FACSCantoII中文培训手册.pdf

1、 BD FACSCanto II TM 中文培训手册 FACSCantoIIg1117g7095g3625g16861g6267g1980 2 目 录 FACSCanto(II)培训所需设备/试剂 3 FACSCanto(II)开机程序4 FACSCanto(II)关机程序6 BD FACSCanto临床软件进行仪器日常质控（QC） 7 HLA-B27校准9 FACSCanto Clinical六色免洗淋巴细胞亚群检测 10 FACSCanto Clinical软件的获取分析的具体操作 11 FACSCanto Clinical 软件进行HLA-B27分析 14 Diva软件进行HLA-B27

2、手动获取和分析 18 Calibrite四色小球模拟细胞 演示四色实验样本获取和分析 20 Diva软件进行单色&双色获取和分析 23 Cytometer Setup and Tracking（CS&T）操作流程 24 FACSCanto(II)流式细胞仪HTS操作规程 31 FACSCanto(II)日常保养和维护33 FACSCantoIIg1117g7095g3625g16861g6267g1980 3 FACSCanto(II)培训所需设备/试剂 g20g17 g7326g17994g1406g17999g12267g5619g7530g708g4558g20668g16305g881

3、8g1130g22g19g19g74g15g11560g5507g1214g20g19g19g19g794g20g24g19g19g85g83g80g712g2591g16947g17820g17999g16947g7206g19492g712g14125g12267g5619g20g21g91g26g24g80g80g11444g24g80g79g16901g12753g12g727g3g3g21g17 g3370g4554g6214g2591g16947g14514g11444g2256g7783g3224g2554g11560g5316g2256g7783g3940g14613g5282

4、g726g3g21g19g88g79g15g24g19g88g79g15g3g20g19g19g88g79g15g3g21g19g19g88g79g15g3g21g24g19g88g79g712g23g24g19g88g79g712g20g80g79g727g3g3g22g17 g7163g9169g9255g2352g3224g727g3g3g23g17 g10007g7583g712g18431g12674g6214g12331g9186g12753g712g1081g1114g9269g9009g11444g10731g10931g6214g3713g7113g5206g11526g16

5、901g2162g11046g11g24g19g16g20g19g19g80g79g12g712g1072g1114g7941g14498g11046g712g9535g7111g712g9492g13544g727g3g3g24g17 g22g19g19g11550g17911g9492g4716g21961g13697g727g3g3g25g17 g2591g7022g13726g20g21g91g26g24g80g80g11444g24g80g79g16901g12753g11444g16901g12753g7654g21g713g22g1114g727g3g3g26g17 g51g37

6、g54g3g9446g9186g2748g15080g20415g8804g726g19g17g21g21g80g9492g14284g17911g9492g727g3g3g27g17 g49g68g38g79g50g726g2102g7616g13535g712g7481g7032g8799g9091g5334g20g19g8g727g3g3g28g17 g8703g3929g19760g16305g1072g12753g21g80g79g11444g6343g2061g11444g1258g15984g7783g7516g3g11g7472g4013g7263g40g39g55g36g16

7、g46g22g6343g2061g12g727g3g3g20g19g17g3914g13962g11106g18379g452g3g3g195721g726 PBSg18301g2150g7145g8965g726800mlg15080g20415g8804g1117g9446g16403NaCl 8.0gg727KCl 0.2gg727Na2HPO4 1.44gg727KH2PO4 0.24gg727g16947g14514pH g21447.2g7947.4g708g11096HClg6214NaOHg16947g14514g712g2612g3424g15080g20415g8804g1

8、1444g18344g12001g5334g1085g2620g709g712g2256g15080g20415g8804g4554g4585g143711Lg7120.22g173mg9492g14284g17911g9492g2622g712g4564g9305g1549g4488g452 g195722g726 g3914g13962g11106g18379g18301g2150g7145g8965g726g17246g4488g9186g11096PBSg18301g21504%g3914g13962g11106g18379g9446g9186g712g5557g4540g1944g9

9、446g16403g2622g708g9446g16403g7206g19760g16305g1085g7133g6709g6396g712g2256g10013g11444g9305g5334g4671g121450g263g15g2591g2256g1941g20g49g11444g49g68g50g43g9446g9186g2256g17999g9446g16403g709g712g16947g7076PHg1644g34167.27.4g712g5286g110960.22mg11444g9492g14284g17911g9492g2622g13726g12142-8Cg1549g44

10、88g452g1455g11096g7206g712g11096PBSg12336g18426g11302%g9091g5334g11444g5316g11096g9186g452g5316g11096g91862-8Cg4488g7022g712g2591g1455g11096g1072g2712g452 g3g3g3g3g3g3g3FACSCantoIIg1117g7095g3625g16861g6267g1980 4 FACSCanto(II)开机程序 1g451 g6275g5424g12387g2491g3224g11109g9408g712g6275g5424g1405g1214g

11、1306g3224g5142g1495g11444g5739g11109g9408g712g4662g2620g7206g5424g2655g1306g3224g451g9186g9073g17814g1301g2554g9712g1913g11109g9408g452 2g451 g2655g2264g16849g12743g7530g712g3416g2090g10720g11444g11435g19574g4649g16909g7798g1117g712g17859g1941g11096g6247g2621g2748g4598g11825g712g9961g2091g256OKg257g

12、452g452 g16370g4623g7804g19858g2595g1083g16386g13099g13583g7206g19492g7153g11444g13697g13580g3374g7735g1117g7481g1072g1114g7278g12138g1306g3224g1086g11109g14145g5154g17934g6613g452 3g451 g17920g15996g17823g1318g712g2556g2091g7804g19858g1082g11444g5659g6567g7145g5439g452 BD FACSCantog1124g5306g17823g

13、1318 BD FACSDivag17823g1318 4g451 g3416BD FACSDivag17823g1318g2090g10720g11444g11435g19574g4649g16909g7798g1117g712g7184g19760g17859g1941g11096g6247g2621g2748g4598g11825g712g11556g6613g9961g2091g256OKg257g727 g3416BD FACSCantog1124g5306g17823g1318g2090g10720g11444g11435g19574g4649g16909g7798g1117g71

14、2g17859g1941g4598g11825g256BDIS”g712g9961g2091g256OKg257g17931g1941g17823g1318g452 5g451 g3416g256g1306g3224g7798g257g1117g11934g16852g17823g1318g5154g13567g2748g1306g3224g11560g17934g6613g727 g4018g7628g7481g5621g16305g712g17977g6425CytometerConnectg727 6g451 g3416g256g1306g3224g7798g257g1117g7701g

15、11579g9186g19858g8804g5283g712g17911g1406g9186g19858g2748g5154g9489g5327g9186g7278g12138g13522g14498g452 FACSCantoIIg1117g7095g3625g16861g6267g1980 5 7g451 g17977g6425 CytometerFluidics Startup g5424g2655g9186g17439g712g2090g10720g7278g12138g17931g12347g11444g4649g16909g7798g452g9186g17439g2655g2264

16、g4540g8709g708completeg709g2622g712g9961g2091g256OKg257g452 g3416g9186g17439g2655g2264g11444g17911g12347g1117g712g1085g16305g7022g1082g7783g2801g12753g712g2646g2121g9269g9031g9186g2748g5327g9186g1354g17924g9073g1941g7783g2801g12753g452g9186g17439g2655g2264g12347g5311g1354g12331g2539g12753g18051g1117

17、g1955g7530g9186g712g11096g19912g9186g1299g7471g452 8g451 g9712g1913g20148g10013g712g4540g6208g2622g712g1354g3416g256g1306g3224g7798g257g11444g5317g18200g7278g12138g256system readyg257g708g13099g13583g2038g3895g4540g8709g709g452 9g451 g6275g5424g9073g2264g4564g11444g19480g712g7920g7701g9073g2264g4564

18、g7481g8913g7481g8772g8977g452 g6594g19604g9073g2264g4564g1117g11444g8772g8977g712g17977g6425Cytometer Cleaning Modes De-gas Flow Cellg712g2090g10720g7278g12138g17931g12347g11444g4649g16909g7798g452 g7920g7701g17929g18428g7481g8913g7481g8772g8977 g19912g9186 g5327g9186 g1955g7530g9186 g9269g9031g9186

19、 FACSCantoIIg1117g7095g3625g16861g6267g1980 6 g9961g2091g256OKg257g712g7920g7701g9073g2264g8848g712g14613g17928g7481g8772g8977g712g2591g18429g3901g1082g17952g6909g1420g452 10g451g1306g3224g2591g1301g5424g4091g5141g1420g452 FACSCanto(II)关机程序 1g451g3416browserg1117g6455g4554g1323g5951g1072g12753tubeg7

20、12g7783g7516g12753g1117g11531g74810.5%-1%g8529g8799g18344g19152g712g20744g17999g17409g77835ming452 2g451g6546DIg8804g712g20744g17999g17409g7783g14371g46735ming712g10086g2622g2566g1083g7783g7516g12753 3g451g17977g6425 CytometerFluidics Shutdowng727 g17920g15996g256g1955g19485g9186g17439g257g12347g531

21、1g4662g6256g7481g12753g17439g1117g11444g19912g9186g6594g2090g712g5286g11096g9269g9031g9186g2748g1955g7530g9186g9269g9031g17929g1227g12753g18051g452g8596g6909g1420g4662g18095g1917g9186g1411g12753g18051g11105g1214g11520g11444g13571g7334g13884g3685g3726g452 4g451g2090g10720g13099g13583g2591g1301g1955g1

22、9485g11444g6656g12138g2622g712g9961g2091g256OKg257g727 5g451g17968g2090g17823g1318g712g1955g19485g16849g12743g7530g727 6g451g1955g19485g1306g3224g5739g11109g9408g727 7g451g1955g19485g12387g2491g11109g9408g452 FACSCantoIIg1117g7095g3625g16861g6267g1980 7 BD FACSCanto临床软件进行仪器日常质控（QC） g3g7184g16874g341

23、6g37g39g3g41g36g38g54g38g68g81g87g82g1124g5306g17823g1318g6214g13877g7263g3416g37g39g3g41g36g38g54g39g76g89g68g17823g1318g1117g17920g15996g7783g7516g712g18221g19760g16305g1455g11096g37g39g3g41g36g38g54g38g68g81g87g82g1124g5306g17823g1318g7573g17920g15996g14362g2264g2374g16878g13726g2748g52g38g452g3g

24、2137g11096g37g39g3g41g36g38g54g3g26g14498g16878g13726g5598g10803g712g4649g7920g9083g3224g11109g2491g17931g15996g16947g14514g712g6330g16878g13726g5598g10803g16947g7076g3416g20148g4554g11444g19878g1644g1405g13726g712g4649g7920g9083g9893g7039g5334g1644g17931g15996g9083g18431g712g5286g1092g4649g14743g19

25、13g16021g1711g1644g17931g15996g16849g12743g5286g1549g4488g14743g1913g16021g1711g11444g17268g7113g452g1455g11096g37g39g3g41g36g38g54g38g68g81g87g82g1124g5306g17823g1318g1117g11444g47g72g89g72g92g16g45g72g81g81g76g81g74g86g2255g14125g712g6457g7206g19492g17965g17498g5286g11521g9083g9073g5439g13558g1409

26、4g1306g5719g14125g712g11145g1083g2546g7072g11444g2568g2374g2748g17339g2287g452g3g20g714 g2038g3895g37g39g3g41g36g38g54g3g26g14498g16878g13726g5598g10803g726g2566g1072g2586g37g39g3g41g36g38g54g3g26g14498g16878g13726g5598g10803g7783g7516g12753g712g2256g12336g18426g9186g14371g2155g5334g13551g708g13526g

27、20g80g79g709g712g1909g2102g9255g2352g452g3g21g714 g6457g8703g7189g5424g7530g12347g5311g6275g5424g9073g5439g13558g14094g1306g1131g7530g2748g11109g14145g712g6275g5424 g41g36g38g54g3g38g68g81g87g82 g1124g5306g17823g1318g5286g11435g19574g712g2655g2264g9186g17439g452g3g22g714 g17977g6425g256g38g92g87g82g

28、80g72g87g72g85g708g9073g5439g13558g14094g1306g709g257g279g256g54g72g87g88g83g708g16878g13726g709g279g54g87g68g81g71g68g85g71g3g54g72g87g88g83g708g7735g2038g16878g13726g709g452g3g23g714 g3416g256g54g72g87g88g83g3g47g82g87g3g44g81g73g82g85g80g68g87g76g82g81g11g16878g13726g6313g2599g1553g5791g12g257g46

29、49g16909g7798g1117g9961g2091g256g49g72g90g3g47g82g87g3g44g39g708g7136g6313g2599g709g257g452g3g3g24g714 g17859g1941g11560g5316g5598g10803g6313g2599g2554g3937g7032g7503g712g5286g9961g2091g256g50g46g257g452g3g3g25g714 g6457g10135g37g39g3g41g36g38g54g3g26g14498g7761g2038g5598g10803g7735g12718g1082g6256g

30、7278g12138g11444g1553g5791g17859g1941g6214g7920g7701g47g82g87g3g44g39g11g6313g2599g12g451g87g68g85g74g72g87g86g11g19878g1644g12g2554g86g83g72g70g87g85g68g79g3g82g89g72g85g79g68g83g3g73g68g70g87g82g85g86g11g1913g16993g2576g2256g3344g4480g12g712g17859g1941g4540g8709g2622g9961g2091g256g49g72g91g87g257g

31、452g3g26g714 g1306g3224g7278g12138g54g68g89g72g3g54g72g87g88g83g3g37g72g68g71g3g47g82g87g3g44g81g73g82g3g11g1549g4488g7761g2038g5598g10803g6313g2599g1553g5791g12g4649g16909g7798g712g9961g2091g256g60g72g86g257g3FACSCantoIIg1117g7095g3625g16861g6267g1980 8 g27g714 g17977g6425g256g53g88g81g3g86g72g87g8

32、8g83g3g76g81g3g48g68g81g88g68g79g3g80g82g71g72g11g6267g2264g6295g15996g7761g2038g12g257g712g9961g2091g256g49g72g91g87g257g452g3g28g714 g1306g3224g7278g12138g4018g1083g6656g12138g7798g712g1909g2102g6775g2352g5598g10803g7783g7516g12753g712g4662g5327g9186g2664g5445g14322g6616g2144g7472g5142g17897g712g1

33、082g7783g712g9961g2091g256g50g46g257g452g3g3g3g3g3 g3g20g19g714 g7761g2038g17911g12347g14362g2264g17931g15996g712g5557g17823g1318g2090g10720g4018g1083g6656g12138g7798g7206g712g2566g1083g7783g7516g12753g452g3g3g3g3g3 g3g20g20g714 g4018g7761g2038g6208g2255g712g1306g3224g7278g12138g256g54g72g87g88g83g3

34、g38g82g80g83g79g72g87g72g71g3g54g88g70g70g72g86g86g73g88g79g79g92g11g6208g2255g4540g6208g7761g2038g12g257g452g17823g1318g4662g6357g2682g1301g51g39g41g7788g5439g6457g4018g1083g17439g5556g2554g7095g1318g2621g14362g2264g1549g4488g708g38g29g63g51g85g82g74g85g68g80g3g41g76g79g72g86g63g3g37g39g3g41g36g38g

35、54g38g68g81g87g82g3g54g82g73g87g90g68g85g72g63g3g54g72g87g88g83g53g72g83g82g85g87g86g63g3g54g72g87g88g83g53g72g83g82g85g87g66g92g92g92g92g48g48g71g71g66g75g75g80g80g17g83g71g73g709g452g9961g2091g256g41g76g81g76g86g75g11g4540g6208g12g257g712g7761g2038g13571g7567g452g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g3g

36、3FACSCantoIIg1117g7095g3625g16861g6267g1980 9 运行HLA-B27校准 g20g714g16901g2162g2038g3895g726g3g2566g43g47g36g16g37g21g26g16901g2162g11522g1117g11444g43g47g36g16g37g21g26g3g70g68g79g76g69g85g68g87g76g82g81g3g69g72g68g71g86g16901g2162g712g9255g2352g712g2256g1108g9508g2144g16117g7481g20g80g79g19912g9186g

37、11444g16901g12753g1117g712g9255g2352g712g5557g9083g452g3g3g21g714g41g36g38g54g38g68g81g87g82g1124g5306g17823g1318g1082g7530g7920g9083g43g47g36g16g37g21g26g11444g1959g1411g8597g20692g3g3416g17920g15996g43g47g36g16g37g21g26g7761g2038g2173g1306g3224g5621g20139g6208g2255g17994g17911g37g39g3g41g36g38g54g

38、3g26g14498g5598g10803g11444g7761g2038g1092g1085g17333g17911g21g23g4671g7206g452g3g20g714 g6275g5424g41g36g38g54g3g38g68g81g87g82g3g1124g5306g17823g1318g712g3416g38g92g87g82g80g72g87g72g85g14860g2437g1083g17977g6425g54g72g87g3g88g83g712g3416g54g72g87g88g83g2595g1495g2090g10720g11444g1083g6393g14860g2

39、437g1117g17977g6425g43g47g36g16g37g21g26g3g54g72g87g88g83g712g2090g10720g4018g1083g43g47g36g16g37g21g26g7761g2038g4649g16909g7798g452g3g3g3g3 g3g21g714 g3416g2595g1495g7798g1117g17859g1941g8595g11934g11444g43g47g36g16g37g21g26g3g47g82g87g3g44g39g1644g451g54g88g73g73g76g91g1644g2748g37g72g68g71g86g3g

40、47g82g87g3g44g39g1644g451g54g88g73g73g76g91g1644g452g17929g1227g2546g7072g2102g2139g2019g4554g1206g41g47g20g3g51g48g55g11444g7735g2038g2748g71g72g70g76g86g76g82g81g3g80g68g85g78g72g85g11444g1405g13726g452g4649g1214g13571g7628g14371g1955g18429g16305g712g1085g2591g5428g19273g452g9961g2091g54g87g68g85g

41、87g712g2090g10720g4018g1083g4649g16909g7798g712g6267g2264g1082g7783g2622g9961g2091g50g46g452g3g3g3g3g3g3g3 g3g22g714 g1306g3224g5424g4091g14362g2264g7761g2038g41g44g55g38g11109g2491g712g4540g6208g2622g1306g3224g1354g3416g12487g2579g5317g18200g2090g10720g1072g15996g6656g12138g726g54g72g87g88g83g3g70g

42、82g80g83g79g72g87g72g71g3g86g88g70g70g72g86g86g73g88g79g79g92g452g17823g1318g4662g6357g2682g1301g51g39g41g7788g5439g6457g4018g1083g17439g5556g2554g7095g1318g2621g14362g2264g1549g4488g708g38g29g63g51g85g82g74g85g68g80g3g41g76g79g72g86g63g3g37g39g3g41g36g38g54g38g68g81g87g82g3g54g82g73g87g90g68g85g72g

43、63g3g54g72g87g88g83g53g72g83g82g85g87g86g63g3g43g47g36g37g21g26g66g92g92g92g92g48g48g71g71g66g75g75g80g80g17g83g71g73g709g452g3g23g714 g9961g2091g54g68g89g72g712g1549g4488g7761g2038g13571g7628g2467g1306g3224g16878g13726g7569g1318g452g17823g1318g4662g43g47g36g16g37g21g26g7761g2038g13571g7628g14362g22

44、64g1549g4488g3416g3g38g29g63g51g85g82g74g85g68g80g3g41g76g79g72g86g63g38g82g80g80g82g81g3g41g76g79g72g86g63g37g39g63g54g72g87g88g83g3g53g72g86g88g79g87g86g63g43g47g36g16g37g21g26g17g82g83g87g17g3g24g714 g9961g2091g41g76g81g76g86g75g712g7761g2038g13571g7567g452g3g3g3FACSCantoIIg1117g7095g3625g16861g6

45、267g1980 10 FACSCanto Clinical六色免洗淋巴细胞亚群检测 g463g1131g16305g16901g2162g464g726 g7081g709g3 g6343g1411g726CD3/CD8/CD45 /CD4g727CD3/CD16+56/CD45 /CD19g3339g14498g14743g1913g6343g1411g452 g7082g709 FACS Lysing Solutiong708g9446g15984g13136g709g70810g104,g1455g11096g2173g11096g15080g20415g8804g12336g1842

46、6g62081g104g709g452 g463g4558g20668g8597g20692g464g726 1g451 g3416g9073g5439g12753g1117g2256g194150ulg1909g2102g9255g2352g11444g6343g2061g1944g15984g452 2g451 g2256g1941 20ul CD3/CD8/CD45/CD4/CD16+56/CD19g712g9169g7163g9255g2352g712g4564g9305g18095g1913g7022g13726 15g71320ming452 3g451 g2566g2090g22

47、56g1941450ul 1g104FACS g9446g15984g13136g712g1909g2102g9255g2352g712g18095g1913g7022g1372615ming452 4g451 24g4671g7206g1973g1082g7530g11096FACSCanto Clinicalg17823g1318g14823g25662500g1114g9211g5156g13558g14094g17931g15996g7920g9083g712g1082g7530g2173g5316g1909g2102g9255g2352g452g727 g463g8984g5951g

48、1211g20137g464g726 1. g7783g7516g1455g11096EDTAg6343g2061g1944g15984g712g4564g9305g7022g13726g71224g4671g7206g1973g3892g10806g712g3892g10806g2173g1909g2102g9255g2352g452 2. g7683g14498g2748g9446g15984g8597g20692g5316g3416g4564g9305g18095g1913g17931g15996g712g5286g1909g2102g9255g2352g17911g12347g1117

49、g712g4717g18431g19554g8594g15984g7783g11444g20238g9397g712g1301g1917g15984g7783g11145g3416g12753g3825g1082g452 FACSCantoIIg1117g7095g3625g16861g6267g1980 11 FACSCanto Clinical软件的获取分析的具体操作 g708g20g709g451g6295g15996g41g36g38g54g38g68g81g87g82g5424g7530g12347g5311g712g3416g7804g19858g1082g9961g2091 g17931g1941g41g36g38g54g38g68g81g87g82g38g79g76g81g76g70g68g79g17823g1318g452g3g708g21g709g451g3416g2090g10720g11444g256g47g82g74g74g76g81g74g3g44g81g2