1、微生物学通报 NOV 20, 2010, 37(11): 16791684 Microbiology China 2010 by Institute of Microbiology, CAS 基金项目： SE 8 e S/v “ (No. 2009ZX07211-009); H 3C , i| “ANAMMOX”, Van de Graaf YVv L ANAMMOX B 3V , i15N S: NO2 ANAMMOX 1o0 s8 , 7 - NO3, i4 V ?o m 1 UbyN ANAMMOX % ) Hq/ NO20 s8 , | 3Vb (NH2OH) (N2H4) VW ,
2、 K V ?0 s8b NO2 3 , BV 4 1 01b 图 1 厌氧氨氧化可能的代谢途径2Fig. 1 Possible metabolic pathway for anarobic ammonium oxidation2Strous2 SBR Q “ % )V , 9 4 Q V ?9QZ T , n T (1)b 1NH4+ 1.32NO2+ 0.066HCO3+ 0.13H+ 1.02N2+ 0.26NO3+ 0.066CH2O0.5N0.15+ 2.03H2O (1)2 厌氧氨氧化细菌的分离鉴定 % ) 3d , 9 HWr 1120 d, C 3s / 4 B“ )b “ -
3、YVQ HW“ ! , 1 )b Strous2 SBRQ H 1 M“ ! % ) 70%“ !bk ) B ?5 o f|% ) , 0A/VC?5 3+bYV 16S rDNAk , N% ) ) “ (Plancetomycetales)B s , $ Candiatus Brocadia anammoxidansb “ -Q ?C % ) 5 , 93(m 2), ( 图 2 厌氧氨氧化细菌系统发育树3Fig. 2 Phylogenetic tree of anammox bacteria3la : ) Z 1681 http:/ ) “ (Planctomycetales), Ca
4、ndidatus Kuenenia stuttgartiensis yFT9X4b Hu 5?CB L )% )9 B , i“ ?% )iB ) “% )b 3 厌氧氨氧化的影响因素 3.1 温度对厌氧氨氧化的影响 Y% ) 31y Bb 6s LTV Q aR 030C; 40C H , % )$ /b Dosta 7 18C/ SBRQ , /9V ? b 3.2 溶解氧对厌氧氨氧化的影响 Y QB1yb $ L QbStrous8YV 2%a1%a0.5% 0%( b ) i/ L?C , i 2%a1% 0.5% LF , ?C NH4+$ , 0% i LF , f , C NH4
5、+ NO2 Hh b d , ? Hq/ , iv 2 mol/L 3 V ITb 3.3 pH 对厌氧氨氧化的影响 pH9 VB1Yy bS = pH Y bD Q aR pH S 6.78.3, KvQ qC 8.0Pb 6?C , pHV 6.0 6 7.5 H , qv4 ; pH ? 6 9.5 H , q/bC R K a pH 7.88.0Wb 3.4 底物浓度对厌氧氨氧化的影响 1 b , ib %TbStrous9b 1 g/L f / 3T , b V0.1 g/L, Q $bb T VYV_Q F Wr“bf 10 b i YbTV b i4 F y ANAMMOX Q
6、q , NO2N=118.4 mg/L H , qrK ; B4 NO2N i ANAMMOX 3 AT , i4 aR NO2N : NH4+N 1.3 : 1b f 11 T 3Q i Y H , b “ i 280 mg/Lb3 | TQ H , b “ i320 mg/LPbyN i Yi , VYVM T TMQ4 V 0 s ib 3.5 其他因素对厌氧氨氧化的影响 QHqM 3Q7 N YbS =a pHy Y H , 9 a Tb Kang 12?C , COD 0500 mg/LS = H , “ COD 6b “ q 6b Jetten 13 % )“ !V?C Q 3 A
7、Y , % ) ? 0 s 1 mmol/L , i550 mmol/L H , sb 14 Ca/p1 Y , Ca/p Tbf 15F Fe3+V % ) 3 , 4 ; Zhang 16 CANON H , F NO2r4 AUSB Q r q ; h17I YbN9D , % ) ; 3 , ; ? , ? 30%50% “ q18b 4 厌氧氨氧化的启动运行 4.1 厌氧氨氧化反应器 1 Mulder 5?C 1682 微生物学通报 2010, Vol.37, No.11 http:/ , S = Hq#+ , $b % ) 3 , H , i O% i r 10101011 /mL
8、 ? zAC 2, # CT = HW Q b “ -Q 1 5a SBRaUASBaEGSBaASBRa%5a 6 TQ 3 rb Cz Ca R Ca h CaQb Ca Ca1 rA) Cb 191 z Ca R C h C 3 C r ? , TV , R C 3 CK a“ ) Cb“.d CZ T , C%/ 9$ 20b SBR 3 = as ( , V , yN “ - KQ b“W SBR , S =9 Q , i ) rTb F21 UASB , NH4+N NO2N “ q 99.9% , TN “ q9V 90%bf 22 ICQ 1 mm2 mm R Cb Wang2
9、3EGSBQ 3 = , CODCr 500 mg/L f / , CODCr“ q Vr80%bTrigo 24 3Q , “ qr 710 g/(m3d), b s “b 4.2 厌氧氨氧化试验用水 v , $ b KD 9“ q Vr 26 kg N/(m3d)25, V.d 3b “ P , M , S = 7 S Pl , i OXs Ll 02627b “ -l l , 1 ChAa CrAal # 1 rAb Joss 28? /b - ) h C bA , qr 500 g N/(m3d), 90% bQiao 29 ) 5l , 9“ qr 3.12 kg N/(m3d)bN
10、 , 9| 3 1rA) V3031b 5 厌氧氨氧化工艺 “ - 7?OLAND (K1!b -Qb , Oxygen limited au-totrophic nitrification and denitrification) , CANON (b 1! , Completely autotrophic nitrogen removal over nitrite), SHARON (Single reactor for high activity ammonia removal over nitrite) ANAMMOX b OLAND 1998 M1 H Gent v 3 3 L i
11、7? , Kb M 3Q“dbKHq/ , PbV NH4+ NO2, ?7 NO2Q NH4+ N2bWyffels 32 OLAND ) ChA ; Windey 33TV OLAND) l B Vb SHARON-ANAMMOX34 Delft v 2001 M 7?Bb 2Q = , 1Q = , % )Hq/| 3 NO2, P NH4+:NO2= 1:1 P ; 2 Q = , % ) Hq/ NO20 s8| NH4+ 3N2bSharon B X NO2N 3 , .db -QbVM , Sharon-Anammox V V Pnh 90%, CO2b h 88%, 3N2O
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