1、Integrative analyses reveal a long noncodingRNA-mediated sponge regulatory networkin prostate cancer,NATURE COMMUNICATIONS15 MAR 2016WCY,long noncoding RNAs (lncRNAs) can function as microRNA sponges and compete for microRNA binding to protein-coding transcripts,实验目的：期望本实验识别出关键 lncRNA，揭示卵巢癌EMT中lncRN
2、A的调控机制。,we systematically identify a lncRNA-mediated sponge regulatory network of protein-coding driver genes in prostate cancer.We also validate the tumour-suppressive function of two lncRNAs predicted to serve as miRNA sponges and positively regulate PTEN expression.,Prediction of sponge lncRNAs r
3、egulating cancer-driver genes,This network contains in total 96 predicted regulatory interactions between 52 sp-lncRNAs and 17 PCGs,The regulation of PTEN expression is 3UTR-dependent,lnc-2,lnc-6,lnc-7,luciferase-PTEN-3UTR reporter system,activity is reduced,little effect,the regulation of PTEN bysp
4、-lncRNAs is through the PTEN 3UTR,siRNA-mediated konckdown,The regulation of PTEN expression is dependent on miRNAs,lnc-2,lnc-6,lnc-7,siRNA-mediated konckdown,wild-type DICER,mutant DICER,The regulation of PTEN expression is dependent on miRNAs,The determinants of sponge lncRNA efficacy,The miRNA-induced repression occurs dominantly in the cytoplasm and is mediated by RISC.qRT-PCRRIP-ChIP,Sponge lncRNAs of PTEN exert a tumour-suppressive function,cell proliferation the activity of the oncogenic PI3/Akt pathway,
