1、,Lectures on Writing Medical Paper Abstracts in English,第五讲SCI 收录我国三种医学杂志英文摘要赏析,SCI 是美国科学引文索引 （Science Citation Index）的缩写。由于SCI 数据库具有学科全面、学术影响大、覆盖国家广泛等特点，因而被其收录检索的期刊和论文分别被称作国际主流期刊和国际主流科学。刊物或论文被SCI 收录和引用在一定程度上反映了该刊物或论文具有较高的学术水平和较大的国际影响力。 我国目前被SCI 收录的科技期刊共60种，而临床医学类只有中华医学杂志（英文版）、中国药理学报和世界胃肠病杂志3种。,一、中国
2、药理学报 （Acta Pharmacologica Sinica) Sample 1Acta Pharmacologica Sinica 2007 February; 28 (2): 238-245Effect of genistein on mouse blastocyst development in vitro,Abstract Aim: 1)To examine the cytotoxic effects of genistein, an isoflavone compound, on early postimplantation embryonic development in vi
3、tro. Methods:2) Mouse blastocysts were incubated in medium with or without genistein (25 or 50 mol/L) or daidzein (50 mol/L) for 24 h. 3)Cell proliferation and growth was investigated by dual differential staining, 4)apoptosis was analyzed by terminal deoxynucleotidyl transferase-mediated dUTP nick-
4、end labeling (TUNEL) assay, and 5) apoptotic or necrotic cells were visualized by Annexin-V and propidium iodide (PI) staining. 6)Implantation and postimplantation development of embryos were measured by in vitro development analysis,Results: 7)TUNEL staining and Annexin-V/PI staining showed that ge
5、nistein dose-dependently increased apoptosis in mouse blastocysts, while daidzein, another soy isoflavone, had no such effect. 8)The pretreatment of the blastocysts with genistein caused fewer cells than the control group and this effect was primary in the inner cell mass. 9)The genistein-pretreated
6、 blastocysts showed normal levels of implantation on culture dishes in vitro, but significantly fewer genistein-pretreated embryos reached the later stages of embryonic development versus the controls, with many of the former embryos dying at relatively early stages of development. In addition,10) g
7、enistein treatment decreased the development of morulas into blastocysts, and dietary genistein was found to induce cell apoptosis and decrease cell proliferation in an animal assay model of embryogenesis.,为主-动-宾-补(SVOC)结构的被动语态,Conclusions: 11)Our results collectively indicate that genistein treatme
8、nt of mouse blastocysts induces apoptosis, decreases cell numbers, retards early postimplantation blastocyst development, and increases early-stage blastocyst death in vitro, while dietary genistein appears to negatively affect mouse embryonic development in vivo by inducing cell apoptosis and inhib
9、iting cell proliferation.12) These novel findings provide important new insights into the effect of genistein on mouse blastocysts.,Sample 2 Effect of nicotinamide on newly diagnosed type 1 diabetic childrenAbstractAim: To determine whether a low dose of nicotinamide (NA) therapy for pediatric patie
10、nts with type 1 diabetes, initiated within the first 24 h of diagnosis, prolongs the “honeymoon“ period and lowers their insulin requirements.,Methods: All children (n=66) with newly diagnosed type 1 diabetes admitted to Salmaniya Medical Complex between 1998 and 2000, received NA 1-2mg/kg per day,
11、in addition to sc insulin bid. The patients were followed for 24 months (NA group). Findings in this group were compared with records from a similarly diagnosed control group (n=37), who were admitted to the same hospital between 1995 and 1997 and did not receive NA treatment. The insulin dose per k
12、g bodyweight required at baseline and at 3-monthly intervals up to 2 years after diagnosis was determined.,Results: At baseline, the two groups did not differ with respect to age, ethnic background, weight, insulin dose per kg bodyweight or glucose levels on admission. However, NA group had lower in
13、sulin requirements than control group at each 3-month interval up to 2 years after diagnosis. Conclusion: Our study results suggest that even low doses of oral NA given to children with newly diagnosed type 1 diabetes may reduce insulin requirements and prolong the “honeymoon“ period.,范文赏析 中国药理学报 （A
14、cta Pharmacologica Sinica)的英文 摘要具有以下特点： 1） 采用结构式英文摘要 2）多用一般过去时和被动语态 3） 字数120246，平均160字 4） 说明研究目的一般采用不定式 5） 文题均为名词短语，包括3种形式：名词+介词短语，如of, in.名词前也可加修饰词；名词+分词短语，现在分词或过去分词；名词+不定式。,6）常用句型分别为：表示目的：To investigate, To study, To explore, To surveyand assess表示方法：was proposes, were randomly divided into, was de
15、signed by, were tested by, was measured by, was used to detect 表示结果：was determined, was decreased, demonstrated, showed, went up to, was significantly higher表示比较：of A was / were higher than that / those of BA has some similarities with B, There was no significant difference between A and BNonsignifi
16、cant difference was found between A andB (in),二、中华医学杂志英文版 (Chinese Medical Journal, CMJ)中华医学杂志英文版是中华医学会主办的综合性医学学术期刊，是中国历史最悠久的医学杂志。该杂志重点报道我国医学各学科最新进展和高水平科研成果，以及临床各科诊疗经验，是我国医学交流的重要窗口。目前该刊物被国内外10个重要生物医学数据库、检索系统和文摘期刊收录。Chinese Medical Journal 全球发行，作为医学会会刊和全世界50余个国家和地区建立交换项目。,Sample 1Calpain I inhibition
17、 prevents atrial structural remodeling in a canine model with atrial fibrillationXUE Hong-jie, LI Wei-min, LI Yue, GONG Yong-tai, YANG Bao-feng, JIN Cheng-luo, SHENG Li, CHU Shan, ZHANG Li, SHAN Hong-bo, LIU Jie Keywords: atrial fibrillation structural remodeling calpain inhibitorN-acetyl-leu-leu-me
18、tAbstract: Background Atrial fibrillation (AF) is accompanied by atrial structural remodeling. Calpain activity is induced during AF. To test a causal relationship between calpain activation and atrial structural changes, N-acetyl-Leu-Leu-Met (ALLM), a calpain inhibitor, was utilized in a canine AF
19、model.,Methods Fifteen dogs were randomly divided into 3 groups: sham-operated group, control group and calpain inhibitor group; each with 5 dogs. Sustained AF was induced by rapid right atriumpacing at 600 beats per minute for 3 weeks. ALLM was administered at a dosage of 1.0 mgkg-1d-1 in the calpa
20、in inhibitor group. Three weeks later, the proteolysis, protein expression of TnT and myosin, calpain I localization and expression and structural changes were examined in left atrial free walls, right atrial free walls and the interatrial septum respectively. Atrial size and contractile function we
21、re also measured by echocardiography.,Results Long-term rapid atrial pacing induced marked structural changes such as enlarged atrial volume, myolysis, degradation of TnT and myosin, accumulation of glycogen and changes in mitochondrial shape and size, which were paralleled by an increase in calpain
22、 activity. The positive correlation between calpain activity and the degree of myolysis (rs=0.90 961, P0.0001) was demonstrated. In addition to structural abnormalities, pacing-induced atrial contractile dysfunction was observed in this study. The pacing-induced atrial structural alterations and los
23、s of contractility were partially prevented by the calpain inhibitor ALLM.,Conclusions Activation of calpain represents key features in the progression towards overt structural remodeling. Calpain inhibitor, ALLM, suppressed the increased calpain activity and reversed structural remodeling caused by
24、 sustained atrial fibrillation in the present model. Calpain inhibition may therefore provide a possibility for therapeutic intervention in AF.,三、World J Gastroenterol （世界胃肠病学杂志）世界胃肠病学杂志（World Journal of Gastroenterology, WJG) 已被SCI、IM、CA、EM等国际检索系统收录。WJG主要报道食管癌、胃癌、肝癌、大肠癌、病毒性肝炎等原始论文，同时还设置文献综述、基础研究和临床
25、研究栏目，对促进国内外胃肠病学信息的交流及学科的发展有着重大的意义。,Sample 1Correlation of ATP7B genotype with phenotype in Chinese patients with Wilson disease.AIM: To determine the mutational characterization of P-type ATP7B gene and to explore the correlation of ATP7B genotype to phenotype in Chinese patients with Wilson disease
26、 (WD). METHODS: Seventy-five patients with WD from 72 no-kinship families, 44 males and 31 females, were enrolled in this study. The age of onset ranged from 4 to 39 years, =18 years in 72 patients. Some exons of ATP7B gene mutations were analyzed in patients with WD by using biochemical methods, po
27、lymerase chain reaction-single strand configuration polymorphism (PCR-SSCP) and DNA sequence analysis. A total of 778 coding regions were identified with restriction enzyme Msp I. The activity of Cu-ATPase was assessed by measuring inorganic phosphorus.,RESULTS: Sixty-six of 75 patients (88%) had wi
28、th hepatic manifestations, 39 of them had only hepatic manifestations, 27 patients had hepatic and neurological manifestations or other symptoms at the same time (16 patients had associated neurological manifestation, 3 patients had osteopathy, 8 patients had other symptoms). Eight of the 75 patient
29、s (10.7%) had only neurological symptoms, one patient (5 years old) had no symptom. Twelve changing patterns were detected in ATP7B gene by DNA sequencing, including seven mutations (R778L, C656X, G943D, V1140A, V1106I V1216M and 1384del17), six polymorphisms (IVS4-5t/c, A2495G, C2310G, IVS18+6c/t a
30、nd IVS20+5a/g).,R778L occurred in 49/66 patients (74%) with hepatic manifestations, homozygosis of R778L in 16 patients, heterozygosity of R778L in 33 patients. V1106I mutation of ATP7B gene occurred in 2 patients with delaying onset of clinical symptoms. Cu-ATPase activity of three patients with kn
31、own mutations (R778L/V1106I/A2495G, R778L/V1216M and R778L/R778L) were determined, and the activity of Cu-ATPase was decreased by 44.55%, 88.23% and 69.49% respectively. CONCLUSION: 1384del17bp is a novel mutation found in WD patients. R778L is the most common mutation of ATP7B gene. There is a correlation between R778L and hepatic manifestations in WD patient.,
