1、一、 pGADT7(Amp)酵母表达载体(AD-Amp)AD forward primer: 5-TAATACGACTCACTATAGGGC-3AD reverse primer: 5-AGATGGTGCACGATGCACAG-3F：GCAT CGA TAC ATG GC T TG T GCTACTCTGAR：GGCCGGATCCTTAAGAGAGGTAGCTGGGAGAD forward primer: 5-TAATACGACTCACTATAGGGC-3AD reverse primer: 5-AGATGGTGCACGATGCACAG-3二、 pGBKT7(Kan0 酵母表达载体(BD-Ka
2、n)三、BIFC(Kan)验证互做蛋白表达载体（德国）四、 pGR107(Kan)表达载体（PVX-10kb）吴建国MCS:ClaI/SmaI/SalI(pgR107)MCS:ClaI/AscI/NotI/SalI(pgR106)五、 BIFC(Amp)载体（美国）3075（nEYFP-329bp）3076 (cEYFP-218bp)F：GGCGAATTC ATG GCTTGTGCTACTCTGAR：GGCCCTAGGAGAGAGGTAGCTGGGAGTAA TAG TGA六、 RNAI(Kan)载体 -pFGC5941（王宗华）七、pGEX-4T-1(Amp)原核表达载体八：pET-29a(
3、Kan)原核表达载体九、. pEGAD(Kan)植物表达载体*Xba cleavage blocked by overlapping dam methylation. Must grow in dam-strain to cut. Note, Xho is not unique.pTRV2(a) Genome organization of TRV. The TRV-RNA1 open reading frames(ORFs) correspond to 134 and 194 kDa replicase, movement protein (MP)and a 16-kDa cysteine-
4、rich protein. The TRV-RNA2 ORFs corresponds tocoat protein (CP), and the 29.4 and 32.8 kDa proteins. gRNA, genomicRNA; sgRNA, subgenomic RNA. Asterisks (*) indicate the readthrough of134 kDa protein.(b) TRV based VIGS vectors. TRV cDNA clones were placed in betweenthe duplicated CaMV 35S promoter (235S) and the nopaline synthaseterminator (NOSt) in a T-DNA vector. LB and RB refer to left and rightborders of T-DNA. Rz, self-cleaving ribozyme. MCS, multiple cloningsites.十、十一、1103-YFP十二：pBINPLUS十三、pBin43830773078
