1、小鼠 Hedgehog 相互作用蛋白 (HHIP)酶联免疫吸附测定试剂盒使用说明书产品编号：E-EL-M0624 （本试剂盒仅供体外研究使用、不用于临床诊断!）声明：尊敬的客户，感谢您选用本公司的产品。本产品选用世界著名生产厂家的原料，采用专业 ELISA kit 生产技术制造。适用于体外定量检测小鼠血清、血浆、组织匀浆或细胞培养上清液中天然和重组 HHIP 浓度。使用前请仔细阅读说明书并检查试剂组分！如有疑问，请及时联系伊莱瑞特生物科技有限公司。试剂盒组成：名称-中文 名称-英文 规格 保存条件ELISA 酶标板 Micro ELISA Plate 812 / 86* 4冻干标准品 Refe
2、rence Standard 2/ 1 支* 4标准品 Incorrect assay temperature Use recommended incubation temperature. Bring substrate to room temperature before use.Inadequate reagent volumes Low signalImproper dilutionCheck pipettes and ensure correct preparationVerify the wavelength and filter setting in the plate read
3、er.Deep color but low value Plate reader settings not optimal Open the Plate Reader ahead to pre-heatLarge CV Inaccurate pipetting Check pipettesConcentration of detector too high Use recommended dilution factor. Plate is insufficiently washed Review the manual for proper wash. If using a plate wash
4、er, check that all ports are unobstructed.High backgroundContaminated wash buffer Make fresh wash bufferImproper storage of the ELISA kit Stop solutionAll the reagents should be stored according to the instructionsLow sensitivityStop solution not added Stop solution should be added to each well befo
5、re measurementDeclaration:1. Limited by the current conditions and scientific technology, we cant completely conduct the comprehensive identification and analysis on all the raw material provided. So there might be some qualitative and technical risks for users using the kit.2. The final experimenta
6、l results will be closely related to validity of the products, operation skills of the operators and the experimental environments. Please make sure that sufficient samples are available.3. To get the best results, please only use the reagents supplied by the manufacturer and strictly comply with the instructions in the description!4. Valid period: 6 months.5. This description is also suitable for 48T kit.
