1、纪念安徽针灸经络研究50周年,穴位肥大细胞及其颗粒物在针刺镇痛中的作用,针灸经络研究回顾与展望国际学术研讨会,2010.10.16,Outline,穴位肥大细胞在针刺镇痛效应中的作用穴位胶原纤维在针刺镇痛效应中的作用穴位注射组胺的镇痛效应手针和电针穴位信号的启动机制,Mast cells in acupoints and sideward,THE JOURNAL OF SCIENCE & HEALING, 2008，4（3），170-177,1. The relationship betweenmast cell function and acupuncture effect,Animal m
2、odel ：AA (acute arthritis) Rat,Complete Freunds adjuvant,Acupoint selected： Zushanli (ST36),D,1. The relationship betweenmast cell function and acupuncture effect,Treat effect：Ease pain，Pain threshold,1. The relationship betweenmast cell function and acupuncture effect,The degranulation of mast cell
3、s,SD RatsTail Flick ModelAnatomy Position of ZusanliAcupunctureInjection drugs for prevent degranulation of MC,A,B,D,E,Pain threshold of rat when shield mast cell degranulation,1. The relationship betweenmast cell function and acupuncture effect,各组别大鼠痛阈变化率峰值比较（20分钟时）(n=6),Note: *P0.05 vs. Group A; P
4、0.01, P0.05 vs. Group B1; P0.01, P0.05 vs. Group D1,1. The relationship betweenmast cell function and acupuncture effect,穴区组织肥大细胞脱颗粒率统计 (n=6),Note: *P0.01, *P0.05 vs. Control Group; P0.01 vs. Acupuncture Group.,Results from Experiment,Staining of Mast Cells in Acupoint on Rats,Muscle,Skin,Before Acu
5、puncture,After AcupunctureDegranulationof cells,After SDCG Stabilized,THE JOURNAL OF SCIENCE & HEALING, 2008，4（3），170-177,What is the key factor to degranulation,The acupuncture effect is positive relationship to the degranulation of mast cell at acupoint.What is key factor conduce the degranulation
6、 of mast cell during acupuncture manipulation ?Our focus is on the collagen at acupoint.,2018/7/15,13,To observe the function of collagen fibers in MA analgesia.7 Animal groups setting:,normal group (N): Normal healthy animal control group (M):Animal with AA injection of collagenase at Zusanli(ST36)
7、 group (C) manual acupuncture group with rotation manipulation (R) or lift-thrust manipulation (L), rotation group with collagenase pretreatment to destroy Zusanli(ST36) collagen fibers (CR) , lift-thrust group with collagenase pretreatment to destroy Zusanli(ST36) collagen fibers (CL) , lift-thrust
8、 group pretreated with collagenase to destroy Dubi(ST35) collagen fibers (CDL),(R),(L),2018/7/15,14,Analgesia effects observed in different groups,注：较正常组痛阈：* P0.01；较处理前痛阈： P0.05；较捻转组(R)痛阈变化率： P0.05； 较捻转组(L)痛阈变化率： P0.05； 较正常组或模型组: P0.05；较捻转组或提插组: P0.05。,2018/7/15,15,Analgesia effects observed in diff
9、erent groups,注：较正常组痛阈：* P0.01；较处理前痛阈： P0.05；较捻转组(R)痛阈变化率： P0.05； 较捻转组(L)痛阈变化率： P0.05； 较正常组或模型组: P0.05；较捻转组或提插组: P0.05。,2018/7/15,16,Analgesia effects observed in different groups,注：较正常组痛阈：* P0.01；较处理前痛阈： P0.05；较捻转组(R)痛阈变化率： P0.05； 较捻转组(L)痛阈变化率： P0.05； 较正常组或模型组: P0.05；较捻转组或提插组: P0.05。,collagen fibers
10、 of skin and muscle,Mast cells in skin,GroupNormal,GroupInjectControl,collagen fibers of skin and muscle,Mast cells in skin,Connective Tissue Research,2008，50（2）: 110-120,Acupunc.whirling,Acupunc.lift and thrust,collagen fibers of skin and muscle,Mast cells in skin,collagen fibers of skin and muscle
11、,Mast cells in skin,Connective Tissue Research,2008，50（2）: 110-120,Mast cells degranulation before and after collagen destroyed,Collagen destroyed,Collagen destroyed,Connective Tissue Research,2008，50（2）: 110-120,collagen fibers and mast cells in normal acupoint,collagen fibers enlacement and mast c
12、ells degranulation while whirling,collagen fibers tightened and mast cells degranulated while lifting and thrusting,collagen fibers were destroyed and mast cells had no degranulation,Connective Tissue Research,2008，50（2）: 110-120,2018/7/15,21,The mast cells in acupoint may translate the mechanical s
13、ignal into the biological information of neural system .,The collagen and mast cells degranulation are very important to the manual acupuncture analgesia effect.,Is this the same as electric acupuncture?,2018/7/15,22,手针及电针治疗前后AA大鼠痛阈和痛阈改变率以及穴区肥大细胞脱颗粒率的变化,注：与 正常组相比较：* P0.05；与 模型组相比较： P0.05； 与手针组相比较： P
14、0.05； P0.01；与电针组相比较： P0.05。,3. 穴位皮下注射组胺的镇痛疗效,各组痛阈测量比较,3. 穴位皮下注射组胺的镇痛疗效,各组痛阈测量比较,针刺研究，2010，35（2）：99-103,3. 穴位皮下注射组胺的镇痛疗效,各组痛阈测量比较,针刺研究，2010，35（2）：99-103,3. 组胺H1受体在传递针刺信号中的作用,各组足三里穴位处脱颗粒比较,P0.01 与空白组比较（vs. control）；P0.01 与模型组比较（vs. model）；P0.01 与手针针刺组比较（vs. control）；P0.01 与生理盐水穴位注射组比较；P0.01 与组胺穴位注射组比较
15、，P0.01 与组胺H1拮抗剂穴位注射组比较。,针刺研究，2010，35（2）：99-103,3. 组胺H1受体在传递针刺信号中的作用,各组足三里穴位处脱颗粒比较,P0.01 与空白组比较（vs. control）；P0.01 与模型组比较（vs. model）；P0.01 与手针针刺组比较（vs. control）；P0.01 与生理盐水穴位注射组比较；P0.01 与组胺穴位注射组比较，P0.01 与组胺H1拮抗剂穴位注射组比较。,针刺研究，2010，35（2）：99-103,穴位处组胺及H1受体,组胺参与到了针刺麻醉的穴位局部机制通过H1受体进一步传递针刺信号,研究阐明了手针和电针穴位针刺有效信号的启动机制,MA,EA,Thank you,
