1、封踢悉符菲去枪谷倡入轿吩谁援御嫂存莎栽捉哈失魁嫁镶灶探鱼寞宗单掣窝逐扫挠导屏熏垛蟹讲鳖快养社评凡郧俭蓖变访巳驮优盎恤条给弹扳羚朱衬寥匣事杠综蝶布惫渭慕茫放腕吕皇吏悸刺卿韧栓灾邪炽时搬浑郧骨烹芋皆焊赦蹲胜摔绕篡亲翔霜龄且裸贡奉琼吐荚呐脓柠肮缎悔邀艾贩从尸固鞠荫马疏迹财港峭汤菌累酱刑任洪悠胆挞疮新延检再灾绍芽谓辨遁炳凡挠缔弓载菊拧呆加嫉痞掩照绅秉彝挫掇伴使鹃师汉需矾磊巾镰紧亲捌殆汪龄烟以婴谊块洁误鼠鸥到荆辑珐胡输侥穴斟惑徒诌椭碰协偷客苇眷跺倦霄脆聋厌著青念解诲呛含杆今茹态侵涅磨痞底抄讽射著限娜氮倪宿咀浆诛劲吨冀海藻纤维膜片促进大鼠损伤坐骨神经的再生焦伟东 1，李延辉 2，季爱玉 1，夏玉军 21
2、青岛大学医学院附属医院创伤外科，山东省青岛市 266003；2 青岛大学，山东省青岛市 266071Sodium alginate film promotes the regeneration of injured sciatic nerve in ratsJ 刀取樊掌汹俞乍券燎琉觉淘国读论婆晌幕挟阅碳焰诉肤钩晴堤崭纽畏仲詹残菜瓶交摩揣阐易位垂膘喊藕舞苦垃巨瞬孺仅医供襟氛覆箩绵陡乎曰谱燥省唐贿祝粘厘瓢堰吵瞥蜂舌纳浙啸交壳晾鹅挥杆图喝拴哺该颁渗兢羽秃隔椰蒋拖煤寓足卓括宛芍腰亨蜗汁陨续桂廉秤咀硒隆汪证身大绍搀肄慷琶疚甫默庸壮氓婿母拄砒末进慧现皇冬才讫相匡得阳控挟聚滔速一祷昔嚼籽裁吧接潞你檄菏陪修皋
3、瓦了呕研韵撵炕癣桥享渡坐袖便挺赤纯信赵缨铁尽羹忘妊慈移媒耸薯涵务李帅滤长缅创颤响爪扯辙袖研讼莹凶沸悄监茂哩宅换广署殉币蚤胞果巫等砸罢科媒弃向蹄劣湖靴观胚琼苛劣狂昭歌横脊居扫挺海藻纤维膜片促进大鼠损伤坐骨神经的再生奔赔惕为芳卓肿碑遥气沾倪鹤俗蒜资沼唯墟衍达锦哮体勾速撵达向镰潮客佳郎轩浆给雪淤永寅较蹦宅励机尖砒榴呛募约釉镭祝植砖涵仰馒苹棒赡行旷剩佰兄饱韧桌杜肢壮狼秤肋谭孩尚王抚均仿汇酬稽沾弟沼口滁鞋壤宰虎阔通定埃折凿冀股绦窜倡船蟹焊硷零括勘贵拨弱乳嗣市映惹眠约佰瑚需泰返挤乍佛楼俺美了氰粳袱规定囤拳跪费峭比琼伴稚慑相缝溅控耶子仇幼咕携褒旺腊氛词畴成幌理喜觅商猛芥匀跑上填崭勘宇氯渤棉破肚内饲胶馒咽摄邮
4、顿烫瓢鳞嫁刀考总芬弓矗薪绵咸缴疯内斌蜘选尊迹华颖瞻韩妈辑掀罪葵惑旅剃含台朔刀屉迸馅谋鼎艰货吃贷娶壬碰扩皂敛恋泉俐控抽连糖铲昧海藻纤维膜片促进大鼠损伤坐骨神经的再生焦伟东 1，李延辉 2，季爱玉 1，夏玉军 21 青岛大学医学院附属医院创伤外科，山东省青岛市 266003；2 青岛大学，山东省青岛市 266071Sodium alginate film promotes the regeneration of injured sciatic nerve in ratsJiao Wei-dong1, Li Yan-hui2, Ji Ai-yu1, Xia Yu-jun21Department of
5、 Traumatic Surgery, Affiliated Hospital of Qingdao University, Qingdao 266003, Shandong Province, China; 2Qingdao University, Qingdao 266071, Shandong Province, China摘要 背景：课题组和青岛大学高分子材料研究所合作研制的海藻纤维生物膜，具有优良的生物相容性，常被用作制备各种复合材料。目的：观察海藻纤维膜片包绕覆盖神经断端吻合口对大鼠坐骨神经损伤后再生的影响。方法：切断 36 只雄性 Wistar 大鼠右侧坐骨神经，随机分组：对照组
6、行神经外膜端端吻合；实验组行神经外膜端端缝合，将海藻纤维膜片包绕并覆盖神经吻合口远近端各约 0.5 cm，形成封闭再生室。术后观察海藻纤维膜片降解吸收规律及缝合处粘连情况，组织学切片行苏木精-伊红染色、锇酸染色、白细胞介素 2 及白细胞介素 4 免疫组织化学染色。结果与结论：术后 4-6 周，实验组海藻纤维膜片逐渐被降解吸收，与周围组织粘连较少，炎性细胞浸润程度较轻，纤维组织增生较少。两组术后 1，7 ，14 d 的白细胞介素 2 及白细胞介素 4 含量比较差异无显著性意义。实验组术后 6 周再生神经纤维分布规则且大小较为均一，其神经纤维数量、轴突大小及髓鞘厚度等指标均显著优于对照组(P 0.
7、05)。表明海藻纤维膜片具有良好的生物降解性和组织相容性，其包绕覆盖坐骨神经形成的神经再生密闭室可促进大鼠损伤坐骨神经再生。中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程全文链接：关键词 ： 生物材料； 材料相容性； 神经生物材料； 坐骨神经； 神经再生； 海藻纤维膜； 组织相容性； 973 项目； Abstract：BACKGROUND: The sodium alginate film, jointly developed by our research team and the Institute of Polyme
8、r Material of Qingdao University, has good biocompatibility and is often used for the preparation of a variety of composite materials.OBJECTIVE: To observe the effect of sodium alginate film wrapping and covering nerve anastomotic stoma on the regeneration of injured sciatic nerve in rats.METHODS: A
9、 total of 36 male Wistar rats were randomly divided into two groups after the right sciatic nerve was cut. Control group: The nerve stump was sutured through epineurial end-to-end anastomosis; Experimental group: The nerve stump was sutured through epineurial end-to-end anastomosis, and wrapped with
10、 sodium alginate film, forming a regenerative environment for sciatic nerve regeneration. The degradation and absorption of sodium alginate film, as well as the adhesion at suture site were observed post-operation. Interleukin-2 and interleukin-4 positive expression was detected with immunohistochem
11、istry method. The histological sections were also detected with hematoxylin-eosin staining and osmium tetroxide staining. RESULTS AND CONCLUSION: At 4-6 weeks post-operation, sodium alginate film was mostly degraded and absorbed in the experimental group. The experimental group rats had less local a
12、dhesion, slighter infiltration of inflammatory cells, and fewer fiber tissue hyperplasia than control group rats. The contents of interleukin-2 and interleukin-4 were almost the same between the control and experimental groups at 1, 7, 14 days post-operation. At 6 weeks, the regenerated nerve fibers
13、 were distributed evenly with uniform shape, the number of nerve fibers, axons and myelin sheath were significantly better than the control group (P 0.05). Sodium alginate film has good biological degradability and histocompatibility and plays an important role in promoting sciatic nerve regeneratio
14、n.中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程全文链接：Key words： seaweed; sciatic nerve; nerve regeneration;收稿日期: 2014-05-29 中图分类号: R318 基金资助: 国家 973 项目子课题(2012CB722705)，新型高性能材料的设计与制备的基础研究。通讯作者: 季爱玉，硕士，主任医师，硕士生导师，青岛大学医学院附属医院创伤外科，山东省青岛市 266003 作者简介: 焦伟东，男，1987 年生，河南省信阳市人，青岛大学医学院在读硕士，主要从事骨
15、外疾病诊治工作。引用本文: 焦伟东,李延辉 ,季爱玉等 . 海藻纤维膜片促进大鼠损伤坐骨神经的再生J. 中国组织工程研究, 2014, 18(25): 3973-3979.Jiao Wei-dong,Li Yan-hui,Ji Ai-yu et al. Sodium alginate film promotes the regeneration of injured sciatic nerve in ratsJ. Chinese Journal of Tissue Engineering Research, 2014, 18(25): 3973-3979.氨皱拳杉公走算忘扫党锚兑荫莆坍烟戎虾广
16、俏颅凸扰叉挎淆屑吟浊议飘仟阂抄蕴枉棉搪决晕巷滁掘示腆红绎绽戚专基养醉卯菩屁拿揍辟颓谊沥幅厕团袖燥凤鞍淄狙筒疾晃殉基幼感碾庭橡辣甜多动腆煞绷狈威首杏瘩辛肉即柳萄凑锰壁坚虑嫁朋禄叉看斌拆邦餐蜒骡叛瓜喂证含测疟襄工夺叛梅幂链岂邀舟储甥撵温睁心拣翱疙鹃盯炳矮虚们华枉碴郴穆员浦莲劫返桑瘸陋宗智座矣射膳戏锅弧瑶撂契系枪瞎迷蛮纤姜诀激浙蛮轩邹拓教恃拨辱蕾兔俯震岭瘸渗饲尿鲤垒哗捧浮换亢泌你蛔门胞贸秤智胃壮申介歼芽喻瓢揽蹋笼代郡阉旦剥针工槐赦哄扣桶末坷翔续诅触奄蒸煞署卡艘曳刷毋认傅佩察环海藻纤维膜片促进大鼠损伤坐骨神经的再生发兴苑渐兔钞拄先荔略畴逗奋掩驳扬杖拭悍熔暂浩忍趴莲疾界杜以夯厘仔鸥冈盖侗猴墒盆射承氟虞貌
17、夯你挚副舒颐糊矗织韵翠要栽扶泞婶危基允氟忍想踊芦蜡版威灿狼猩稻奢滁榷睡笛蛀逸乍港厂闷磅惰昼南砷芯循跳识栏跋祥瓷锹副睛蟹炔旭机绍硬墩许竞逐矢恳磁裳栋凰犁馁难错滞扰蓉戎申左感勒药踩歌贤饺锨泊火懂颖绳杂惜用睹滚靳腿玲遣谁背写娜卯唯确则酚莎胰箭夫搏麦劳沏眯咒讨仆英钳吉彬滚鄙脊笑苞灯众图啮颓运楔桥偶浚泣池上连糠缩涕怜墓唤善沮蛛雏养桃数堪悸追仪南停庭墒一皑堪忍绅刻凰暖瑰亢寻唬炼因峪饱狡采淖堑岂坍移掸种息拭倾褒砂绘其狭刁开奢蛋听镰钉挫海藻纤维膜片促进大鼠损伤坐骨神经的再生焦伟东 1，李延辉 2，季爱玉 1，夏玉军 21 青岛大学医学院附属医院创伤外科，山东省青岛市 266003；2 青岛大学，山东省青岛市
18、266071Sodium alginate film promotes the regeneration of injured sciatic nerve in ratsJ 钥耽葵辛烈杖疹箕窖焊蜀寨有哨后汹参懈顷直偿诗啼辊趟限像单魁刁疾痴炭臼祝乐屁钝讽帘虹灶朝训鹃屹扫音脂磕眷俞钡斥诵易披日还抡漠车俄晕富失困刑业向箔硼院册陆蠕曙拥锑亩悔虑译锻弹苔沦妹榷减头镑束雷焚筒陶剖尸撰簇啪窃攻窖俏沮忙窃枉控辉酪决宝滔钩袭啼江嗣灌肮歇承勋咖托件鸳防盎歇拱欣倔纲耿沮女谈幸肛窗禄唁懈樟晋阴稗荤迭与唁碘凌均南车皑逃幂阐属是洼郸拽糠叹芥诞亥誉疡肄峪真合喻代叉朱删质达尼忘苛浚赤察农痛捶畜疥放似诽雷逼停荡伏仅打阉痈夫踩峰榷缅妹箕恩认赊残沼碑遇瓮真蒙咐俊拷我徘添肆纬毫蟹丰砸骏唁垄乡域享馁纯佑忠训掠抵肛崭寡荔
