1、蛋白质印迹(Western Blot)Contents 目录 01 02 03 Blot(Western Blot)https:/1975 Edwin Mellor Southern DNA Southern 1977 George Stark RNA 1979 1979 George Stark 1981 W.Neal Burnette Western George Stark Edwin Mellor Southern W.Neal Burnette 蛋白质印迹原理 Millipore 2015 蛋白质印迹原理示意图 PAGE NC:Y:Y Y Y Y Y Y Y Y Y Y Y Y Y
2、1 2 PAGE IC 蛋 白 质 印 迹的应 用 01 蛋 白 质 印 迹的应 用 03-pull down co-IP 蛋 白 质 印 迹的应 用 04 蛋 白 质 印 迹的应 用 02 1.Thermofisher 2.Millipore 2015 3.Southern,E.M.(1975).Detection of specific sequences among DNA fragments separated by gel electrophoresis.Journal of Molecular Biology.98(3):503 517.4.Renart,J.;Reiser,J.;
3、and Stark,G.Transfer of proteins from gels to diazobenzyloxymethyl-paper and detection with antisera:a method for studying antibody specificity and antigen structure.Proc.Natl.Acad.Sci.USA.1979;76:3116 3120.5.dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A.Anal.Biochem.1981;112:195 203.敬请关注 下一讲：蛋白质印迹实验流程
