1、Welcome!,X-Ray Facility, 410-413 Kasha Laboratory Institute of Molecular Biophysics Florida State University Tallahassee, FL 32306-4380 www.sb.fsu.edu/xray, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,2,X-Ray Facility Orientation,History of the Facility Facility Resources Requirements Data Collec
2、tion Data Processing & Manuals Archiving & Summary Contact & Information, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,3,History of the Facility-1,Core facility planned in 1990-92 Facility Director hired Feb 1993 Construction June Sep 1993 Two generators & Image Plate (IP) detector in January 1994
3、 Elliott generator in 1995,Important Dates, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,4,History of the Facility-2,Cryo capabilities in 1996 Crystal chamber in 1999 Confocal mirror in Spring 2000 CCD w/ confocal mirror in Fall 2000 CryoCooler 700 w/ auto-refill in Fall 2000 Additional Linux mach
4、ines in 2000 Complete renovation during 2002-03,Important Dates, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,5,Facility Resources & Accessories,Three Copper rotating anode generators Two automated area detectors Two confocal & one total reflection mirrors Two cryo coolers One automated liquid Nit
5、rogen refiller Linux and Windows NT computers Full time Faculty/Staff (State-line), Thayumanasamy Somasundaram, 2003-2004,9/4/2018,6,Resources-1,GeneratorsTwo Rigaku H2R 5.4 kW generatorsOne Elliott GX-30 3.0 kW generator Area detector-1 (IP)R-Axis IIc (1994) w/ confocal blue mirror65-450 mm sample-
6、detector distance0-25 degree non-zero 2-theta capabilityOxford Cryostream 600 cold head, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,7,Resources-2,Area detector-2 (CCD)Mar165 CCD w/ confocal purple mirror35-380 mm sample-CCD distanceLimited non-zero 2-theta capabilityOxford Cryostream 700 cold he
7、ad Long & short Supper (total-reflection) mirrors Stand-alone IP Bio Unit I Analytical Lab, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,8,Accessories-1,Leica stereo microscope w/ Moticam digital camera Nikon Optiphot microscope w/ analog camera Stereo zoom microscopes DVD-R & +R writers DDS-3 & -
8、4 tape drives FireWire (Maxtor: 60 & 80 GB) drives Windows NT and five Linux computers, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,9,Accessories-2,Cold room for 4C crystallization set-up Room for ambient crystallization set-up Crystallization chamber Temperature & humidity monitoring Dry Cryo Sh
9、ipper Long-term sample storage Laptop for synchrotron trips, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,10,Requirements,Member of Structural Biology Program Radiation safety training Computer account (UNIX/LINUX/NT) Body and/or finger radiation badgeNo cost to use Users bring consumables:CryoLoo
10、ps, Capillaries, Tape and DVD media, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,11,Data Collection-1,Sample mounting decisionCapillary or loop mounting? Data collection temperatureAmbient or cryo? If cryo, cryo protection selectionGrow or soak crystal? Detector decisionImage plate or CCD detecto
11、r?, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,12,Data Collection-2,Screening or regular data collection? Improvement or brand-new crystal? Resolution decisionLow, moderate, or high-resolution? 2-theta angle Cell dimension/detector distance?, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,13,Dat
12、a Collection Procedure,Check the schedule at:www.sb.fsu.edu/soma/XraySchedules/schfrmset.html Request timeby e-mail: somasb.fsu.eduby phone: 644-6448 Priority given for samples in the beam Priority given to unstable samples Flexible schedule & co-operation is valued, Thayumanasamy Somasundaram, 2003
13、-2004,9/4/2018,14,Data Processing Procedure,Collect preliminary data & auto index If successful, complete data collectionIf not, modify procedure and try Index, integrate, scale full data set Repeat integration & scaling, if needed Validate and compare Archive & transfer files, Thayumanasamy Somasun
14、daram, 2003-2004,9/4/2018,15,Archiving,Data is the responsibility of the “USER” Available archiving resources:DDS-3 & DDS-4 tape drivesDVD-R & +R writers Users bring the media Facility archives data (last resort) Computer Resources may be helpful, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,16,Ma
15、nuals & Documents,Online version athttp:/www.sb.fsu.edu/xray/Manuals/soft_man.html Paper version in 410 KLB (XRF) Program authors website Manufacturers website IMB resource pages Lab protocols and procedures, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,17,Summary,State of the art facility Excelle
16、nt scientific/support staff:Computer Resources, Protein Expression Facility, Physical Biochem Facility, Electronics shop, & Machine shop Keep it clean and in working order Report problems to Soma (4-6448), Thayumanasamy Somasundaram, 2003-2004,9/4/2018,18,Contacts & Information-1,Data collection, pr
17、ocessing, scheduling: Thayumanasamy Somasundaram SOMA Ph: 644-6448 | E-mail: somasb.fsu.edu www.sb.fsu.edu/soma | www.sb.fsu.edu/xray XRF (KLB 410-413) | 645-1333 Computer related: Mike Zawrotny Ph: 644-0069 | zawrotnysb.fsu.edu, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,19,Contacts & Informati
18、on-2,Electronics: Rick Wang Ph: 645-3309 | wangsb.fsu.edu Machine Shop | Wood shop: Wade Baggett or Ed Kirkland Ph: 644-1419 | 645-1338, Thayumanasamy Somasundaram, 2003-2004,9/4/2018,20,Picture Gallery,IP & CCD Detectors IMB (2001), Thayumanasamy Somasundaram, 2003-2004,9/4/2018,21,Picture Gallery,IP & CCD Detectors DLC (2002), Thayumanasamy Somasundaram, 2003-2004,9/4/2018,22,Picture Gallery,IP & CCD Detectors KLB (2004), Thayumanasamy Somasundaram, 2003-2004,9/4/2018,23,Picture Gallery,Crystal Room & Computer Room (2004), Thayumanasamy Somasundaram, 2003-2004,
