北京 雷根 生 物技 术有 限公 司 SDS Triton SDS NP-40 SDS (SDS Lysis Buffer) NP-40 RIPA ( ) RIPA ( ) Western IP Western (ChIP) SDS Lysis Buffer Tris-HCl NaCl SDS ( ) ( ) 1 SDS Lysis Buffe PMSF 1mM 2 3 6 150 250 l PMSF SDS Lysis Buffer CHIP, 4 4 ( ) 5 Western (ChIP) ( ) 1 SDS Lysis Buffer PMSF 1mM 2 3 6 150 250 l PMSF SDS Lysis Buffer 4 4 ( ) 5 Western (ChIP) ( ) PS0015 Storage SDS Lysis Buffer 100ml -20 PMSF(100mM) 1.5ml -20 1 北京 雷根 生 物技 术有 限公 司 1 SDS Lysis Buffer PMSF 1mM 2 3 1 2min 4 20mg 150 250 l PMSF 4 5 ( ) 6 Western (ChIP) 1 2 3 50-100 / 4 Vortex 5 SDS Lysis Buffer 6 4