1、2008, 17( 2)ys k FujianAnalysisrAM E; J ms |: O657. 72 DS M : A cI|: 1009- 8143( 2008) 02- 0057- 04Determination ofcyclamate in foodwithHigh PerformanceLiquidChromatography(HPLC)XieLingyang, ZhangQingchun, WangYuanyuan(ChinaNationalCenter forQuality Supervision and Test of Standard parts, Haiyan 314
2、300, China)Abstract: Thismethod is using thederivativeN, N- D ichlorocyclohexylam ineproduced by cyclamatew ith sodium hypochlo-rite in a strong acid environment and extracted with n- hexane, which has higherUV response, to determine cyclamatew ith high performance liquid chromatography(HPLC). The r
3、esults show that it is suitable fordeterm ination of cyclamate infood.Keywords: cyclamate; HPLC; foodS,- )(C )1986M T T J F4, “ - SW PC4bSE? J C Kv P 0. 65g/kg, ! J C4,C50- 60, O gz,N,;SEW P,1960 My T, M10$FDA,Sg 1970 MbV A,- )(C ) ?-b SSE SSES(NRC /NAS) 1986 M V ?5,# S EE? ?“F J “( 21CFR, k189. 135
4、, 1994)b ,- )(C )y$ JF4 PS,# J Pb “ -S =S_ZE M Ea1 E Eb TN a y a e, _ C ,y%-?a- 3LT,7 ZE5_ a # J C , OZEea y a_K, ? _1 pb1 实验部分1. 1 N k4S (Agilent) 1200rAM N,_ bY( B),J?( B),-( B),B ( qv18M8 ), ; ,Q o ), _ ),C S A( 1000Lg/mL): P HB d iS PAbl : 2007- 8- 22Te: 2( 1979 ), o, =,V Y J a aF4_TbE- mai:l xi
5、elingyang 163. net57ys k / 2008, 17( 2)1. 2 Hq : XDB - C18( 4. 60Lm 150mm, 5Ln);M: :J?= 20: 80; : 1. 0mL/m in;:40e ;o: 314nm;“ : 20LL; = HW, b1. 3 “ ) 1. 3. 1 “ A!%8“ : | (“ 2. 00010. 000 X,F 40 50mLM100L ,bm F 20min, F ,V r“ AbA8“ : ( !(c=“ 5 “ =)b1. 3. 2 3 |1. 3. 1“A2 10mLsA P,GQF 2mL1: 1 ; Aa5. 0
6、0mL-a2mLQ o )(r o5% ), 1m ins,“ MF 25mL5% _ ) A,Q 1min,s“ / A, A r ( 0. 45Lm)V rAM sbC S A) “ b2 结果与讨论2. 1 mm1C S m,m2vh“ FS m,m3 “ FS m,m4“ FS mb(m in)m1 C S m(m in)m2 vh“ FS m582008, 17( 2) 2:rAM E J C m3 “ FS mm4 “ FS m2. 2 S wLB ) dC S A( 1000Lg/mL) 2Lg/mLa10Lg/mLa50Lg/mLa200Lg/mLa600Lg/mL“ A,1.
7、 3. 2) “s, A iC ( Lg/mL)TBm,S wLA= 1. 927C- 7. 372,M1“ R= 0.99992,s1 p,- )(C )2 600Lg/mLS =L zb2. 3 MsY1 Y- aJ?- T M,Y- 1 7: 3aJ?- 1 8: 2 H, ( zs , I nY v, ON ,4J?- (818: 2)T Mb2. 4 “ A!F s L l q,F ; 7/ ,9 V P“ MbsY iQ o ) L, L,F 5%Q o )(r oc v5% ) 2mL V Q bF 5% _ ) A V59ys k / 2008, 17( 2) Hq/ 3=“
8、o #Q o )b2. 5 _K3.1 pC _K1Lg/mLb2. 6 l k |6z“ , (F 20Lg/ mLC S PA, Hq) ,9 MS ,T V1bV1 V,ET zbV1 kT/Lg# mL- 1 (/Lg# mL- 1 RSD /%19. 86 19. 92 19. 7119. 89 19. 94 19. 7519. 84 4. 21 |B “ ,sYF iC S PA, Hq) ,9 l q,T V2bV2 V,C l q98% 101%,V E bV2 l kT/Lg# mL- 1FS /Lg# mL- 1 L/Lg# mL- 1 l q/%0002202001. 9
9、519. 84202. 497. 499. 2101. 22. 7 o- ) 3N, N -= o-o ,210nma314nm) l,210nm) 3 4 M l ,7314nm) aL, 4314nm_ob3 结论Q o )| J C N, N-= o-,-Y |,rAM E,LS z, 2 a # ( , ? SESC 1 pb参考文献1 GB 2760- 1996 J F4 P 3S S.2 GB/T 5009. 97- 2003 J - ) S.3f, +. - 3rAM E J C J. . 2005( 6): 43- 44.4 M, , W.rAM EC J.S S/. 2004(12): 31- 32.60
