1、刚好我最近也在养这个细胞，试着做下解答。根据 ATCC 的介绍，这个细胞是贴壁生长的（我观察觉得是半贴壁的） ，传代的时候不要用胰酶消化，而是直接用细胞刮刀刮下，然后吹散成单个细胞即可。以下是 ATCC 的详细说明，希望能对你的细胞培养有所帮助。Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbeccos Modified Eagles Medium, Catalog No. 30-2002. To make the complete growt
2、h medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Atmosphere: air, 95%; carbon dioxide (CO2), 5% Temperature: 37.0C Subculturing: Protocol: Subcultures are prepared by scraping.For a 75 cm2 flask, remove all but 10 ml culture medium (adjust
3、 amount accordingly for other culture vessels). Dislodge cells from the flask substrate with a cell scraper; aspirate and add appropriate aliquots of the cell suspension into new culture vessels. Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended Medium Renewal: Replace or add medium every 2 to 3 days
