1、1Activated carbon to extract of calf serum in the adsorption of peptidesAbstract Objective To study the activated carbon to extract of calf serum in the adsorption of peptides. Methods activated carbon added to the extract of calf serum, the filtered and absorbance detection of protein content, resp
2、iratory activity and amino acids. The results were compared with the non-activated carbon, activated carbon, after adding protein extract of calf serum to the absorbance value, peptide levels were significantly decreased; respiratory activity and amino acid content did not change significantly. Conc
3、lusions activated carbon to extract of calf serum in the peptides are adsorbed effect. Keywords: Activated carbon; calf serum to the protein extract; polypeptide The Absorption Effect of Active Carbon on the Polypeptide in Calf Serum Abstract Protein-freeLI Xin, WANG Tie (Liaoning Ahon Pharmaceutica
4、ls Corporation Limited, Jinzhou 121000 China) Abstract: Objective To study the effects of active carbon on the polypeptide in calf serum 2abstract protein-free. Methods Put active carbon into calf serum abstract protein-free; and determine protein content, absorbance , amino acid content and breath
5、nature after filtration.Result Compared with the group without active carbon, absorbance and protein content decreased, while breath nature and amino acid content did not decrease.Conclusions Polypeptide was absorbed by active carbon Keywords: active carbon; calf serum abstract protein-free; polypep
6、tide Activated carbon is a multi-aperture carbide, a very rich pore structure, has good absorption characteristics, its physical and chemical adsorption by the adsorption forces made their appearance and color was black. The composition of carbon in addition to the main , it also contains a small am
7、ount of hydrogen, nitrogen, oxygen, its external shape of a hexagon, the irregular hexagonal structure, determine its multi-body features orange and high surface area per gram of activated carbon which has more than equivalent to 1 000 m2 surface of many, the adsorption capacity, desorption speed, a
8、cid, alkali, high temperature, non-toxic, tasteless, odorless; widely used in national defense, scientific research, 3petrochemical, biochemical, environmental protection, medicine, food, health and other fields. Almost all synthetic and bio-pharmaceutical bulk drugs, especially medicine were refine
9、d by decolorization activated carbon 1,2. The main role of activated carbon to remove impurities and improve the purity and removal of heat source. Our products calf blood protein extracts used in the production, active carbon, this paper bleaching, while in the calf serum protein extract to the ads
10、orption of peptides and its effect on product quality. 1 Materials and methods 1.1 Materials 1.1.1 Activated Carbon (Shanghai Activated Carbon Factory Co., Ltd.); calf serum to the protein extracts (Austria Hong medicine provided). 1.1.2 Drugs and reagents serum albumin (Pharmaceutical and Biologica
11、l Products); phenol reagent (Academy of Medical Sciences Centre for biological agents); alkaline copper (Austria Hong medicine provided). 1.1.3 Instrument UV-1700 spectrophotometer (Shimadzu Suzhou Instrument Manufacturing); BP211D electronic balance (Beijing Sartorius limited liability company); po
12、rous 4membrane filters. 1.1.4 HPLC 1.1.5 respiratory activity detector 1.2 Methods Take extract of calf serum to the six batches of 1 000 mL, detection of absorbance, protein, amino acid content and respiratory activity (group 1), activated carbon were added to 0.15 g, mix well, place 30 min, filter
13、 In addition to carbon, absorbance detection, protein content, amino acid content and respiratory activity (group 2), then 0.15 g activated carbon were added to repeat the operation twice, too (group 3), (4) .4 set of data compared. 1.3 Detection 1.3.1 Determination of absorbance 3 Based on ultravio
14、let - visible spectrophotometry (”China Pharmacopoeia” -2005 edition Appendix), was measured at a wavelength of 420 nm absorbance values. 1.3.2 Determination of peptide content Preparation of standard curve: precise amount of standard protein solution (0.1 mg / mL) 0mL, 0.2 mL, 0.4 mL, 0.6 mL, 0.8 m
15、L, 1.0 mL, respectively, in the test tube, add water to 1 mL, and then each with alkaline copper solution of 55 mL, and mix in the room temperature 10 min, then add 0.5 mL phenol reagent shake at room temperature for 30 min, at a wavelength of 650 nm with reagent blank to zero, determine the absorba
16、nce values of each tube to protein content abscissa, ordinate is absorbance standard curve. Li Xin, et al: activated carbon to extract of calf serum in the adsorption of peptides of Liaoning Medical College in August 2008, 29 (4) Sample determination: Take 10-fold dilution, as the test solution, pre
17、cise amount of the test solution 1 mL, in the test tube, add alkaline copper solution, 5 mL, shake at room temperature for 10 min, then add phenol reagent Shake 0.5 mL, at room temperature for 30 min, was measured at 650 nm wavelength absorbance, samples obtained from the standard curve of protein c
18、ontent. 1.3.3 Determination of amino acids The control solution and test solution were injected into the liquid chromatograph, each 2.5 L, record the chromatogram, according to internal standard peak area calculation. The formula is: Free amino acid content (mg / mL) = 6(Filter-like concentration -
19、the concentration of internal standard) 58.8 1 000 internal standard concentration 1.3.4 detected by breath test samples in each group of respiratory activity was measured as follows: Pre-wash the manometer reaction flask and dried standby, installed on the piezometric liquid pressure measurement, t
20、he regulate the water level to 150 mm. Add 10% potassium hydroxide solution, 0.2 mL in the reaction flask small cup (to increase the hydrogen K on the carbon dioxide absorption area, in the potassium hydroxide solution into a small piece of filter paper). reaction flask to join the phosphate buffer
21、1.1 mL, liver homogenate 1.0 mL, then add 0.2 mL for the test materials in the reaction flask. As the role of the liver homogenate oxygen itself, so to test instead of 0.2 mL phosphate buffer for the test materials for the blank tube. Will be installed by a good reaction flask and manometer tube con
22、nected to No. (Note closed, without rendering leakage gas) at 37 water bath. starting oscillator 10 min (120 beats / min) outside the reaction flask temperature of the same surface will be transferred to the right manometer 150 mm, surface reading down the left (A) . close the three-piston, start ti
23、me, 30 min reaction of the right 7side of the manometer liquid level raised to 150 mm, surface readings down the left side (B). Repeat for two, write down the readings during the experiment C and D. must add a set of terms for the calibration of the voltage regulator in the reaction flask with the t
24、est solution before adding the same volume of phosphate buffer, to test tubes with the same conditions, observe the pressure change ( C), to eliminate the influence due to temperature and atmospheric pressure caused the error. Links to free paper download Calculation QO2 (lO2/mg h )=( AB) + (CD) K1-
25、 C K2 / (G T) K1: the test or reference substance in the reaction flask constant G: dry weight per mL liver homogenates (105 to constant weight) (mg) T: reaction time (hours) K2: constant voltage meter group reaction flask: C: the pressure regulator and the total group. Stimulation index (SI) = the
26、test QO2 / blank QO2 This product is the stimulation index (SI) should be not less than 3.0. 81.4 Statistical analysis Experimental data to +-s that the application of statistical software SPSS 11.5 was used for univariate analysis of variance and q test, P 0.05 with significant, P 0.01 with a very
27、significant meaning. 2 Results 2.1 deproteinized calf blood activated carbon injection absorbance values of Standard curve shown in Figure 1. Figure 1 Standard curve of protein content of different amounts of carbon added, compared with and without activated carbon, absorbance values were significan
28、tly decreased; The more the addition of activated carbon, the more obvious decline in the absorbance, the first group and the first 2,3 1 group, P 0.01, Group 4 compared with 3 P 0.05, results in Table 1. 2.2 after adding activated carbon injection calf blood protein content of peptides After adding
29、 different amounts of carbon, compared with and without activated carbon, peptide content were significantly decreased; activated carbon added more peptides decreased more significantly, the first 2,3 in group 1 9than group, P 0.01, 4, compared with the first 3 P 0.05, results in Table 1. 2.3 after
30、adding activated carbon injection calf blood protein amino acid content of After adding different amounts of carbon, compared with and without activated carbon, amino acids in each group no significant change was not significant. Results in Table 1. 2.4 after adding activated carbon injection calf b
31、lood protein amino acid content of After adding different amounts of carbon, compared with and without activated carbon, amino acids in each group no significant change was not significant. Results in Table 1. Table 1 Activated Carbon Injection calf blood protein absorbance 3 Discussion Pharmaceutic
32、al companies in the activated carbon as a bleaching agent commonly used adsorbent, is widely used, before we can focus only on activated carbon adsorption of colored material, so that the absorbance value decreased. Our experiments found that: activated carbon added to the protein bovine serum to ex
33、tract, not only to extract the color lighter, the absorbance decreases, the role played bleaching, 10while adsorption of calf serum to the protein extracts in the peptide, so the protein content decreased. but did not affect the amino acid content and respiratory activity. Therefore, through this ex
34、periment suggested that the decolorization using activated carbon, they should choose the appropriate concentration, or may lead to lower protein content. References 1 National Licensed Pharmacist Examination candidate guide. Pharmacy M. Beijing: Chinese Medical Science and Technology Press, 2000:10
35、7. 2 SHEN Zeng-min, Zhang Wenhui, Zhang Xuejun, et al. Activated carbon preparation and application M. Beijing: Chemical Industry Press, 2006:314. 3 National Pharmacopoeia Committee. Pharmacopoeia Appendices of Chinese Pharmacopoeia 2005 edition M. Beijing: Chemical Industry Press, 2005:1 Links to free paper download
