1、STR检测试剂盒,肖怀东 Field Application Specialist Tel: 8008208982; 4008208982 E-mail: CNAPM,Workflows for Casework,Sample Collection,DNA Extraction,Amplification,Detection & Anslysis,AmpFSTR PCR Amplification Kit,GeneAmp PCR System 9700 Cycler,AutoMate Express Forensic DNA Extraction System,PrepFiler and Pr
2、epFiler BTA Kit,310/3130/3130xl Genetic Analyzer 3500/3500xL Genetic Analyzer,GeneMapper ID 3.2.1, 3.3, X Software,4N6FLOQSwabs,Veriti 96-Well Thermal Cycler,An end-to-end integrated solution.,Workflows for Database,Sample Collection,Sample Preparation,Amplification,Detection & Anslysis,310/3130/313
3、0xl Genetic Analyzer 3500/3500xL Genetic Analyzer,GeneMapper ID 3.2.1, 3.3, X Software,Veriti 96-Well Thermal Cycler,Buccal DNA Collector,Prep-n-Go Buffer,Applied Biosystems Direct PCR Amplification Kits,NUCLEIC-CARD,4N6FLOQSwabs,An end-to-end integrated solution.,Casework Kits,2001,2007,2009,2010,2
4、012,Identifiler Kit,GloberFiler Kit,Sinofiler Kit,MiniFiler Kit,Identifiler Plus Kit,First commercially available 6-dye amplification kit,NGM SElect Kit,NGM Kit,2013,Yfiler Plus Kit,Direct Amplification Kits,2009,2010,2011,2012,2013,Identifiler Direct,GloberFiler Express,YFiler Direct,Huaxia,Prep-n-
5、Go Buffer,Fast & Direct Amplification Coming soon!,Treated Paper Untreated Paper Buccal swabs,First commercially available 6-dye amplification kit,Identifiler/ Identifiler Plus/Identifiler Direct,PCR Conditions,Total Time: 3hrs 30 min,Total Time: 2hrs 35 min,Time savings: Approximately 1 hour,Total
6、Time: 2hrs 20 min,ID_28cycles，10uL体系，1uL模板,ID Plus_28cycles，10uL体系，1uL模板,ID plus_29cycles，10uL体系，1uL模板,ID Direct,Sinofiler and Huaxia,PCR Conditions,Total Time: 3hrs 30 min,Time savings: Approximately 1 hour,Total Time: 2hrs 20 min,Huaxia,* Green loci are required for Codis and red loci are optional
7、 for Codis.,GlobalFiler and GlobalFiler Express,GlobalFiler and GlobalFiler Express,GlobalFiler Kit PCR Reaction Protocol,Protocols developed on Veriti thermal cyclers using 100% ramp speed,Performance verified on the 9700 thermal cyclers using MAX mode,PCR Conditions95C/ 1m94C/ 20s59C/ 90s60C/ 10m,
8、29,Total 80 min,GlobalFiler Express Kit PCR Reaction Protocol,Protocols developed on Veriti thermal cyclers using 100% ramp speed,Performance verified on the 9700 thermal cyclers using MAX mode,PCR Conditions95C/ 1m94C/ 3s60C/ 30s60C/ 8m,25-27,Total 40 min,Prototype GlobalFiler Kit vs. Identifiler P
9、lus Kit,Identifiler Plus Kit: 50 RFU PAT (3130xl) GlobalFiler Kit: 175 RFU PAT (3500xL),Identifiler Plus Kit: Partial Profile (21 alleles),GlobalFiler Kit: Partial Profile (28 alleles),Bone Sample; Organic Extraction,An Unprecedented Power of Discrimination,Minifiler kit,专门针对困难检材设计补充现有试剂盒 Identifile
10、r / Identifiler Plus /Sinofiler/ GlobelFiler/NGM,显著提高困难样本分析成功率！!,MiniSTR 引物设计,STR repeat region,miniSTR primer,miniSTR primer,Conventional PCR primer,Conventional PCR primer,Smaller PCR products work better with low copy number or fragmented DNA templates,Allele Size Range of miniSTR Amplicons Compa
11、red to Identifiler,Average 58% reduction in size of smallest allele and 48% for the largest alleles excluding D21 (16%),ID amplification of blood on denim (14 wks),MiniSTR kit of blood on denim (14 wks),Enhanced Discrimination Power on Degraded Samples,10 STR Markers Under 220 bp,GlobalFiler Kits 10
12、 mini-STRs 220 bp PI* = 9.2 x 10-12,1,2,3,4,5,6,7,8,9,10,Enhanced Discrimination Power on Degraded Samples,MiniFiler Kit 8 mini-STRs 220 bp PI* = 1.05 x 10-10,Enhanced Discrimination Power on Degraded Samples,Comparison of Pi Values,YFiler and YFiler Direct,YFiler Direct,综合说明,反应体系,PCR反应参数,循环参数随实验室具体情况而定,谢 谢!,
