甘油脂酶(ATGL)的相互作用则有待于进一步研究.1 o M null 围脂滴蛋白;激素敏感脂酶;脂肪三酰甘油脂酶;脂肪分解 m s | null Q513+ n
3、ull3Perilipin:OneImportant Protein in Lipolysis RegulationZHANG LinullHong, ZHANG LinullJie, YANG GongnullShe*( Laboratory of Animal Fat Deposition and Muscle Development, Northwest A hormonenullsensitivenulllipase; adiposenulltriglyceridenulllipase; lipolysisl : 2006null03null20, l : 2006null05null
4、25S E $ ? Z 9 ( 973) “ ( No: 2004CB117506)* “ null Tel: ( 029) 87091017, Enullmail: gsyang999 yahoo. com. cnReceived:March 20, 2006; Accepted:May 25, 2006Supported by Major State Basic Research Development Progam of China( 973Program) (No: 2004CB117506)* Corresponding authorTel: ( 029) 87091017,Fax:
5、 ( 029) 87092430Enullmail: gsyang999 yahoo. com. cnnull null 8 = ? Q % , Q e V TG o d b , b 8 = F ? 1 . , Q 1 PKA (HSL) . M V , 1 A % = V e , V $ (perilipin) HSL A . s 1 T , # M .1null ? C a # 20 W 80 M v p % s ? C B V $ PKA , $ V , # 1 . M 1 E B . E B M , y 9 M , V ? B y . E B 1 (perilipin), % s M
6、1 ( adipocyte differentiationnullrelatedprotein, ADRP) M T ( tailnullinteractingprotein,TIPnull47) v F , # PAT EB ,N caveolinsaS3null12 o . 8 , Plin y I , Plin y 15q26, 5 7| 8 , V | s AaBaCaD 4 . 4 B N , 1 + s C , A K , 57 kD, % % ? 3 % ( V r ; B (46 kD) 2 % V r ; C(38 kD)aD(26 kD)5 % ? 3 % V r . “
7、- BaCaD , 1“ A .Moore 2 V , ,7 i .l y I 517 , A 25% 3 u , H1aH2aH3 1 u , YV T P V 3 . Subramanian 4? C , 1 2 E ;7 H H1 H2, _ T 5 h . H1aH2aH3 H , T M E s . 3 H i O u N T null H , _ T h .2null ?Brasaemle 5 * ? . V , 3T3nullL1 8 % “ V r . v % B “ , 8 % ADRP7 perilipin. perilipin9 ADRP 5 v ,V + , E . $
8、 / , perilipin V T 3 p E V , P TG $ ,7 O / , V $ PKA , 7 PV V , N H V O .Tansey 6 L , N PKA l M d H 5 P PKAO T h .7 PKA , perilipin q r 87%,PKA V P 4 7 .Brasaemle 2000 M 5 , % V V rperilipin, P / , TG v F M 1 6 6 30 . 6 V 7 ,perilipinV V r V P % ? “ ,V 7 4 %? i , V n % ? 1 T ,# w ? 3 V ? ? 1 T . Tan
9、sey 8 ,M ! H q / , “ ( perinullnullnull ) F v 7 3 (peri+ null+ ) 30%. “ % V C $ q , V 7 perilipin “ T , H V C O T / A ,V K v ? A T .N , “ t i ,8 t , q , 0 O F 6 t . J H , “ V J g . CastronullChavez 9 ,perilipin “ s Y 270 543 c A / , x y null , , 0 . , / y , ( “ g V . N , “ g F 3 o V ? g 4 B. Z _ .3n
10、ull HSL “ - K .d HSLi . 10 , V T 3 p , $ = HSL ; O nullnull / F s 8 4 J / F T / ,Plin $ PKA , 7 M V T Z T , P HSLV A V 7 S , V A ,N ! p null ( M T Z T V Fig. 1VU ) . V , i d 8 = V , ,HSL v % + B 11, 12 ,i O , Y V i T ? C T 9 V 1i % 4 13 , V 5 ! p null V V perilipin $ HSL_ T , ? b # d V ( ( L X V ).M
11、oore 2 V ,8 = 4 932 S 3 s 0 3 22 HSL perilipin F . Plin K ? T . “ M - 9 ,HSL ? v / , Mv % - v M 1 q A , 6 - 8 12 - v J 24 h, T - % A h 12 . Tansey 6 ,$ % , HSL i f / ,PKA T ? 3 d (v 0null5 1 h), HSL F 7 S ,i 9 F q .Fig.1null Current modelof adipocyte lipolytic stimulation14null null N ,K Zimmermann
12、15 ? C i s , (ATGL), V , 1 % V r , V C + s , 1 B o , i 1 . “ ,ATGL 75% . TG V , = M 1 , q . NV , B K . V 16 , ATGL $ ? 1 T , 7 $ / , p T , $ .y N ,w ATGL W V ? i M T B .4null e T Arimura 17 , s % ,Plin y V r 1 PPARnullnull2 e ,PPARnullnull(V 8 9 s 8 nullnull) % s 1 o e y 0 , 3T3nullL1 % F PPARnullnu
13、ll 4 V A perilipin y V r . Nagai 18 , v Plin 0 B ? PPAR s q (PPRE),i PPARnullnullY V 0 u PPRE v Plin y c e T , c y 0 e Plin y n .1998 M Souza 19 TNFnull O 3T3nullL1 % ,? C % = V r / ,7 Y V F h P perilipinV V r H 5 E TNFnull . 4 U TNFnull e V ? B , Y V V L C .Mooney 20 V , J / F O / , v v F , O ) , T
14、 5 $ v d ( 6 2 3 ) ,V G T .5null l 8 , o y V ? “ O F % h 1 , M 1 E B # ? 1 e T , Y V 1 o HSL M T _ , $ ATGL 9 V ? e T , 6 B ? , 8 T “ - , / B .Kern 21 _ V r g A M 1 ( r= 0null55), O y s PPARnullnull e ,# w 1 o933 12 f :B e 1 ( Perilipin) nullT V ? g # M 1 % h ? 3 1 , V B 8 s s ,O F 7 B B Z _ ,K 0 7
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