1、pGEX-5X-1 编号! 载体名称! 北京华越洋生物!“#$%)( 氨苄( 备注)! 复制子是)?.( 产品目录号)! 3DM()BHEDJ0=()JB(HI(C0;)=D(NH=J;DC(J)(EH( R(S=(JCD(*:$(:)C(HB(*:$(?J=E(R(S=(0M(.(;=(JCD(*:$(*B0NQ( UFDBD(C0;)=D(NH=J;DC(0BD(04HND(.(;=/(JCD(V04?0EDK(BDCDBNH(4D(JCDM(IHB()BD)0B0E(HI(C0;)=DC( 0MM(*:$(XM(-(;=(1H=J;C(0M(*:$(X(0BD( 0N0(0ED(:D)F
2、0BHCD(4J=L(;DMC(TEF(BDJBDM(4JIIDBCQ( ( )*“+ 系列载体：( ()*“+,.$(“1H_WZ7Y( ()*“+,3$( ()*“+,3$( ()*“+,3$( ()*“+,8+( ()*“+,%$,.( ()*“+,%$,3( ()*“+,%$,8( ()*“+,-+,.( ( )*“+,-+,3()*“+,-+,8( ()*“+,RY,.( ()*“+,RY,3( ()*“+,RY,8( ()*“+,*( ! “#$%( 载体序列! ORIGIN 1 AGCTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCC
3、ATC GGAAGCTGTG 61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT 121 TCTGGATAAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC 181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA 241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC 301 AA
4、CCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC 361 GCGATGAAGG TGATAAATGG CGAAACAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC 421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA 481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC 541 TTGAAGGAGC GGTTTTGGAT
5、ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT 601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AATGCTGAAA ATGTTCGAAG 661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT 721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA 781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCAC
6、A AATTGATAAG TACTTGAAAT 841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC 901 ATCCTCCAAA ATCGGATCTG ATCGAAGGTC GTGGGATCCC CGAATTCCCG GGTCGACTCG 961 AGCGGCCGCA TCGTGACTGA CTGACGATCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA 1021 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCC
7、GGGA 1081 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA 1141 CCCAGTCACG TAGCGATAGC GGAGTGTATA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC 1201 GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT 1261 TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT 1321 ATCCGC
8、TCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA 1381 TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG 1441 TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC 1501 GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG 1561 AAGAACGTTT TCCAATGATG
9、AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC 1621 GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG 1681 TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT 1741 GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG 1801 GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAAC
10、ATGGG GGATCATGTA ACTCGCCTTG 1861 ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC 1921 CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT 1981 CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT 2041 CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG
11、 CGTGGGTCTC 2101 GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA 2161 CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT 2221 CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT 2281 TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA 2341
12、 CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA 2401 AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC 2461 CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG 2521 TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG 2581 GCCACCACTT CAAG
13、AACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC 2641 CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT 2701 TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG 2761 AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC 2821 TTCCCGAAGG GAGAAAGGCG GACAGGTAT
14、C CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC 2881 GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC 2941 ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA 3001 ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT 3061 TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TAC
15、CGCCTTT GAGTGAGCTG 3121 ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG 3181 AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATAAATT 3241 CCGACACCAT CGAATGGTGC AAAACCTTTC GCGGTATGGC ATGATAGCGC CCGGAAGAGA 3301 GTCAATTCAG GGTGGTGAAT GTGAAACCAG TAACGTTATA CGATGTCGCA GAGTATGC
16、CG 3361 GTGTCTCTTA TCAGACCGTT TCCCGCGTGG TGAACCAGGC CAGCCACGTT TCTGCGAAAA 3421 CGCGGGAAAA AGTGGAAGCG GCGATGGCGG AGCTGAATTA CATTCCCAAC CGCGTGGCAC 3481 AACAACTGGC GGGCAAACAG TCGTTGCTGA TTGGCGTTGC CACCTCCAGT CTGGCCCTGC 3541 ACGCGCCGTC GCAAATTGTC GCGGCGATTA AATCTCGCGC CGATCAACTG GGTGCCAGCG 3601 TGGTGGTG
17、TC GATGGTAGAA CGAAGCGGCG TCGAAGCCTG TAAAGCGGCG GTGCACAATC 3661 TTCTCGCGCA ACGCGTCAGT GGGCTGATCA TTAACTATCC GCTGGATGAC CAGGATGCCA 3721 TTGCTGTGGA AGCTGCCTGC ACTAATGTTC CGGCGTTATT TCTTGATGTC TCTGACCAGA 3781 CACCCATCAA CAGTATTATT TTCTCCCATG AAGACGGTAC GCGACTGGGC GTGGAGCATC 3841 TGGTCGCATT GGGTCACCAG CA
18、AATCGCGC TGTTAGCGGG CCCATTAAGT TCTGTCTCGG 3901 CGCGTCTGCG TCTGGCTGGC TGGCATAAAT ATCTCACTCG CAATCAAATT CAGCCGATAG 3961 CGGAACGGGA AGGCGACTGG AGTGCCATGT CCGGTTTTCA ACAAACCATG CAAATGCTGA 4021 ATGAGGGCAT CGTTCCCACT GCGATGCTGG TTGCCAACGA TCAGATGGCG CTGGGCGCAA 4081 TGCGCGCCAT TACCGAGTCC GGGCTGCGCG TTGGTGC
19、GGA TATCTCGGTA GTGGGATACG 4141 ACGATACCGA AGACAGCTCA TGTTATATCC CGCCGTTAAC CACCATCAAA CAGGATTTTC 4201 GCCTGCTGGG GCAAACCAGC GTGGACCGCT TGCTGCAACT CTCTCAGGGC CAGGCGGTGA 4261 AGGGCAATCA GCTGTTGCCC GTCTCACTGG TGAAAAGAAA AACCACCCTG GCGCCCAATA 4321 CGCAAACCGC CTCTCCCCGC GCGTTGGCCG ATTCATTAAT GCAGCTGGCA C
20、GACAGGTTT 4381 CCCGACTGGA AAGCGGGCAG TGAGCGCAAC GCAATTAATG TGAGTTAGCT CACTCATTAG 4441 GCACCCCAGG CTTTACACTT TATGCTTCCG GCTCGTATGT TGTGTGGAAT TGTGAGCGGA 4501 TAACAATTTC ACACAGGAAA CAGCTATGAC CATGATTACG GATTCACTGG CCGTCGTTTT 4561 ACAACGTCGT GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC 4621 C
21、CCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT CCCAACAGTT 4681 GCGCAGCCTG AATGGCGAAT GGCGCTTTGC CTGGTTTCCG GCACCAGAAG CGGTGCCGGA 4741 AAGCTGGCTG GAGTGCGATC TTCCTGAGGC CGATACTGTC GTCGTCCCCT CAAACTGGCA 4801 GATGCACGGT TACGATGCGC CCATCTACAC CAACGTAACC TATCCCATTA CGGTCAATCC 4861 GCCGTTTGTT CCCACG
22、GAGA ATCCGACGGG TTGTTACTCG CTCACATTTA ATGTTGATGA 4921 AAGCTGGCTA CAGGAAGGCC AGACGCGAAT TATTTTTGAT GGCGTTGGAA TT / 其他大肠杆菌表达载体： pBV221 ptdTomato pET-52b(+) pAmCyan pDsRed-Express2 pBV220 pCold-GST pColdS-SUMO pCold TF pCold IV pCold III pCold II pCold I pE-SUMO pCold-ProS2 pBAD102/D-TOPO pBAD202/D-TOP
23、O pACYC184 pBAD/Thio-TOPO pBad/Myc-His C pBad/Myc-His B pBad/Myc-His A pBad/His C pBad/His B pBad/His A pBAD-TOPO pET-23b(+) pET-23a(+) pET-23c(+) pET-23(+) pET-12b(+) pET-12c(+) pET-12a(+) pET-11b(+) pET-11a(+) pET-11c(+) pBad24 pQE-82L pQE-81L pQE-80L pQE-32 pQE-9 pQE-16 pQE-31 pQE-60 pQE-70 pQE-4
24、0 pET-51b(+) pET-50b(+) pET-49b(+) pET-48b(+) pET-47b(+) pET-26b(+) pET-32a(+) pET-21b(+) pET-22b(+) pET-14b pET-16b pET-15b pET-19b pET-20b(+) pET-21d(+) pET-21c(+) pET-21b(+) pET-21a(+) pET-24a(+) pET-24d(+) pET-25b(+) pET-27b(+) pET-28a(+) pET-30a(+) pET-42a(+) pET-43.1c(+) pET-43.1b(+) pET-43.1a
25、(+) pET-44a(+) pET-44c(+) pET-46 EK/LIC pET-37b(+) pTrcHis2 C pTrcHis2 B pTrcHis2 A pET303/CT-His pET302/NT-His pRSET-CFP pRSET-EmGFP pRSET-BFP pGFPuv pET300/NT-DEST pET301/CT-DEST pGEM-T pBad43 pGEX-4T-3 pGEX-5X-2 pBlueScript SK(+) pG-Tf2 pG-KJE8 pGro7 pET-SUMO pSE380 pET-17b pET102/D-TOPO pCDFDuet
26、-1 pMAL-p5x pTf16 pET-28c(+) pBluescript II SK(+) pET-30b(+) pSUMO pProEX HTc pProEX HTb pProEX HTa pKD3 pKD13 pKD46 pTYB1 pTYB2 pTWIN2 pBluescript II KS(-) pTYB12 pMAL-p5e pACYCDuet-1 pEGM-11ZF(+) pEGM-7ZF(+) PinPoint Xa-3 PinPoint Xa-2 PinPoint Xa-1 pSP73 pSP64 pTWIN1 pTYB11 pTXB1 pET-5b(+) pBad/g
27、III C pBad/gIII B pBad/gIII A pET-5a(+) pMal-p4X pMal-p2G pkk223-3 pkk232-8 pCYB1 pEZZ18 pBAD18 pMAL-c5x pMal-p2E pMal-p2X pET-44 EK/LIC pET-43.1 EK/LIC pET-41 EK/LIC pMal-c4X pTrcHis B pET-31b(+) pET-3b(+) pET-41a(+) pGEX-3X pGEX-4T-2 pETDuet-1 pGEX-4T-1 pTrc99a pET-28b(+) pET-His pALEX a,b,c pACYC
28、177 pBR322 pKD4 pKD20 pMXB10 pEcoli-6xHN-GFPuv pKJE7 pRSET B pGEX-KG pGEX-2T pRSFDuet-1 pCOLADuet-1 pTrcHis C pTrcHis A pET-41b(+) pET-42b(+) pET-3a(+) pGEX-6P-3 pGEX-6P-2 pGEX-6P-1 pGEX-5X-3 pGEX-5X-1 pGEX-2TK pRSET A pMal-c2G pMal-c2E pMal-c2X pRSET C pQE-30 pET-45b(+) pET-44b(+) pET-42c(+) pET-41c(+) pET-40b(+) pET-33b(+) pET-39b(+) pET-32 EK/LIC pET-32 Xa/LIC pET-32c(+) pET-32b(+) pET-30 Xa/LIC pET-30 EK/LIC pET-30c(+) pET-29c(+) pET-29b(+) pET-29a(+) pET-24c(+) pET-24b(+) pET-24(+) pET-23d(+) pET-11d(+) pBad33 (
