1、生物化学与分子生物学PPT素材,可根据需要选择取舍,Metabolism of 5-fluorouracil (5-FU),5-Fluorouridine,5-FUMP,5-FdUMP,Uridine phosphorylase,5-Fluorodeoxyuridine,Thymidine phosphorylase,Orotatephosphoribosyltransferase,Thymidine kinase,5-FUTP,5-FdUTP,RNA,DNA,5-nucleotidase,5-nucleotidase,Uridine kinase,ATP,ADP,ATP,ADP,PRPP,W
2、estern Blotting,1. Resolve protein samples on SDS-PAGE,6. Incubate the blot with chemiluminescent HRP substrate and expose to film,2. Electrophoretically transfer fractionated proteins from gel onto PVDF membrane,3. Block the membrane with neutral protein (BSA or milk casein),4. Incubate the membran
3、e with primary antibody specific to target protein,5. Incubate the membrane with HRP-labeled secondary antibody specific to primary antibody,Cholesterol,Sterol Toolkit,Pregnenolon,Progesteron,Aldosteron,Cortisol,Corticosterone,Androstendione,Testosteron,Estriol,17-Estradiol,1, 25-Dihydroxycholecalci
4、ferol,Cholic acid,Covalent modification of proteinsPoly(ADP ribosyl) Polymerase (PARP),Poly(ADP ribosyl)polymerase(PARP),NAD,Nicotinamide,Poly(ADP ribosyl)polymerase(PARP),Poly(ADP ribosyl)chain elongationand branching,Nicotinamide,NAD,NAD,DNA polymerase ,DNA polymerase ,DNA polymerase ,Oligosachary
5、ltransferase,N-glycosylation pathway in ER,Mann-P-Dolicholtransferases,Flippase,Dolichol,P,P,Dolichol,P,P,Dolichol,P,P,Dolichol,P,P,GlcNAc-Ptransferase,GlcNActransferase,Dolichol,P,GDP-Mannosyl transferases,Glc-P-Dolicholtransferases,Dolichol-pyrophofphatase,Cytosol,ER,40S,60S,UTP,CTP,TMP,Pyrimidine
6、 Nucleotide Interconversions,UMP,ATP,ADP,H2O,UDP,Nucleosidemonophosphatekinase,ATP,ADP,Nucleosidediphosphatekinase,ATP,ADP,CTPsynthase,Ribonucleotidereductase,deoxyUDP,Nucleosidediphosphatephosphohydrolase,deoxyUMP,Thymidylatesynthase,N5,N10-MethyleneTHF,DHF,TTP,Nucleosidemonophosphatekinase,Nucleos
7、idediphosphatekinase,Myo-Inositol-3-phosphateIns(3)P1,Biosynthesis and metabolism of phosphoinositides,Glucose 6-pgosphate,Ins(3)P1synthase,Ins(3)P1phosphatase,Myo-InositolIns,PtdInssynthase,PtdIns,Myo-inositolkinase,PtdIns-4-P,PtdIns4-kinase,PtdIns5-kinase,PtdIns-4,5-P2,PI3K,PtdIns-3,4,5-P3,PLC,PLC
8、,PI3K,PtdIns-3,4-P2,Ins-1,3,4-P3,Ins-1,3,4,5-P4,Ins-1,4,5-P3,PLC,PTEN,PTEN,Ins-1,4-P3,PTEN,PTEN,Fatty acidsynthase,-ketoacylACP-synthase,Fatty acids synthesis,Acetyl CoAcarboxylase,ATP,ADP + Pi,Acetyl CoA,Malonyl CoA,Citrate,Oxaloacetate,ATP,ADP + Pi,Citrate lyase,Acetyltransacylase,Malonyltransacyl
9、ase,-ketoacylACP-reductase,-hydroxyacylACP-dehydratase,Enoyl-ACPreductase,Pantetheine,NADPH,NADP,NADPH,NADP,Cholesterol de novo synthesis IIsoprenyl formation,Acetoacetyl-CoA,Thiolase,HMG-CoAsynthase,Mevalonate,3-Hydroxy-3-methylglutaryl-CoA,Mevalonatekinase,Phosphomevalonatekinase,Mevalonate-5-pyro
10、phosphodecarboxylase,5-Phosphomevalonate,5-Pyrophosphomevalonate,3-Isopentenylpyrophosphate,3, 3-Dimethylalylpyrophosphate,Isomerase,HMG-CoAreductase,2 NADPH,2 NADP,ATP,ADP,ADP,ATP,ADP,ATP,Cholesterol,Biosynthesis of bile acids,7-Hydroxycholesterol,Sterol 7hydroxylaseCYP7A1,7-Hydroxy-4-cholesten-3-o
11、ne,3-Hydroxystroid dehydrogenaseHSD3B7,7, 12-Dihydroxy-4-cholesten-3-one,Sterol-12-hydroxylaseCYP8B1,5-Cholestan-3, 7, 12-Triol,Oxosteroid-5-reductaseAKR1D1,Sterol 27-hydroxylaseCYP27A1,3, 7, 12-Trihydroxy-5-cholestanoic acid,AKR1D1AKR1C4,5-Cholestan-3, 7-diol,12-Deoxycholic acid,3-Hydroxy-steroid d
12、ehydrogenaseAKR1C4,Biosynthesis of RNA,RNAPolymerase,Biosynthesis of DNA,DNAPolymerase,-oxidation,Mitochondrial matrix,Intermembranespace,Palmitoyl CoA,Palmitoyl CoA,Trans 2 enolopalmitoyl CoA,3-Hydroxypalmitoyl CoA,3-Ketopalmitoyl CoA,Myristoyl CoA,Acetyl-CoA,Palmitoyl CoA,Palmitoyl CoAligase,ATP,A
13、MP,Carnitineacyltransferase I,Carnitineacyltransferase II,Acyl CoAdehydrogenase,Enoyl CoAhydratase,3-Hydroxyacyl CoAdehydrogenase,Citric AcidCycle,FAD,FADH2,NAD,NADH,Cytoplasm,H2O,Myo-Inositol-3-phosphateIns(3)P1,Phospho-myo-inositol Toolkit 2,Myo-Inositol-3,4-diphosphateIns(3,4)P2,Myo-Inositol-3,6-
14、diphosphateIns(3,6)P2,Myo-Inositol-3,4,6-triphosphateIns(3,4,6)P3,Myo-Inositol-1,3,4-triphosphateIns(1,3,4,)P3,Myo-Inositol-3,4,5,6-tetraphosphateIns(3,4,5,6)P4,Myo-Inositol-1,4,5-triphosphateIns(1,4,5)P3,Myo-Inositol-1,3,4,6-tetraphosphateIns(1,3,4,6)P4,Myo-Inositol-1,3,4,5-tetraphosphateIns(1,3,4,
15、5)P4,Myo-Inositol-1,4,5,6-tetraphosphateIns(1,4,5,6)P4,Myo-Inositol-1,2,3,4,6-pentaphosphateIns(1,2,3,4,6)P5,Myo-Inositol-1,3,4,5,6-pentaphosphateIns(1,3,4,5,6)P5,Myo-Inositol-1,2,3,4,5-pentaphosphateIns(1,2,3,4,5)P5,Myo-Inositol-1,2,4,5,6-pentaphosphateIns(1,2,4,5,6)P5,Myo-Inositol-1,2,3,4,5,6-hexa
16、phosphateIns(1,2,3,4,5,6)P6 (Phytic acid),Myo-Inositol-3-phosphateIns(3)P1,Phospho-myo-inositol Toolkit,Myo-Inositol-3,4-diphosphateIns(3,4)P2,Myo-Inositol-3,6-diphosphateIns(3,6)P2,Myo-Inositol-3,4,6-triphosphateIns(3,4,6)P3,Myo-Inositol-1,3,4-triphosphateIns(1,3,4,)P3,Myo-Inositol-3,4,5,6-tetrapho
17、sphateIns(3,4,5,6)P4,Myo-Inositol-1,4,5-triphosphateIns(1,4,5)P3,Myo-Inositol-1,3,4,6-tetraphosphateIns(1,3,4,6)P4,Myo-Inositol-1,3,4,5-tetraphosphateIns(1,3,4,5)P4,Myo-Inositol-1,4,5,6-tetraphosphateIns(1,4,5,6)P4,Myo-Inositol-1,2,3,4,6-pentaphosphateIns(1,2,3,4,6)P5,Myo-Inositol-1,3,4,5,6-pentapho
18、sphateIns(1,3,4,5,6)P5,Myo-Inositol-1,2,3,4,5-pentaphosphateIns(1,2,3,4,5)P5,Myo-Inositol-1,2,4,5,6-pentaphosphateIns(1,2,4,5,6)P5,Myo-Inositol-1,2,3,4,5,6-hexaphosphateIns(1,2,3,4,5,6)P6 (Phytic acid),Glycogen,Glycogenin,8-oxoguanine,A common DNA lesions Toolkit I,O6-methylguanine,Uracil,Thymine gl
19、ycol,1, N6-ethenoadenine,3-methyladenine,Cyclobutane pyrimidine dimers,Photoadduct,Ribose,Glucuronate,Arabinose,Fructose,Glucose,Galactose,Mannose,Fucose,Sialic Acid,Inositol,D-Abequose,L-Rhamnose,A carbohydrate toolkit,PGH2,TXA2,TXB2,Bioactive lipids - Prostanoids,PGD2,PGJ2,PGE2,PGA2,PGB2,PGF2,PGF-
20、M,PGI2,6-keto-PGF1,Bioactive lipids - Leukotrienes,5-HETE,LTA4,LTB4,LTC4,LTD4,LTE4,20-OH- LTE4,LXA4,LTB4,LTF4,LXA,LXB,Bioactive lipids,Arachidonate,Ceramide,Sphingosine-1-phosphate,PAF,Phosphatidic acid,Anandamide,2-Arachidonoylglycerol,qGTP,NO mediated attenuation of adrenergic Gq signaling in VSMC
21、,qGDP,PLC,qGTP,Ca,sGC,ANP,PKG,PKC,MLCK,myosin,myosin,myosin,P,P,CaM,MLCK,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,K+,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,Ca,CaM,Ca,PA,1,4,5-Ins,P,SGTP,RGS function and regulation,SGDP,AC,PKA,cAMP,cAMP,cAMP,cAMP,cAMP,cAMP,cAMP,AC,SGTP,Ligand,Ligand,P
22、,P,cAMP,cAMP,cAMP,TNF,Modular Structure of Cytokine Receptors,IFNR2515 aa,IFNR1 530 aa,IL-6R 449 aa,IL-11R 400 aa,IL-10R1 557 aa,IL-10R2325 aa,STATbinding,IL-13R1 427 aa,IL-4R 825 aa,FNIII,FNIII,CCCC,WS-WS,FNIII,FNIII,FNIII,FNIII,FNIII,FNIII,FNIII,FNIII,FNIII,FNIII,SOCS,gp130 896 aa,STATbinding,Tyk2
23、,Jak1,INFR1 489 aa,STATbinding,FNIII,FNIII,Jak1,IL-22R1 574 aa,STATbinding,FNIII,FNIII,Jak1,Jak1,INFR2 332 aa,FNIII,FNIII,Jak2,STATbinding,Tyk2,Jak1,Jak2,Jak1,IL-13R2 380 aa,FNIII,FNIII,CCCC,WS-WS,FNIII,FNIII,CCCC,WS-WS,FNIII,FNIII,FNIII,STATbinding,STATbinding,Ig,Ig,Ig,Ig,OSM-R952 aa,Jak3,FNIII,FNI
24、II,FNIII,FNIII,STATbinding,Ig,FNIII,FNIII,CCCC,WS-WS,FNIII,FNIII,CCCC,WS-WS,FNIII,FNIII,CCCC,WS-WS,LIF-R 1053 aa,Jak3,FNIII,FNIII,FNIII,STATbinding,Ig,FNIII,WS-WS,FNIII,FNIII,CCCC,WS-WS,STATbinding,STATbinding,SHP,FNIII,CCCC,STATbinding,STATbinding,Tyk2,FNIII,FNIII,CCCC,WS-WS,Ig,SOCS,SHP,FNIII,FNIII
25、,CCCC,WS-WS,Ig,Jak3,c 349 aa,IRS,FNIII,FNIII,CCCC,WS-WS,Ig,Jak3,FNIII,FNIII,FNIII,FNIII,FNIII,CCCC,WS-WS,IL-12 662 aa,FNIII,WS-WS,SOCS,IRS,STATbinding,Ig,Tyk2,Fibronectintype III domain,Ig - like,IRS-binding,Fibronectintype III domainwith cysteines,SOCS-binding,STAT-binding,JAK/TYK-binding,Fibronect
26、intype III domainwith WSXWS,SHP-binding,SHP,Adaptor proteins - selected (human),SAMdomain,Src-homology 3 domain,SH3,SH2,Src-homology 2 domain,GRB2,PTBdomain,PTB,Cysteine-richregion 1,Calponinhomology domain,C-1,Calponin,Sprouty,Sprouty domain,SLAP,Crk,GADS (GRID),Nck1,BLNK,Rho-GEF,Rho-GEF homologydo
27、main,Vav-1,SLP-76,SHC-1,APS,SAM,PH,Plekstrin homologydomain,Protein separation using SDS-PAGE(Laemmli system),Stackinggel,Resolvinggel,1. Apply protein/dye samples into polyacrylamide gel wells,2. Run the electrophoresis until dye reaches the end of the gel,3. Remove the gel from the apparatus and s
28、tain for proteins,A Column Chromatography Toolkit,Embryonic stem cells,Egg donor,Sperm donor,Egg fertilization,Culture fertilized cells forseveral cell divisions,Dissociate cell clusterfor subculturing andfurther utilization,Freeze in N2 forlong-term storage,Use for In Vitrofertilization,Potential u
29、se fortherapeutic cloning,Common Cloning Vectors,Metabolism of capecitabine,5-FU,5-Fluorouridine,5-FUMP,5-FdUMP,Uridine phosphorylase,5-Fluorodeoxyuridine,Thymidine phosphorylase,Orotatephosphoribosyltransferase,Thymidine kinase,5-FUTP,5-FdUTP,RNADNA,5-nucleotidase,5-nucleotidase,Uridine kinase,ATP,
30、ADP,ATP,ADP,PRPP,CapecitabineXeloda(prodrug),Carboxyl-esterase,5-Fluoro-5-deoxycytidine,Cytidinedeaminase,Thymidine phosphorylase,5-Fluoro-5-deoxyuridine,Pharmacologicallyactive compound,Pertussistoxin,NAD,ADP,Choleratoxin,AC,ATP,cAMP,AC,SGTP,Ligand,Pharmacological modulation of cAMP,Ligand,Ligand,Ligand,NAD,ADP,Forskolin,Rolipram,AMP,PDE,Mechanism ofPenicillin(inhibition of crosslinkingpeptidoglycan),
