1、花柱染色观察1 取花柱于 25%的冰醋酸中固定;2 80 度,20 分钟; 3 Anilineblue 染色4 绿光下观察.1 Fix styles in FAA for 24hr or more;2 After rinse in tap water (treat styles in a nearly saturated aqueous solution of NaOH(8N)for 8-24hror cleared by autoclaving in 10%(w/v) aqueous sodium sulphite(is pi,104kpa) for 15min;3 Transfer the
2、 softened styles to a small beaken of tap water for one or more hours to remove most of the sodium hydroxide(sodium sulphite);4 Stain the styles in a 0.1% solution of aniline blue dye in 0.1N K3PO4 for 1-24 hr(adequate staining can also be done in a small amount of the dye on a glass slide);5 For ob
3、servations the styles are mounted in a few drops of the staining medium on clean glass slides and are covered with cover slips;6 Observe the styles by directing illuminating the slide with UV of 356 nm in a darkroom.FAA :( 10ml ) Formalin 1ml + 80%Alchol 8 ml + Acetic acid 1 mlAniline Blue: 0.1% aniline blue dissolved in 0.1N K3PO47g(3.5g)/L tribasic potassium phosphate 1g/L aniline bluedissolve in a small quantity of distilled water and mix the solutions and make up to 1 liter.10% sodium sulfite: 10g of sodium sulfite in 100ml of water8N NaOH: 32g of NaOH in 100ml of water
