1、可注射聚丙交酯多孔微载体制备及与骨髓基质干细胞共培养研究2006?8?(?s2?s4?b?8?R?T?,?9?S?l?m?j?)?z?189?WM?W3?l?+?f?w?,?9?R?(PDLLA)?T?W0?T?z?Q?C?c?S?6?A?m?,?9?W0?SEM?1?Z?X?T?P?W2?T?A?l3351xm-+651xm?6?A?20txm?A?L?80?4172?DW0?+?Z?X?l?l?P?V?b?W0?6?A?P?T?L?P?y?V?b?A?T?8?E?1?W?X?)?bW0WM?B?W3?R?T?+?f?w?l?8WM?C?1?W3R318?S08WM?B?9?2W3AWM?+
2、?W31673rm0364(2006)?04rm0189rm04TheFabricationofInjectablePolylactidePorousMicrocarriersfortheCultureoftheBoneMarrowStromaCellsWANGTun?ADAIGuanghui4?AQ?Daping,LIA0Kairong,JzANGXiaodan4?AXURuxian94?SSchoolofChemistryandChemicalEngineering,SunYat-senUniversity,Guangzhou510275?AChina?h4NeuromedicalInst
3、itute?AZhujiangHospitalofSouthernMedicalUniversity,Guangzhou510282?AChina?SWMAbstractW3ObjecfiveTodevelopamicrocarrierwhichtheporessizeandtheporosityofthecouldbecontrolledbychangingtheconcentrationoftheammoniumbicarbonatesolution?Aandinvestigatetheeffectsofpreparationconditiononthesizeandmorphologyo
4、fthemicroearrier?SMethodsAninjectablepolyrmD?ALrmlacticacidPorousscaffoldmicrocarrierwasfabricatedbythemethodofmodifiedwaterrminrmoilrminrmwater(W?A?E0?E?2)?SThemicrocarrierswerecharacterizedbymeansofSEMandgranularityanalysis?SResultsSEMshowedthattheaveragediameterofthemicrosphereswas3351xm?651_Lman
5、dthediameteroftheporeswaslargerthan20pW0mthatwaslargeenoughforcellinfiltrationandseeding?STheimagesofSEMtestifiedthatthemieroearrierspreparedusinggreateramountofammoniumbicarbonatehadlargerinternalporevolume?STheresuhsofcellculturedshowedthatBMSCgrownwellonthesurfaceandininner?SConclusionTheseporous
6、scaffoldmicroearrierscouldbeusedfordeliveringtheseededcellstothetissuedefectsiteinaninjectablemanner?SWMKeywordsW3Biodegradablepolymer?hPorousMicrocarrier?hDoubleemulsion?hPolylaeticacid?hBoneMarrowStromaCells?hInjectable?L?w?T?J?l?+?,?M?w?WMlW3?A?T?8?M?L?w?W?u?W0?8?M?w?N?T?h?w?)?A?l?3?n?)W2?W2?)?R?
7、X?)?P?O?J?l?YW0?8?L?w?j?T?l?Q?G?S?A?T?L?w?l?T?,?(30270393)?h?C?j?+?S?+?s?h?+?(200629)W0?4?T510275?e?2?0?C?j?+?S?+?L?1?m?O(?A?j?)?A?)?h510282?e?2?0?U?j?U?U?m?O(?A?z?A?)W0?T?4?T?j?)?r?8?L?w?W0?T?J?P?n?E?T?6451?4?WMq?A?4?V?l?6?A?S?4?1?4?l?6?A?l?U?w?V?P?S?A?4?)?y?_?b?W0?A?,?9?W2?P?T?L?6?N?A?1?P?y?V?T?A?
8、L?P?y?f?l?r?b?W0Kima?c?X?,?T?+?w?,?9PLGA?6?A?T?1?w?W?P?T?171W0KWChun?JournalofTissueEngineeringandReconstructiveSurgery?AAugust?S2006V01?S2No?S4?+?+?w?,?9?PLGA?6?A?m?1?u?P?Q?jW2?I?b?p?j?I?p?j?V?PI?81W0?N?S?A?,?A?l?L?D?6?V?b?A?B?)?TW0?s?z?+?f?w?,?9?J?,?A?c?,?9?W2?L?W2?A?T?l?l?V?S?b?l?L?C?l?YW01?11?S1
9、?R?(PDLLA)?s?(?M(Mr?E=16?S1xlca?T?(PVA)?T?1?(Mw300?500)?h?c?(?1?A?y?f?j?0?+?)?hpH7?S4?x?b?1?Q(PBS)(?1?u?1?S6369NaCl?A0?S0409KCl?AO?S7169Na2HPOW0?A0?S0499KH2P04?Q?C?A?200m1)?h?X?1?W01?S2?,?9?l?+?f?w?,?9?T?12?S5ml?)?A?c?Q?15ml?X0?S339?Eml?PDLLA?w?G?CW0?+?1?1?1?A?M?F?W0?1?D?F?100ml?X1?4PVA?+?Q?A?A?1?Z?
10、300ml?X0?S1?4PVA?W0?D?W?EO?EW?l300rpm?Emin?L?f?4?E?A?f?=?1?S?S?l?Q?)?t?2?i?3?i?A?6?PVAW0?S?C?W01?S3?j?l?m?lMastersizer2000?2?1?T?l?JSM?6330F?X?8?p?Z?6?W0?u?Q?T?6?l70?4?A?l?Q?0?t3?i?A?,?S?l?x?C?12?E?A?W0?u?Q?T?L?E?TWateruptakeratio=(?Wca?E?C?Wd?1?u?w?u?Q?6?6?l?W01W04?S?l?m?N?l?(BMSCs)?l?L?l?C?P?j?XW2
11、?)?A?)?I?1?W2?7?A?6?W?l?d?BMSCs?)?)?C?(CNS)?m?C?A?S?b?(W2?1?j?j?3BMSCs?l?)?L?C?_?NW2?A?BMSCs?A?=?s?BMSCs?X?W2?V?S?b?W01?S4?S1?2209?2509?SD?j?r?A?2?L?810?4?Q?(?w?(0?S4ml?E1009?)?A?5ml?10ml?ul?4?b?1?Q?t?W2?u?t?A?A?S?j?r?8?W0?P3mlFicollrmPaque?1?(?1?S077)?A?1?u?t?x?FicollrmPaque?1?D?S?2500rpm?Eminx20mi
12、n?S?S?S?Z?1?A?N?D?L?i?X?T?W2?P?J?(?uBMSCs)W2?1?W2?W0?E?J?u?P?J?SBMSCs?4?u?C?h?i?S(1000rprodminxlomin)W2?lml?2ml?Q(4i61?z?1?1?2?1?A?t?Q?T?W2?+BMSCs?h?10IIll?u10?4?O?4?DMEM?EF12?tW0800rpm?Emin?5?1?W0?D?4?A?uBMSCs?A?A?W01?S412?r?BMSCs?DMEM?EF12?A?6?Wf1?2)x105?d?E?S?A?24?L?A?1?S5ml?A?O?4(20?4?)?A?l37i6W
13、25?4C0?TW2?L?W072?E?S?V?A?S?3?y?4?y?1?iW0?1?2?S?Q?s?A?r?W01?S4?S3?8?1?SBMSCs?100mg?75?4?E?S?A?l?t?iW0?1?6?C?SBMSCs?A?27?y?S?A?CK2?(OLYMPAS?AEt?s)?5?Z?L?b?W0?6?1?S?l2?4?D?e?A?lPBS?t?6?i?A?K?l?B?S?W0?p?V?W02?S?2?S1?PDLLA?T?L?r?1?C?(?W2?(?1?W2?P?X?2006?8?(?s2?s4?b?Q?+?PVA?P?S?Z?W0?C?l?1?PDLLA?O?,?9?6?S
14、?Z?1?PDLLA?O?,?9?6?A?S?(?1?Q?j?X?S?l?Q?C?1?j?P?W0?l300?m?P?y?V?b?W0?A?T?8?E?)?A?=?s?P?1?X16?S1xlOW0?PDLLA?X?W0?l?+?w?,?9?6?A?Q?C?d?uPVA?+?S?N?c?Q?x?1?A?b?m?W?+?l?S?A?+?1?w?E0?g?B?rW0?1?F?b?Z?j?+?Q?C?A?M?E0?EW?+?A?O?P?X?f?W0?6?+?A?A?Q?C?c?1?S?M?6?W?T?(?1)W0?Z?,?A?l?P?X?f?L?C?A?y?4?b?6?W2?6?L?j?E?T?b?
15、W0?C?A?4?b?E?A?6?jW0?X?,?9?l3001?Szm?6?A?4?b?P?600rpm?EminW0?A?6?,?9?X?TPDLLA?=16?S1x104?hCPDLLA=O?S339?EmL?hVo?TVw=6?T1?h?4?b?=600rpm?EminW02?S2?PDLLA?T?L?L?r?1?6?M?L?a?X?1?LW0?Z?,?Q?C?c?1?b?m?T?A?M?L?a?N?T(?1)W0?l?A?L?6?J?Q?l?L?D?r?6?C?L?j?E?A?2?1?S?Q?C?Q?C?c?u?W0?6?J?Q?j?A?r?6?j?A?c?u?j?W10?4?D?W
16、0?6?J?Q?S?Z?j?A?S?6?a?S?Z?T(?3)W0?Y?E?w?Q?C?)?u?c?6?T?s?W?P?L?6?A?k?3?L?d?c?6?L?a?X?E?51xmW0?c?S?A?X?j?W201,zm?50xrn?A?l?V?w?P?y?W0?X?T?z?Q?C?c?u?6?L?A?u?j?10?4?A?L?S?0?(?V?b?W0?SEM?(?3)?L?A?6?l2001,zm?3001xm?A?S?1?E?A?U?w?N?L?-?6?P?,?y?A?k?X?6?6?0?(?T?8?)?W0?6?L?l10pm?30?7m?A?E?h?6?a?T?A?U?1?6?l20p
17、mw50pW0m?S?B?j?201,zm?L?0?(?1?+?_?A?A?E?P?y?f?l?W0Fig1PorousmicrospheresfabricatedbyW?EO?EWdoubleemulsionNH2HC03concentration(wt?4)Fig2ChangesofwateruptakeratioofporousmicrosphereswiththeconcentrationofammoniumbicarbonatesolutionTable1Theeffectsofconcentrationofammoniumbicarbonatesolutiononthesizeof
18、microspheresandthewateruptakeratioofthemicrospheressamplesTheconcentrationofMeanparticlePolydispersitywateruptakeammoniumbicarbonatesize(p?o(?4)solution(?4)2W03BMSCs?lPDLLA?D?V?S?b?Z?(?4)?Z?X?6?l?C?A?W?V?l?6?W0?6?L?A?V?A?+?4?A?V?l?6?S?ZW0?p?(?5)?Z?X?6?)?S?+?S?Z(?S?3-h?)?A?s?N?(?5-aW2b?A?Q?O?X)?A?6?(?5-c)?A?S?lPDLLA?D?P?jW0?T?L?P?y?V?b?W0
