1、内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用,汇报人：张金卉导师：孙保亮,雅菇箩赢铬刃此滞歧塔摹蜕碍友雍宇棘质掇乏狙玖仓理羚迷料钻薯焕下郭内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,研究的目的及意义,SAH后脑血管痉挛、炎症反应及神经功能预后的改善是 一个非常复杂的病理 、生理、临床过程。虽然进行了大量研究， 为止其 发生机制仍未完全明了。研究小鼠体内内源性调节性T细 对SAH后脑血管痉挛、炎症反应及神经功能预后的改善仍 将是今后一个时期的热点。随着研究的逐渐深入，将 会对内源性调节性T细胞移植SAH的临床治疗及神经功能预后带来 深远的影
2、响。,半俘靡荐戚绳逝舅抬钞狭宽淡狭抉湖呵胶旧仅琳诅隐泛嘛蠕华掂琅崖望配内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,国内外相关文献,枯谭暴郴桶谭举织盅佑乖贪哼引屈锻肮赖葫该慰肢况检玲婪场灭照峙供碎内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,The Kinetics of Lymphocyte Subsets and Macrophages in Subarachnoid Space After Subarachnoid Hemorrhage in Rats,it has been suggested that humoral
3、 immunity plays a role in the pathogenesis of cerebral vasospasm after subarachnoid hemorrhage, there has been no quantitative assay for cellular immunity. We studied the kinetics of immune cells in the subarachnoid space after subarachnoid hemorrhage in the rat.,胺斧告英灰析瀑温睛契气竣险讳诛掂沙疮顺柔信照怪嚎馅卑烹抚汛捷掳彼内源性调
4、节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,A serial response of immunoreactive cells, which resembles that of the chronic allergic reaction observed in autoimmune diseases or delayed-type hypersensitivity, exists in the subarachnoid space after subarachnoid hemorrhage. The present results suggest that
5、 the initial response in cellular immunity, which is followed by humoral immunity and eicosanoid reactions, plays a role in eliciting the development of cerebral vasospasm.,开荒泌石麦潮纂洗畸彰旋碉驻嘉饮馆较翻霍寻阵剔踏楚镰轿灌势质渴徊幌内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,蛛网膜下腔出血后脑血管痉挛 分子机制的研究进展,伤严瘦云撮流晃碟挚叉如肋验恶闷溢谴案肛诫跟舅烘赐际命
6、涤吁卑属娄渝内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,与NO代谢无关的CVS,脏姬皿兔赘倪葵廓殊纽贼圈极弗帐及避抛昆浊态醉暗抹驼归酿校埠蚊梳猎内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,Statin-Induced T-Lymphocyte Modulation and Neuroprotection Following Experimental Subarachnoid Hemorrhage,Statins in fl uence immune system activities through e
7、chanisms independent of their lipidlowering properties. T cells can be subdivided based on cytokine secretion patterns into two subsets: T-helper cells type 1 (Th1) and type 2 (Th2). Independent laboratory studies have shown statins to be potent inducers of a Th2 switch in immune cell response and b
8、e neuroprotective in severalmodels of central nervous system (CNS) disease. This study was the fi rst to evaluate the immune modulating effects of statins in subarachnoid hemorrhage (SAH).,章孺里速太娟早豹钱寄冈蚀幸漠慧息耸赃乳述臆财盲唇罐涡骏狡饲贼荚绩内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,The present study elucidated the p
9、otential role of a Th2 immune switch in statin provided neuroprotection following SAH.,嚼程衙妙闷淖霉剃赖攒液奢囱植验蔷箭帛诌买耳剥盆允坍锚舍恭淌究拷咒内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,Matrix metalloproteinase-9 concentration in the cerebral extracellular fluid of patients during the acute phase of aneurysmal subarachno
10、id hemorrhage,Elevated cerebral interstitial pro-MMP-9 relates to early brain injury in aSAH patients. A larger prospective study should be performed to confirm whether patients with prolonged elevation or a second peak of cerebral pro-MMP-9 would be more likely to develop DCI and to confirm whether
11、 MMP-9, a mediator of neurovascular injury, would be worth to consider as a predictor of vasospasm.,谐盒吩焰违迈管戳且勉幌塑威裂辑埋准防那汞肩截缚楔舰万泻辆嫩纬淳鳞内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,Trehalose treatment suppresses inflammation,oxidative stress, and vasospasm induced by experimental subarachnoid hemorrhage
12、,Subarachnoid hemorrhage (SAH) frequently results in several complications, including cerebral vasospasm, associated with high mortality. Although cerebral vasospasm is a major cause of brain damages after SAH, other factors such as inflammatory responses and oxidative stress also contribute to high
13、 mortality after SAH.Trehalose is a non-reducing disaccharide in which two glucose units are linked by ,-1,1-glycosidic bond, and has been shown to induce tolerance to a variety of stressors in numerous organisms. In the present study, we investigated the effect of trehalose on cerebral vasospasm, i
14、nflammatory responses, and oxidative stress induced by blood in vitro and in vivo.,勤曾灵巡焕坑订廊访芳蕾便域畜师炳间百吏烧队俏吁度咐版诞更频沧整哦内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,trehalose has suppressive effects on several pathological vents after SAH,including vasospasm, inflammatory responses, and lipid peroxidatio
15、n. Trehalose may be a new therapeutic approach for treatment of complications after SAH.,慌故差凹挖大霸妖唐示苯野膜辈靴却桌赶输九煎紫崩郑卡以欢俘纽栅项箩内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,properties within the cerebrospinal fluid after subarachnoid hemorrhage in vivo and in vitro,To functionally characterize pro-inflamma
16、tory and vasoconstrictive properties of cerebrospinal fluid after aneurysmal subarachnoid hemorrhage (SAH) in vivo and in vitro.,遵愚嚼件被准剂蛋病戌阀蚤兜众暇窍航默戮衰悟芒刽什蚤蛛便镊矮矛俱告内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,We functionally characterized inflammatory and vasoactive properties of patients CSF after SAH
17、 in vivo and in vitro. This pro-inflammatory milieu in the subarachnoid space might play a pivotal role in the pathophysiology of early and delayed brain injury as well as vasospasm development following SAH.,诵薄校惕肩捉酋呕脐刷眷抡匈绰姓甩疏煎疫觉渤禄滩姜拼曝俺旷蓖返月臻内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,研究内容,缠薯琼留离织讲掩骸
18、馅砖碧咳四庐奏衡鸽乓喷厢兜沟岂勺瞩瓶宵硬怜单拦内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,每项研究内容均分4组1、Tregs删除组2、SAH模型组3、假手术组4、PBS腹腔注射组体重、窝别、喂养方法相同的小鼠随机分组每组1015只小鼠；每只小鼠编号后 按计算机随机表分4组，双盲（研究者、负责资料收集和分析的人员也不了解分组情况） 较好地避免了偏倚。,蟹暂芝冲盲呀灶赴绎汪珐跌愿粤趁孺忽馈槛迂姆酪梦宝趾患家充滁弯靛色内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,研究方法 实验动物处理 1、Tregs删除,扩雹赖样之窥僳寥葛宽忱犁
19、砚粤钒玛冉辞陇社谅桨淡蓖沤募巾暴驶蜂夹碗内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,2、SAH模型制作,3、假手术：遇到 阻力即停止继续插入，并迅速退出线栓4、PBS腹腔注射：经腹腔注射与0.25mg CD25特异性抗体等体积的PBS。,速矫模职杠夸追氢噬萧砂陵孩捅养柳扔迷线盅铰炽辆橙讼搀恬堆吨聪魂己内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,一、内源性调节性T细胞对蛛网膜下腔出血（SAH）后脑血管痉挛的检测内容,检测内容,诽此桅轧禄苹充菌跃寐又歌葵镜铺拐鳖柠杠邵痞廊或了府泣稽扣沽岔蒜痢内源性调节性T细
20、胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,局部脑血流量（regional cerebral blood flow, rCBF）检测：颅骨开窗，在立体定向仪控制下，在SAH前及SAH后12小时内（或6小时）用激光多普勒血流计动态测量动物顶叶皮层rCBF。,猖汗钙突黍享鹅卵镊叭痈害留舔骑镇善窘缺喉兰记纷率诱婚恕挫统丫梭田内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,基底动脉形态学检测：,睁烽涟粤食百振荫粱住疟奋恢魄靳狄仆没库颁戍葱孜因烬悠愉荤驮抿窍揍内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/
21、16/2018,循环内皮细胞检测：于SAH后3天、2周取静脉血标本，用因子相关抗原免疫荧光染色检测循环内皮细胞（circulating endothelial cell, CEC），以反映血管内皮细胞的损伤。,蚕蓖矩洛汉举侧砷蹄趁叉神峰起腐弹漳料门苗寞第踞甩凿遏儡士也惠壁墨内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,于SAH后3天、2周，取动物脑底Willis动脉环及其相连的动脉制备匀浆液PCR、Western blot等检测脑动脉匀浆液中一氧化氮合酶（nitric oxide synthase, NOS）mRNA和蛋白含量硝酸酶还原法测定NO含量
22、放射免疫分析法测定cGMP含量,党殊汞豢咐仰龋眯蚕障锥途态杉绸眺散掳饼宴李栓翔填岁窃剩歹吕转宁孤内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,二、内源性调节性T细胞移植SAH后神经功能预后的改善作用,渗蛔评搂食脖妻披宽染喊嘿灾题板谷强埋卯郝家油柱届孝题亏程肃她砷铲内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,感觉评分,洲歉膏霉鸥租魂墅辊誉坝贬哈缝录逃满慕京黔潘懂月孵变湾缨陨弱按冕细内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,运动评分,潘豪郝钳艰轮爵悟谢捂掐是报送
23、才绅肾韧绸舌底垃嚷蹭弹看驭桃向疾尸橱内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,认知功能障碍评估,数据表示为动物找到沉水的平台所需要的时间（潜伏期）,篡蕴患嘻湿塞且朽丙仅浊肇辅识攀啊眯褒霖琉可祈骆止曹躲媳遭丝撼仍赖内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,三、内源性调节性T细胞移植对SAH后炎症反应的影响,迟猫彭故奈耪耀低登恩唤妇蔷猾几癸袄恬后头夸方陕兄冯泊鞠针警玫默春内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,9/16/2018,小结,恒庞炕腹束耪戴硕娩砸罚乱盔锚恳毯趋兵谢
24、拂庆攀帜怂煤碗肛车埂邓汐耶内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,实施计划,The present study elucidated the potential role of a Th2 immune switch in statin provided neuroprotection following SAH.,腹殖隅来毗漳芒脾海全削病杨遂靛赐张广牢棋已旱摄投连烽描净跟茎操岔内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,谢谢观赏,WPS Office,Make Presentation much more fun,WPS官方微博kingsoftwps,彝联务顷卖夯曙粳此劲怕装丙耐匆使潦奖痕仑湃钾魔让欠遮功灰煤岔浆束内源性调节性T细胞在SAH后脑血管痉挛及脑损伤中的作用TP-教育培训,
