1、USP 美国药典 液相方法色谱柱汇总默认分类 2010-02-22 16:50:57 阅读 297 评论 0 字号：大中小 订阅 液色迷人 http:/USP 即美国药典，为高效液相色谱柱填料规定了一些指标，即 USP L1-USP L60 系列。用户经常会在文献和技术开发中遇到用符合 USP L 系列方法的液相柱分离的要求，为此我们在此把我们公司能够提供的符合此方法的液相柱作一列表，供您参考！USP 填料描述 推荐色谱柱L1 C18 烷基化学键合于多孔硅胶或陶瓷微粒，3-10u C18: COSMOSIL, Polaris,L2 C18 烷基化学键合于多孔硅胶，30-50u C18 分离填料
2、： FUJI 填料 L3 多孔硅胶微粒，5-10u SIL: COSMOSIL, PolarisL4 硅胶微粒，30-50u L5 氧化铝微粒，30-50u L6 强阳离子交换填料：磺化氟碳聚合物涂于固体球形核粒上，30-50u L7 C8 烷基化学键合于 3-10u 完全多孔硅胶微粒 C8: COSMOSIL, Polaris,L8 单分子层丙氨基化学键合于 10u 硅胶载体， NH2: COSMOSIL, Polaris,L9 强酸性阳离子交换基团键合于 10u 无定形多孔硅胶， SCX:Nucleosil SA; HYPERSILL10 氰基化学键合于 5-10u 多孔硅胶颗粒， CN:
3、COSMOSIL, PolarisL11 苯基化学键合于 5-10u 多孔硅胶颗粒， 苯基柱:COSMOSIL,KROMASILL12 季铵基化学键合于 30-50u 固体硅胶小球上，强阴离子交换填料 L13 三甲基硅烷化学键合于 5-10u 多孔硅胶颗粒， C1(TMS):COSMOSIL, KROMASILL14 带强碱性季铵基的阴离子交换涂料化学键合于 10u 硅胶微粒， SAX: Nucleosil SB;Hypersil;ZorbaxL15 六烷基化学键合于全多孔硅胶颗粒，3-10u C6:Allsphere,ChromegabondL16 二甲基化学键合于全多孔硅胶颗粒，5-10u
4、 C2:Nucleosil,AdsorbosilL17 强阳离子交换树脂：氢型、磺化交联的苯乙烯二乙烯基苯共聚物，7-11u Hamilton HC 75 氢型柱, PL- Hi-Plex HL18 氨基和氰基官能团键合于 5-10u 的多孔硅胶颗粒上 Partsil PACL19 强阳离子交换树脂：钙型、磺化交联的苯乙烯二乙烯基苯共聚物，9u Hamilton HC 75 钙型柱，PL- Hi-Plex CaL20 二羟基丙烷基团化学键合于多孔硅胶颗粒，5-10u Diol:NucleosilL21 刚性的苯乙烯二乙烯基苯共聚物小球，5-10u PRP-1:Hamilton；PLRP-SL2
5、2 阳离子交换树脂：带磺酸基团的多孔苯乙烯聚合物小球，外径约 10u Hamilton PRP-X200, L23 阴离子交换树脂：带季铵基团的聚甲基丙烯酸甲酯或聚丙烯酸甲酯多孔胶体，约 10u 外径TSK-DEAE-5PW, COSMOGEL QAL24 基体表面带大量羟基团的聚乙烯构成的半刚性亲水凝胶，32-63u 外径 L25 该填料应能分离分子量从 100-5000 道尔顿的化合物，用于分离中性，阳离子型及阴离子型的水溶性聚合物。以聚甲基丙烯酸甲酯树脂为载体，交联聚甲氧基乙醚，表面含有残留羰基基团GFC:Macrosphere(7u),Shodex Ohpak SB-802.5L26
6、丁基硅烷化学键合于全多孔硅胶颗粒上，5-10u C4:COSMOSIL, KROMASILL27 多孔硅胶颗粒，30-50u 外径 L28 100 埃的高纯、球形硅胶键合以阴离子性以及 C8 基团的多功能载体 液色迷人http:/L29 氧化铝，反相，含碳率低，氧化铝基的聚丁二烯小球，5u，80 埃 GammaBond RP1L30 乙基硅烷化学键合于多孔硅胶上，3-10u 外径 C2:Nucleosil,AdsorbosilL31 强阴离子交换树脂：季铵基键合于乳胶颗粒上，分散于 8.5u 大小，孔径2000 埃的乙基乙烯基苯和二乙烯基苯（55%）的交连聚合物颗粒中液色迷人http:/L32
7、 配位体交换手性填料：L脯氨酸铜共价键合于不规则硅胶颗粒上，5-10u COSMOSIL CSP-HPR,Nucleosil chiral-1L33 能够分离 4000-400，000 道尔顿的蛋白质，球形硅胶载体，具 PH 稳定性Nucleosil 125-5 GFC,TSK G3000SWL34 强阳离子交换树脂：铅型、磺化交联的苯乙烯二乙烯基苯共聚物，9u Hamilton HC 75 铅型柱，Hi-Plex Pb L35 经锆强化的球形硅胶颗粒，亲水性单分子层键合，孔径 150 埃 Zorbax GF-250L36 L苯基甘氨酸的 3,5二硝基苯甲酰衍生物共价键合在 5um 的氨丙基硅
8、胶上Nucleosil chiral-3 手性柱L37 能分离 2，000 40，000 分子量蛋白质的聚甲基丙烯酸凝胶 Shodex OHpak SB-803HQ L38 分离水溶性样品的甲基丙烯酸机质的体积排阻填料 Shodex OHpak SB-802HQL39 亲水性 聚羟甲基丙烯酸 全多孔球形树脂 Shodex OHpak SB-800 系列L40 3，5 二甲苯甲氨酸纤维素涂布在 5 到 20um 的多孔硅胶上 CHIRALCEL OD; NUCLEOCEL DELTAL41 固化 ALPHA 酸糖蛋白在 5um 的硅胶颗粒上 Chiral-AGP，L42 C8 和 C18 烷基化
9、学键合于多孔硅胶微粒，5u L43 五氟苯基化学键合在 5-10u 的硅胶微粒上 TAXSIL 五氟苯基柱：常做紫杉醇分析用L44 A multifunctional support,which consists of a high purity, 60?, spherical silica substrate that has been bonded with a cationic exchanger,sulphonic acid functionality in addition to a conventional reversed-phase C8 functionality.Chrom
10、egabond RP-SCXL45 Beta cyclodextrin bonded to porous silica particles, 5 to 10um in diameterNUCLEODEX -OH; ChirDexL47 High capacity anion exchagne microporous substrate,8um in diameter,dimension 250 x 4.0mmDionex CarboPac MA1L49 A reverse-phase packing made by coating a thin layer of polybutadiene o
11、n to a spherical porous zirconia particles, 3 to 10umPBD 柱L50 A strong cation exchange resin made of porous silica with sulphopropyl groups, 5 to 10um in diameter.TSK IC SWL51 Amylose tris-3,5-dimethylphenylcarbamate-coated,porous,spherical,silica particles,5 to 10um in diameterChiralpak AD; NUCLEOC
12、EL ALPHAL52 A strong cation-exchange resin made of porous silica with sulphopropyl groups,5 to 10um in diameter.TSKgel IC-Cation , TSKgel SP-2SWL53 Weak cation-exchange resin consisting of ethylvinylbenzene,55% cross-linked with divinylbenzene polymer,3 to 15um in diameter.Substrate is surface graft
13、ed with carboxylic acid and/or phosphoric acid functionalized monomers.Capacity not less than 500uEq/column.IonPac CS14L54 A size exclusion medium made of covalent bonding of dextran to highly cross-linked porous agarose beads, about 13um in diameter.Superdex Peptide HR 10/30L55 A strong cation-exch
14、ange resin made of poroous silica coated with polybutadiene-maleic acid copolymer,about 5um in diameter.L56 Propyl silane chemically bonded to totally porous silica particles,3 to 10um in diameterZorbax SB-C3L57 Chiral recognition protein, ucoid, chemically bonded to silica , about 5um, with a pore
15、size of 120AES-OVM 手性柱L58 Strong cation-exchange resin consisting of sulphonated cross-linked PL Hi-Plex Nastyrene-divinylbenzene copolymer in the sodium form, about 7 to 11um diameterL59 Packing having the capacity to separate proteins by molecular weight over the range of 10 to 500kDa. It is spher
16、ical(10um), silica-based,and processed to provide hydrophilic characteristics and pH stabilityTSKgel G3000SWL60 Spherical porous silica gel,3 or 5um in diameter, the surface of which has been covalently modified with palmitamidopropyl groups and endcapped.NUCLEOSIL C18 NautilusL61 A hydroxide-select
17、ive,strong anion-exchange resin consisting of a highly cross-linked core of 13um microporous particles having a pore size less than 10 angstrom and consisting of ethylvinylbenzene cross-linked with 55% divinylbenzene with a latex coating composed of 85nm diameter microbeads bonded with alkanol quaternary ammonium ions(6%)IonPac AS-11L62 C30 silane bonded phase on a fully proous spherical silica,3 to 15um in diameterDevelosil C 30, YMC C30
