1、5/430061-6 IL-6-8 IL-8-TNF-40 SPF Wistar CAS Elisa IL-6 IL-8 TNF-3 P0.05 P0.05 P0.05 3 0.05 P0.05 3 IL-6 IL-8 TNF-P0.05 IL-6 IL-8 TNF-P0.05 P0.05 IL-6 IL-8 TNF-6-8-R285.5 A doi 10.3969/j.issn.1008 987x.2023.02.01Effect of acupuncture and moxibustion with Biao-Ben acupoint combination on ti-ght junct
2、ion and inflammatory factor in serum in IBS-D model ratsLI Yi-hong HU Jiang-shan LI Jia WANG Hua Hubei University of Chinese Medicine/Hubei Collaborative Innovation Center of Acupuncture and Moxibustion forDisease Prevention Wuhan 430061 Abstracts Objective To observe the general signs gastric empty
3、ing rate small intestine propulsion rate intestinal his-tomorphology and serum content of diarrhea-predominant irritable bowel syndrome model rats with acupuncture and acupunctureEffects of interluekin-6 IL-6 IL-8 and tumor necrosis factor-TNF-expression.Methods Forty SPF female Wistar ratswere rand
4、omly divided into blank group model group Biao-Ben acupoint combination group and regular acupoint combinationgroup.The irritable bowel syndrome rat model was established by combining acute and chronic stress with CAS method.UseSimultaneous determination to detect fecal water content gastric emptyin
5、g rate small intestinal propulsion rate transmissionelectron microscope to observe rat intestinal mucosal ultrastructure and Elisa method to detect the contents of IL-6 IL-8 and 81973931 2018 284 1998-2020 1955-E-mail 6 TNF-in rat plasma.Results Compared with blank group the fecal water content of t
6、he other three groups increased P0.05 the fecal water content of Biao-Ben acupoint combination group and regular acupoint combination group was lower thanthat of model group P0.05 and Biao-Ben acupoint combination group was lower than model group in regular acupointcombinations group P0.05 compared
7、with blank group the gastric emptying rate of the other three groups was increased and the small intestine propulsion rate was decreased P0.05 compared with blank group the intestinal mucosal structure ofthe rats in model group was damaged and the ultrastructure of the intestinal mucosa of the rats
8、in Biao-Ben acupoint combinationgroup and regular acupoint combination group after treatment was relatively more intact compared with model group and Biao-Ben acupoint combination group was better than regular acupoint combinations group compared with blank group the serum levels of IL-6 IL-8 and TN
9、F-in the other three groups were all increased P0.05 compared with model group the levels of IL-6 IL-8 and TNF-of Biao-Ben acupoint combination group and regular acupoint combination group weredecreased P0.05 and the Biao-Ben acupoint combination group was lower than regular acupoint combination gro
10、up P0.05.Conclusion Acupuncture with acupoints of Biao-Ben acupoint combination can improve the general behavioralsigns gastric emptying rate small intestinal propulsion rate and intestinal histomorphology in IBS-D rats and reduce theexpressions of serum inflammatory factors IL-6 IL-8 and TNF-thereb
11、y reducing the inflammatory factors and relievingsymptoms in patients with diarrhea-predominant irritable bowel syndrome.Keywords Acupuncture Biao-Ben acupoint combination Irritable bowel syndrome IL-6 IL-8 TNF-Irritablebowelsyndrome IBS 1 IBS 4 diarrhea-predominantIBS IBS-D constipation-predomi-nan
12、tIBS IBS-C mixed-type IBS IBS-M unsubtyped IBS IBS-U IBS-D 2-4 IBS-D 5-6 7 8 IBS 9 IBS-6 IL-6-8 IL-8-TNF-IBS-D IBS-D 1 1.1 3 SPF Wistar 40 22020 g SCXK 2020-0018 2225 12 h/12h 1 1.2 IL-6 ELISA IL-8ELISA TNF-ELISA 324017-002 324317-002 291276-001 ST-360 aquaplus2 TGL-10B 1.3 7d 40 4 10 CAS IBS-D 10 7
13、 24h 24 h 1 min 45min 45 5 min 4 3 min 12 h 1d 3 1 28 1 h 6h/100%11 21d IBS-D 21d IBS-D 12 Yin 13 7 0.30 mm13 mm 0.5 cm 23cm 15min 1/d 21d IBS-D 0.30 mm 13mm 23cm 15min 1/d 21d 1.4 1.4.1 4 24 h M M=M-M/M 100%1.4.2 24 h W 1 1 g/mL 0.8 mL/30min W 2 W 3 L 1 L 2=1-W 2-W 3/W 1 100%=L 2/L 1 100%1.4.3 4%2%
14、1.4.4 IL-6 IL-8 TNF-4 12000g 10 min ELISA IL-6 IL-8 TNF-1.5 SPSS 22.0 0.05 3 0.05 0.05 0.05 0.05 1 1%n=10 40.252.97 40.543.53 40.333.64 54.343.00*#39.962.43 44.182.47*#40.752.35 46.422.31*#*0.05#0.05 0.05 0.05 2.2 3 0.05,0.05 0.05 0.05 0.05 0.05 2 2%n=10 61.473.50 50.674.99 50.233.70 79.132.56*56.77
15、3.93*#58.515.82*#52.262.81*#75.043.34*#*0.05#0.05 0.05 2.3 8 1 1 5000 2.4 IL-6 IL-8 TNF-IL-6 IL-8 TNF-0.05 IL-6 IL-8 TNF-0.05 IL-6 IL-8 TNF-0.05 3 3 IL-6 IL-8 TNF-ng/mL n=10 IL-6 IL-8 TNF-47.622.50 51.393.83 179.264.27 89.351.92*77.012.48*189.583.57*56.744.27*#65.444.20*#183.542.56*#68.485.44*#70.97
16、2.86*#185.603.22*#*0.05#0.05 0.05 3 IBS IBS IBS 14 15-16 IBS 17-18 TNF-IL-6 IL-8 IBS-D TNF-19 TNF-IL-6 IL-6 20 IL-8 TNF-21-22 IL-8 IL-1 TNF-IBS 23 IBS 24 IBS 25-26 27 28 IBS IBS-D IL-6 IL-8 TNF-IBS-D IBS 9 1 Grayson M.Irritable bowel syndrome J.Nature 2016 533 7603:S101.2 Drossman DA Hasler WL.Rome-
17、functional GI disorders:disorders of gut-brain interaction J.Gastroenterology 2016 150 6:1257-1261.3.2017 J.2018 26 3:227-232.4.2018 J.2019 31 8:944-946.5.30 J.2018 59 23:2034-2036.6.J.2014 29 2:517-519.7 Han Y Ma TM Lu ML et al.Role of moxibustion in in-flammatory responses during treatment of rat
18、ulcerative coli-tis J.World J Gastroenterol 2014 20 32:11297-11304.8.J.2016 36 7:737-739.9.J.2016 28 2:32-37.10 Li S Fei G Fang X et al.Changes in Enteric Neurons ofSmall Intestine in a Rat Model of Irritable Bowel Syndromewith Diarrhea J.J Neurogastroenterol Motil 2016 22 2:310-320.11.5-J.2020 45 7
19、:535-540.12.M.:2007:135.13 Yin CS Jeong HS Park HJ et al.A proposed transposi-tional acupoint system in a mouse and rat model.Res VetSci 2008 84 2:159-165.14.J.2019 22 11:1214-1219.15 Choghakhori R Abbasnezhad A Hasanvand A et al.In-flammatory cytokines and oxidative stress biomarkers in ir-ritable
20、bowel syndrome:Association with digestive symp-toms and quality of life J/OL.Cytokine 2017 93:34-43.16 Ohman L Simrn M.Pathogenesis of IBS:role of inflam-mation immunity and neuroimmune interactions J.NatRev Gastroenterol Hepatol 2010 7 3:163-173.17.J.2011 38 3:594-596 18 Li Y Jin K Van P et al.c-My
21、b Enhances Breast CancerInvasion and Metastasis through the Wnt/-Catenin/Axin2Pathway J.Cancer Res 2016 76 11:3364-3375.19 Papadakis KA Targan SR.Tumor necrosis factor:biologyand therapeutic inhibitors J.Gastroenterology 2000 119 4:1148-1157 20.J.2001 13 2:111-113 21 Matricon J Meleine M Gelot A et
22、al.Review article:as-sociations between immune activation intestinal permeabi-lity and the irritable bowel syndrome J.Aliment Pharma-col Ther 2012 36 11-12:1009-1031 22.5-HT IL-8 J.2015 15 5:89-91.23.J.2016 24 10:794-796.24.2017 J.2017 58 18:1614-1620.25.J.2021 36 9:5162-5164.26.J.2004 4:3-5.27.J.2021 32 3:729-731.28.D2 J.2018 43 1:24-28.2022-09-05
