1、Genetic transformation of wheat mediated by Agrobacterium tumefaciens作者: Cheng M, Fry JE, Pang SZ, Zhou HP, Hironaka CM, Duncan DR, Conner TW, Wan YC 来源出版物: PLANT PHYSIOLOGY 卷: 115 期: 3 页: 971-980 出版年: NOV 1997 被引频次: 230 参考文献: 36 引证关系图 摘要: A rapid Agrobacterium tumefaciens-mediated transformation sy
2、stem for wheat was developed using freshly isolated immature embryos, precultured immature embryos, and embryogenic calli as explants. The explants were inoculated with a disarmed A. tumefaciens strain C58 (ABI) harboring the binary vector pMON18365 containing the beta-glucuronidase gene with an int
3、ron, and a selectable marker, the neomycin phosphotransferase II gene. Various factors were found to influence the transfer-DNA delivery efficiency, such as explant tissue and surfactants present in the inoculation medium. The inoculated immature embryos or embryogenic calli were selected on G418-co
4、ntaining media. Transgenic plants were regenerated from all three types of explants. The total time required from inoculation to the establishment of plants in soil was 2.5 to 3 months. So far, more than 100 transgenic events have been produced. Almost all transformants were morphologically normal.
5、Stable integration, expression, and inheritance of the transgenes were confirmed by molecular and genetic analysis. One to five copies of the transgene were integrated into the wheat genome without rearrangement. Approximately 35% of the transgenic plants received a single copy of the transgenes bas
6、ed on Southern analysis of 26 events. Transgenes in T-1 progeny segregated in a Mendelian fashion in most of the transgenic plants.文献类型: Article 语言: English KeyWords Plus: FERTILE TRANSGENIC WHEAT; ORYZA-SATIVA-L; TRITICUM-AESTIVUM L; FOREIGN GENES; INDICA RICE; T-DNA; MICROPROJECTILE BOMBARDMENT; S
7、ELECTABLE MARKER; RAPID PRODUCTION; L PLANTS 通讯作者地址: Cheng, M (通讯作者), MONSANTO CO, 700 CHESTERFIELD PKWY N, MAIL ZONE GG4H, ST LOUIS, MO 63198 USA 出版商: AMER SOC PLANT PHYSIOLOGISTS, 15501 MONONA DRIVE, ROCKVILLE, MD 20855 学科类别: Plant Sciences IDS 号: YF800 ISSN: 0032-0889 Transgene inheritance in pla
8、nts genetically engineered by microprojectile bombardment作者: Pawlowski WP, Somers DA 来源出版物: MOLECULAR BIOTECHNOLOGY 卷: 6 期: 1 页: 17-30 出版年: AUG 1996 被引频次: 62 参考文献: 86 引证关系图 摘要: Microprojectile bombardment to deliver DNA into plant cells represents a major breakthrough in the development of plant tra
9、nsformation technologies and accordingly has resulted in transformation of numerous species considered recalcitrant to Agrobacterium- or protoplast-mediated transformation methods. This article attempts to review the current understanding of the molecular and genetic behavior of transgenes introduce
10、d by microprojectile bombardment. The characteristic features of the transgene integration pattern resulting from DNA delivery via microprojectile bombardment include integration of the full length transgene as well as rearranged copies of the introduced DNA. Copy number of both the transgene and re
11、arranged fragments is often highly variable. Most frequently the multiple transgene copies and rearranged fragments are inherited as a single locus. However, a variable proportion of transgenic events produced by microprojectile bombardment exhibit Mendelian ratios for monogenic and digenic segregat
12、ion Vs events exhibiting segregation distortion, The potential mechanisms underlying these observations are discussed.文献类型: Review 语言: English 作者关键词: plants; stable transformation; direct DNA delivery; microprojectile bombardment; transgene expression; inheritance KeyWords Plus: DIRECT GENE-TRANSFER
13、; AGROBACTERIUM-MEDIATED TRANSFORMATION; DISCHARGE PARTICLE-ACCELERATION; HIGH-VELOCITY MICROPROJECTILES; T-DNA; FOREIGN GENES; RICE PLANTS; PETUNIA-HYBRIDA; EXOGENOUS DNA; WHEAT PLANTS 地址: 1. UNIV MINNESOTA, DEPT AGRON Biotechnology transgene expression; Triticum aestivum KeyWords Plus: DISCHARGE P
14、ARTICLE-ACCELERATION; TRITICUM-AESTIVUM L; MICROPROJECTILE BOMBARDMENT; AGROBACTERIUM-TUMEFACIENS; BETA-GLUCURONIDASE; RAPID PRODUCTION; L PLANTS; T-DNA; TRANSFORMATION; MARKER 通讯作者地址: Stoger, E (通讯作者), John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk England 地址: 1. John Innes Ctr Plant Sci Re
15、s, Norwich NR4 7UH, Norfolk England 出版商: KLUWER ACADEMIC PUBL, SPUIBOULEVARD 50, PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS 学科类别: Biochemical Research Methods; Biochemistry Biotechnology wheat (Triticum aestivum L.); glyphosate selection; Roundup Ready KeyWords Plus: FERTILE TRANSGENIC WHEAT; MICROPR
16、OJECTILE BOMBARDMENT; RICE PLANTS; GENETIC-TRANSFORMATION; BETA-GLUCURONIDASE; RAPID PRODUCTION; TUMEFACIENS; PROMOTER; EXPRESSION; INDICA 通讯作者地址: Hu, T (通讯作者), Monsanto Co, 700 Chesterfield Pkwy W, St Louis, MO 63017 USA 地址: 1. Monsanto Co, St Louis, MO 63017 USA 出版商: SPRINGER-VERLAG, 175 FIFTH AVE
17、, NEW YORK, NY 10010 USA 学科类别: Plant Sciences IDS 号: 695JB ISSN: 0721-7714 DOI: 10.1007/s00299-003-0617-6 Transgenic barley expressing a fungal xylanase gene in the endosperm of the developing grains作者: Patel M, Johnson JS, Brettell RIS, Jacobsen J, Xue GP 来源出版物: MOLECULAR BREEDING 卷: 6 期: 1 页: 113-
18、123 出版年: FEB 2000 被引频次: 41 参考文献: 38 引证关系图 摘要: The feasibility of producing plant cell wall polysaccharide-hydrolysing feed enzymes in the endosperm of barley grain was investigated. The coding region of a modified xylanase gene (xynA) from the rumen fungus, Neocallimastix patriciarum, linked with an
19、 endosperm-specific promoter from cereal storage protein genes was introduced into barley by Agrobacterium-mediated transformation. Twenty-four independently transformed barley lines with the xylanase gene were produced and analysed. The fungal xylanase was produced in the developing endosperm under
20、 the control of either the rice glutelin B-1 (GluB-1) or barley B1 hordein (Hor2-4) promoter. The rice GluB-1 promoter provided an apparently higher expression level of recombinant proteins in barley grain than the barley Hor2-4 promoter in both transient and stable expression experiments. In partic
21、ular, the mean value for the fungal xylanase activity driven by the GluB-1 promoter in the mature grains of transgenic barley was more than twice that with the Hor2-4 promoter. Expression of the xylanase transgene under these endosperm-specific promoters was not observed in the leaf, stem and root t
22、issues. Accumulation of the fungal xylanase in the developing grains of transgenic barley followed the pattern of storage protein deposition. The xylanase was stably maintained in the grain during grain maturation and desiccation and post-harvest storage. These results indicate that the cereal grain
23、 expression system may provide an economic means for large scale production of feed enzymes in the future.文献类型: Article 语言: English 作者关键词: endosperm; expression; feed enzyme; seed storage protein; transgenic barley; xylanase KeyWords Plus: NEOCALLIMASTIX-PATRICIARUM; ESCHERICHIA-COLI; PARTICLE BOMBA
24、RDMENT; TOBACCO PLANTS; HORDEIN GENE; WHEAT; TRANSFORMATION; SEQUENCE; AGROBACTERIUM; (TRANS)GENES 通讯作者地址: Xue, GP (通讯作者), CSIRO, 306 Carmody Rd, St Lucia, Qld 4067 Australia 地址: 1. CSIRO, St Lucia, Qld 4067 Australia2. CSIRO, Canberra, ACT 2601 Australia 出版商: KLUWER ACADEMIC PUBL, SPUIBOULEVARD 50,
25、 PO BOX 17, 3300 AA DORDRECHT, NETHERLANDS 学科类别: Agronomy; Plant Sciences; Genetics Horticulture IDS 号: 309GT ISSN: 1380-3743 A comparison of transgenic barley lines produced by particle bombardment and Agrobacterium-mediated techniques作者: Travella S, Ross SM, Harden J, Everett C, Snape JW, Harwood
26、WA 来源出版物: PLANT CELL REPORTS 卷: 23 期: 12 页: 780-789 出版年: MAR 2005 被引频次: 37 参考文献: 44 引证关系图 摘要: Two barley transformation systems, Agrobacterium-mediated and particle bombardment, were compared in terms of transformation efficiency, transgene copy number, expression, inheritance and physical structure
27、 of the transgenic loci using fluorescence in situ hybridisation (FISH). The efficiency of Agrobacterium-mediated transformation was double that obtained with particle bombardment. While 100% of the Agrobacterium-derived lines integrated between one and three copies of the transgene, 60% of the tran
28、sgenic lines derived by particle bombardment integrated more than eight copies of the transgene. In most of the Agrobacterium-derived lines, the integrated T-DNA was stable and inherited as a simple Mendelian trait. Transgene silencing was frequently observed in the T-1 populations of the bombardmen
29、t-derived lines. The FISH technique was able to reveal additional details of the transgene integration site. For the efficient production of transgenic barley plants, with stable transgene expression and reduced silencing, the Agrobacterium-mediated method appears to offer significant advantages ove
30、r particle bombardment.文献类型: Article 语言: English 作者关键词: barley; Agrobacterium; biolistic; transgene analysis; FISH KeyWords Plus: IN-SITU HYBRIDIZATION; HORDEUM-VULGARE; T-DNA; MICROPROJECTILE BOMBARDMENT; INSITU HYBRIDIZATION; PLANT-CELLS; TRANSFORMATION; RICE; WHEAT; GENE 通讯作者地址: Harwood, WA (通讯作者
31、 ), John Innes Ctr Plant Sci Res, Norwich Res Pk, Norwich NR4 7UH, Norfolk England 地址: 1. John Innes Ctr Plant Sci Res, Norwich NR4 7UH, Norfolk England 电子邮件地址: wendy.harwoodbbsrc.ac.uk 出版商: SPRINGER, 233 SPRING STREET, NEW YORK, NY 10013 USA 学科类别: Plant Sciences IDS 号: 905IG ISSN: 0721-7714 DOI: 10
32、.1007/s00299-004-0892-x Transgenic rice: A valuable monocot system for crop improvement and gene research作者: Tyagi AK, Mohanty A, Bajaj S, Chaudhury A, Maheshwari SC 来源出版物: CRITICAL REVIEWS IN BIOTECHNOLOGY 卷: 19 期: 1 页: 41-79 出版年: 1999 被引频次: 36 参考文献: 230 引证关系图 摘要: Since the first fertile transgenic
33、 rice was obtained during the late 1980s, studies on rice transformation have undergone rapid strides. Several physical methods of gene delivery, including Agrobacterium, have been employed to produce transgenic rice. Up to now, about 50 rice cultivars have been transformed that include many japonic
34、a and also a few indica cultivars. Consequent to the availability of an efficient transformation system in rice, the expression of monocot genes is better understood because expression of several genes and regulatory elements from rice and other related cereals such as wheat has been studied in rice
35、. Genes of agronomic importance for herbicide, insect, virus, and fungal resistance have been introduced in rice, and some of the transgenics have already completed a few years of field trials. In this context, rice is being looked on as a model monocot plant to study gene expression and to introduc
36、e agronomically useful genes. While this progress is expected to supplement conventional breeding program of rice improvement in the near future, some impediments in the successful implementation of transgene technology, such as gene silencing, the presence of undesirable selection/marker genes, hig
37、h cost, and constitutive vs. targeted/optimal expression of genes, are addressed.文献类型: Review 语言: English 作者关键词: transgenic rice; Oryza sativa; gene expression; crop improvement KeyWords Plus: ORYZA-SATIVA-L; DISCHARGE PARTICLE-ACCELERATION; AGROBACTERIUM-MEDIATED TRANSFORMATION; TUNGRO BACILLIFORM
38、VIRUS; BETA-GLUCURONIDASE GENE; HYGROMYCIN RESISTANCE GENE; TISSUE-SPECIFIC EXPRESSION; CAUSES ALTERED MORPHOLOGY; MAIZE UBIQUITIN PROMOTER; SUSPENSION-CULTURE CELLS 通讯作者地址: Tyagi, AK (通讯作者), Univ Delhi, Ctr Plant Mol Biol, S Campus,Benito Juarez Rd, New Delhi 110021, India 地址: 1. Univ Delhi, Ctr Pl
39、ant Mol Biol, New Delhi 110021, India2. Univ Delhi, Dept Plant Mol Biol, New Delhi 110021, India 出版商: CRC PRESS INC, 2000 CORPORATE BLVD NW, JOURNALS CUSTOMER SERVICE, BOCA RATON, FL 33431 USA 学科类别: Biotechnology DNA methylation; DDM1; Arabidopsis suecica; allopolyploid KeyWords Plus: ARABIDOPSIS-TH
40、ALIANA; NUCLEOLAR DOMINANCE; DNA METHYLATION; GENOME EVOLUTION; PLANTS; WHEAT; ALLOTETRAPLOIDS; ALLOPOLYPLOIDY; ELIMINATION; ANGIOSPERMS 通讯作者地址: Pikaard, CS (通讯作者), Washington Univ, Dept Biol, 1 Brookings Dr, St Louis, MO 63130 USA 地址: 1. Washington Univ, Dept Biol, St Louis, MO 63130 USA 电子邮件地址: pi
41、kaardbiology.wustl.edu 出版商: BLACKWELL PUBLISHING LTD, 9600 GARSINGTON RD, OXFORD OX4 2DG, OXON, ENGLAND 学科类别: Plant Sciences IDS 号: 721ZW ISSN: 0960-7412 DOI: 10.1046/j.1365-313X.2003.01857.x Genetic enrichment of cereal crops via alien gene transfer: New challenges作者: Repellin A, Baga M, Jauhar PP,
42、 Chibbar RN 来源出版物: PLANT CELL TISSUE AND ORGAN CULTURE 卷 : 64 期: 2-3 页: 159-183 出版年: 2001 被引频次: 32 参考文献: 243 引证关系图 摘要: Genetic improvement of crops has traditionally been achieved through sexual hybridization between related species, which has resulted in numerous cultivars with high yields and supe
43、rior agronomic performance. Conventional plant breeding, sometimes combined with classical cytogenetic techniques, continues to be the main method of cereal crop improvement. More recently, through the introduction of new tools of biotechnology, crossing barriers have been overcome, and genes from u
44、nrelated sources have become available to be introduced asexually into plants. Cereal crops were initially difficult to genetically engineer, mainly due to their recalcitrance to in vitro regeneration and their resistance to Agrobacterium infection. Systematic screening of cultivars and explant tiss
45、ues for regeneration potential, development of various DNA delivery methods and optimization of gene expression cassettes have produced transformation protocols for the major cereals, although some elite cultivars still remain recalcitrant to transformation. Most of the transgenic cereals developed
46、for commercial purpose exhibit herbicide and/or insect resistance; traits that are often controlled by a single gene. In recent years, more complex traits, such as dough functionality in wheat and nutritional quality of rice have been improved by the use of biotechnology. The current challenges for
47、genetic engineering of plants will be to understand and control factors causing transgene silencing, instability and rearrangement, which are often seen in transgenic plants and highly undesirable in lines to be used for crop development. Further improvement of current cereal cultivars is expected t
48、o benefit greatly from information emerging from the areas of genomics, proteomics and bioinformatics.文献类型: Review 语言: English 作者关键词: alien gene transfer; barley; fluorescent in situ hybridization; genetic engineering; homoeologous chromosome pairing; in vitro regeneration; maize; oat; pearl millet;
49、 sorghum; transformation; wheat; wide hybridization KeyWords Plus: TRANSGENIC RICE PLANTS; YELLOW STEM BORER; ORYZA-SATIVA L; GREEN FLUORESCENT PROTEIN; STILBENE SYNTHASE GENE; DISCHARGE PARTICLE-ACCELERATION; WHISKER-MEDIATED TRANSFORMATION; ARTIFICIAL CHROMOSOME LIBRARY; MATRIX ATTACHMENT REGIONS; SYNTHETIC CRYIA(B) GENE 通讯作者地址: Chibbar, RN (通讯作者), Natl Res Council Canada, Inst Plant Biotechnol, 110 Gymnasium Pl, Saskatoon, SK S7N 0W9 Canada 地址: 1. Natl Res Council Canada, Inst Plant Biotechnol, Saskatoon, SK S7N 0W9 Canada2
