1、 1 C030316 * * * 110001 92 024-23256666 024-23256666 2003 10 B5 1 3 35 100083 * * * * * * A. B. C. D. E. F . H . A 1 2 1 C030316 2 A. B. B 15.7 2 2003 1 2004 12 A. B. * A. 1959 12 XXX B. B 210102*476 01 ( ) 011 A. B. C. A 156 A. B. C. B * 11000101 110001 A. B. C. A 024-23256666 921 X X ( ) XX ( ) XX
2、X XX ( ) 92 8 3 3 1 1 1 1 * 210102*253 * 11000101 * 210102*183 * 11000101 * 210102*182 * 11000101 DNA PCR * 210105*316 * 11000101 * 210102*171 * 11000101 PCR * 410305*451 * 11000101 * 11000101 ( ) 1 1. 2. / B A B 25 ( 1 12 () 2 DNA DNA PCR DNA DNA 20 80 90 DNA 1 1893 Nucleated RBC, NRBC NRBC 1 10 9
3、1 10 5 NRBC 90 DNA NRBC NRBC 1. 2. CD71 GPA NRBC 3 2 CD45 fluorescence-activated cell sorting, FACS magnetic-activated cell sorting, MACS 2 3. NRBC NRBC 4. NRBC 3 NRBC NRBC (4) NRBC CD45 FACS MACS 80% 87.5% 1. NRBC 2. PKU 5 100% 0% Human Leukocyte Antigen, HLA HLA A-Exon 2, A-Exon 3, B-Exon 2, B-Exo
4、n 3 DQA-Exon 2, DQA-Exon 3 PCR HLA DNA 1. Lo YMD, Wainscoat JS, Gilmer MDG, et al. Prenatal sex determination by DNA amplification from maternal peripheral blood. Lancet, 1989, 2: 1363-1365 2. Bianchi DW, Klinger KW, Vadnais TJM, et al. Development of a model system to compare cell separation method
5、s for the isolation of fetal cells from maternal blood. Prenatal Diagnosis, 1996, 16: 289-296 3. Wachtel SS, et al. Charge flow separation: qyantification of nucleated red blood cells in maternal blood during pregnancy. Prenatal Diagnosis, 1998: 18: 455-463 4. Wang TR, Chen HP, Lu Y, et al. Y specif
6、ic sequence gene analysis of single fetal nucleated erythroblasts from the peripheral blood of pregnant women. J Tongji Med Univ. 2001; 21(2): 163-5. 5. Watanabe A, Sekizawa A, Taguchi A, et al. Prenatal diagnosis of ornithine transcarbamylase deficiency by using a single nucleated erythrocyte from maternal blood. Hum Genet 1998102: 611-5 1. * 1. 2. 4 2 A-Exon 2, A-Exon 3, B-Exon 2, B-Exon 3 DQA-Exon 2, DQA-Exon 3 PAGE PCR dATP 9 6 12 2. 1 5 4. 5. 6 1. 2.
