1、Symmetry色谱柱的维护和使用指南 第一节 启用 a g14406g16901g7621g11352g4445g16025 1 g1926g8939g2559g7389g1231g1321g13543g1926g11428g11352g8905g13007g13491g712g4570g14406g16901g7621g1849g2487g12483g17842g6521g2052g17839g7691g3132g11352g1998g2487g12483g712g14406g16901g7621g990g11352g12673 g3848g7643g7138g1114g8981g2172
2、g11468g11352g8981g2533g708g1820g993g16213g6521g7828g8991g3132g709g452 2 g3324 0 1ml min g8981g17907g7477g1226g991g11004 100 g11014g18267g6122g1069g14108g1926g8939g14406g16901g7621g712g9994g2530g3324 5min g1881g4570g8981g17907g2331g14279 1 0ml ming452 3 g5415g9354g9094g3355g2260g3332g1186g7621g4388g1
3、998g2487g8981g1998g7114g712g1584g6493g8981g17907g712g4570g14406g16901g7621g1998g2487g12483g6521g2052g7828g8991g3132g990g708g17837g7691g6817g1328 g2499g1209g18003g1825g8680g8885g17839g1849g7828g8991g13007g13491g712g5194g1000g2499g5567g17907g17810g2052g3534g13459g5191g15925g452g709 4 g6365g10043g8505g
4、206002g11352g7053g8873g17892g9188g6564g20652g8981g17907g452 5 g980g7098g2399g2159g17810g2052g12295g4462g1552g5194g1000g3534g13459g5191g15925g3921g2530g712g2029g17839g1849g991g980g8505g6817g1328g452 b g14406g16901g7621g5191g15925 Symmetryg14406g16901g7621g7171g11004100 g1069g14108g1457g4396g11352g712
5、g3324g1363g11004g1866g4439g11352g8981g2172g11468g13007g13491g2081g980g4462g16213g11842g1457g8981g2172g11468g2656g14406 g16901g7621g1881g9354g2070g11352g256g11468g4493g5627g257g452g14279g4581g1100410g1505g7621g1319g12227g11352g8981g2172g11468g5191g15925g14406g16901g7621g708g16277g159321g1025g993g2528
6、g16280g7696g14406g16901 g7621g11352g7621g1319g12227g6980g1552g709g452g1038g1114g18003g1825g8981g2172g11468g1025g11352g13543g1926g11428g3324g14406g16901g7621g6122g13007g13491g1025g7524g1998g712g3324g6454g990g8981g2172g11468g1055g2081g712 g19668g110045g1505g7621g1319g12227g11352g8712g994g7389g7438g935
7、4g2070g11352g9163g2524g9354g9094g708g4570g13543g1926g9354g9094g11004g8712g7379g1207g712g16213g8726g7389g7438g9354g2070g11352g2559g18339g8616 g8981g2172g11468g1025g11352g2559g18339g11065g1314g6122g11468g12573g712g3926g3324g1363g1100460 40g11014g18267 g13543g1926g11428g8981g2172g11468g1055g2081g1820g1
8、10045g1505g7621g1319g12227g1135260 40 g11014g18267 g8712g9354g9094g1926g8939g14406g16901g7621g709g452 g159321g726g993g2528g16280g7696g14406g16901g7621g11352g7621g1319g12227g708mLg709g708g1068g120910g1328g1038g1926g8939g14406g16901g7621g6164g11004g11352g8981g2172g11468g1319g12227g709 g6564g12046g726g
9、3926g7536g8981g2172g11468g1025g2559g7389g1314g8999g5242g11352g8981g2172g11468g6925g5627g2070g3926g12175g4388g4557g16809g2070g712g16213g17810g2052g4448g1852g5191g15925g2499g14033g19668g16213 100g1505g10990g14279200g1505g7621g1319g12227g11352g8981g2172g11468g727g8504g3818g712g4557g1122g2559g7389g11014
10、g18252g6122g11014g18252g19145g11352g8981g2172g11468g1075g19668g16213g17751g19283g11352g7114 g19400g17810g2052g2033g3999g5191g15925g452 第二节 色谱柱的使用 g1038g11842g1457Symmetryg14406g16901g7621g1867g7389g7380g1351g11352g5627g14033g712g16843g17993g4444g1209g991g6817g1328g6363g4560g726 a g1457g6264g1315 g33
11、24g17839g7691g3132g2656g2010g7524g7621g1055g19400g1363g11004g3647g7021g2282g4410g2656g20075g12902g3835g4579g18129g17878g2524g11352g1457g6264g1315g4557g1122g1457g6264g2010g7524g7621g14279g1863g18337g16213g452 g1457g6264g7621g19668g16213g4462g7411g7368g6454g712g7368g6454g2620g7411g1039g16213g2474g1927
12、g1122g7691g2709g11352g5190g1940g12255g5242g452 b g7691g2709g2081g3800g10714 g11004g8981g2172g11468g6122g8616g8981g2172g11468g8939g14085g5390g5242g5381g11352g9354g9094g708g2559g7389g17751g4581g7389g7438g9354g2070g709g9354g16311g6122g12244g18334g7691g2709g2499g1209g14731g5483g7380g3921 g11352g4804g543
13、0g2656g7828g8991g9801g6947g5242g452g3926g7536g7691g2709g3324g8981g2172g11468g1025g993g9354g16311g6122g13785g9354g16311g5242g5468g5058g7114g712g16213g11842g1457g7691g2709g451g7691g2709g9354 g9094g2656g8981g2172g11468g7171g1126g9354g11352g712g1209g18003g1825g7691g2709g3324g8981g2172g11468g1025g7524g19
14、98g452g110040 2umg11352g9400g14192g17819g9400g7691g2709g712g17885g6333g9400g14192g7114 g16213g8892g5859g14192g11352g7460g17148g993g1262g3324g7691g2709g9354g2070g1025g9354g16311g452 c pHg1363g11004g14551g3272 Symmetryg14406g16901g7621g11352g5326g16770pHg1363g11004g14551g3272g71712 8g452g159322g2027g1
15、998g1114g5132g11004g11352g13543g1926g9354g9094g2656g6925g5627g2070g452g1552g5483g8892 g5859g11352g7171g726g14406g16901g7621g11352g4563g2641g2656g6817g1328g9213g5242g451g13543g1926g11428g11352g8999g5242g1075g7171g4506g2011g11468g1863g11352g452 g6564g12046g726g3324g7509g12483pHg451g9213g5242g2656g2399
16、g2159g7477g1226g991g1363g11004g1262g13565g11713g14406g16901g7621g4563g2641g452 g159322g726Symmetryg14406g16901g7621g5326g16770g1363g11004g11352g13543g1926g9354g9094g1319g13007 e g2399g2159 Symmetryg14406g16901g7621g14033g3827g13796g24756000psig708400barg612240Mpag709g11352g2399g2159g712g1038g1114g14
17、731g5483g7368g19283g11352g1363g11004g4563g2641g712 g6117g1216g17836g7171g5326g16770g14406g16901g7621g4625g2499g14033g3324g1314g11224000 5000psig2399g2159g991g1363g11004g452 f Symmetryg14406g16901g7621g11352g5326g16770g1363g11004g9213g5242g717120 45g6680g8675g5242g712g20652g9213g2499g1209g6564g20652g
18、2010g12175g17885g6333g5627g451g19489g1314g8981g2172 g11468g12908g5242g5194g2528g7114g6564g20652g1268g17148g17907g10587g452g9994g13792g712g17241g17819g4472g9213g1363g11004g14406g16901g7621g4557g7621g4563g2641g18129g7389g17139g19766g5445g2721g712g1866g5445g2721g12255 g5242g2474g1927g1122g8981g2172g114
19、68pHg2656g6164g1363g11004g11352g13543g1926g1319g13007g452 第五节 色谱柱清洗 再生和保存 a g9177g8939g2656g1889g10995 g14406g16901g7621g4804g5430g2469g10995g6925g2476g451g14406g16901g4804g2010g2461g712g1998g10628g13949g4804g451g1457g11053g7114g19400g8886g2172g451g2010g12175g5242g19489g1314g6122g13785g2399g2159g233
20、1g20652g18129 g15932g7138g14406g16901g7621g15999g8757g7591g1114g712g11004g7389g7438g9354g2070g1926g8939g708g16843g8892g5859g18003g1825g11428g11352g7524g1998g709g17902g5132g2499g1209g8939g2447g17837g1135g8757g7591g10301g712 g3926g7536g7389g7438g9354g2070g9177g8939g993g14033g16311g1927g19394g20076g712
21、g16843g18331g11004g159323g7053g8873g17839g15904g14406g16901g7621g11352g9177g8939g2656g1889g10995g452 g7693g6466g7691g2709g5627g17148g1209g2462g1332g1114g16311g11352g8757g7591g10301g5627g17148g17885g6333g9177g8939g7053g8873g712g1100420g1505g11352g7621g1319g12227g11352HPLCg13435g11352g9354g2070 g9177g
22、8939g14406g16901g7621g712g4570g8981g2172g11468g9213g5242g2331g1427935 55g6680g8675g5242g2499g1209g6564g20652g9177g8939g11352g6940g10587g452g3926g7536g5756g11352g7621g4388g3324g9177g8939g2530 g7621g6940g17836g7171g8821g7389g6925g2476g712g16843g994g8791g10317g1002g1856g2508g13864g13007g452 g159323g726
23、g14406g16901g7621g9177g8939g2656g1889g10995g3800g10714g12255g5219 b g14406g16901g7621g11352g1457g4396 g3324g4472g9213g7477g1226g991g712g3926g7536g3247g3837g1055g1881g993g7171g11004 Symmetryg14406g16901g7621g712g16843g4570g7621g4388g1457g4396g3324 100 g1069g14108g1025g712g3926 g7536g14406g16901g7621g
24、3324g20652g9213g6122g7509g12483pH g7477g1226g991g1363g11004g712g3324g13479g7475g5049g1328g2530g5224g12447g2375g4570g14406g16901g7621g1457g4396g3324100 g1069g14108g1025 g1209g5322g19283g14406g16901g7621g11352g1363g11004g4563g2641g452g993g16213g4570g14406g16901g7621g1457g4396g3324g20652g8616g1375g1135
25、2g8712g11468g8981g2172g11468g1025g708g3926g7389g7438g9354g2070g2559g18339g1314 g112220 g712g17837g7691g7389g2499g14033g1262g19283g14752g709g727g993g16213g4570g14406g16901g7621g1457g4396g3324g13543g1926g11428g8981g2172g11468g7477g1226g991g712g3926g7536g8981g2172g11468g1025g2559 g7389g13543g1926g11428
26、g712g1820g1100410g1505g7621g1319g12227g11352HPLCg13435g8712g9177g8939g14406g16901g7621g708g14279g4581g2559g73895 g1069g14108g709g712g9994g2530g6454g990100 g1069g14108g1457g4396g712g4570g7621g4388g1016g12483g11352g3593g3848g6331g13051g1209g19462g8502g7621g5214g5190g9100g452 g16840g7138g726g3926g7536g
27、14406g16901g7621g1363g11004g17819g2559g7389g11014g18252g6122g11014g18252g19145g12573g11352g8981g2172g11468g712g1000g1363g11004g2530g7378g11004100 g1069g14108g1926g8939g6122g1457 g4396g712g2029g16825g7621g4388g1889g11004g11014g18252g1319g13007g11352g8981g2172g11468g7114g2029g19668g16213g17751g19283g11352g7114g19400g7481g17810g2052g5191g15925g452
