1、8 F9 8 2 3 C AT APH T YO P H YSI O L O G I S AI NIC A Vo l .8 , N o . 1 F e b .f982: NP A L ,i !1I C o A +s。 ZE M l、iV150 0 . v,Fh“” ( M “ aP ) ,h: “X5|” 。|vl(、 125 15 gQIV ;hvT L 。! , 0 m mudu, M10 m m , M2 mm , ! $,V s,b r! ,F a !A(1: 1) ,2o5 C 。 .,.h)s、B !Zr( 1979)E,|19hv ,F10 mt ) ,15s,|1 mlAGQ
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11、er n t Pe ir o d in in c u ba tio n w ith t o x in . (phSF- HW) a cy e lo he x im ide wa s ad de d at 0 h r b c y c lo hXx imide w as ad d ed a t 14 hr e cy e lo hex im ide w as ad d ed at 24 h r d yC e lo hex im ide-5 a dded a t 34 hr c y c lhoJm ideBfer e co ntr o l 1:i=.0 8BL om g /m l,Fm“s , P A
12、 L?。|I lA0 . 01 M ApH 8 . 8 i24l H,5P AL?i ,TI lAFO r 0 . 6%i,7;1l H, =O ,T P AL ?(V2 ) 。 V2Ivs0 T a b le 2 . T h e p r o Pe r t ie s o f m a e r o m o lce lu a r in hibito r fr o m P e a k l 、 1: . + + ; 、I c 0 A +s- . 7h“X6|”sY9 0 . 81 . 3。Fier(1 97 3)eH n de oB n ( 19 75) T 。 He n de sr o n ( 19
13、75)fhv! c , 9?CFh1h。VnP ALBil Q Fhs。y NI nP A LMT . fhFh 3S N。 、bL) D- i H,bL) D-?r AL9,bL) D -?M P AL9T B( C19 8 1) , V OP A L 9F P AL 3 。 i-(2 0M )r AL9,7i(2M)- M! HWF HP A L Y (m3 ) ,+Y 1: , 。 , V . 70 . 60 + lm l b uffe r T + lm l in hib ito r U0004321 00 0 0 . 1 0 . 2 0 . 3 0 4 0 . 5 0 . 6 0 .
14、7 0 . 8 0 . 9 1 . 0 En z y m e (m l) g.0 kBB-。Wv V m5 PAvs0 9 . 5 T h e tyP e o f in hi bitio n . o f m a e r o m o le e u la r in hibitio n o n P A L . s0 TilC7 S i。B。 ls0w1 ?F%! P?&dSyr m gy lpor p a en,7hF%! 5 !?&dg u a ia cPor Pa ne。 L, )Hq/ Fh“” COA TK(mK = 18 . 8M ),Q 0 、4e ( K msY4 . 4, 45 .
15、1+M) ,7 !?&d -8。M1/h“X6|” Co A T(mK = 59 . 6M) “”sB。BT H en der so n ( 1975)1Fhh! c TM。 42 38 ID T Zr, 19 79。hZE。j . 、 C, 198 1。&d 1 . v&d I 4B M# Y. 3, 7: 373B38 f A tt r id g e , T . H , S mi th , H , 1973. E v id e n e e fo r a p o o l o f in a e t iv e p h eny la la n in e a mm o n iaBls e in Q“
16、 um as t。u s s e e d lin g s . C ot c he m , 12: 2569B15 74 . A s a da , Y . , M a t su m o t o , 1 . , 1972 . T he n a t u r e o f lig n in o bt a in ed fr o m do wqild ewBin fe e ted j a Pa n e s e r a dis h r o o t 2top ah Y, 73 (3): 20 8B214. eh n ke , M . , Ln ed o n ke r , N , 2977 . Is o la t
17、i o n a n d pa r tia l e ha ar e te r is a tiwo f ph y to t o x ie s u bs ta n e e fr o m e u ltu r e if lt r a t e s o f th e fu n gu s ph y t o ph o ro i n fe s ta n s .Yd: e4 510 1. , 85: 27B27 . A e a dem ie p r e s s , L o n - d o n , N e w Y o r k , S a n F r a n e ise o . e h r , L , 1949 . n
18、I D . R . T h r elaf ll, J . F ri e n d , (ed s . ) Bio ch e7e a l A s pe e st of p la n tBpa ra site R e la tio ns hips , 1976, 2 91 . r e n e h, C . J. , Sm ith , H . , 197 5. A n in a e tiv a t o r o f p he n v la la n i n e a m m o n iaBly a s e fr o m gh e r kin g ypo e o tv ls C to chem . , 14
19、: 963B966. E IinBFo u a d , M . K . , 1956 . nI J . F r ie n d , D . R . T h r e laf ll (e ds . ), Bio eh e mi e a l A sp e e ts o f p la n tBp a r a site R e - la t io n s hiPs , 19 76, 291 . A e a de m i e P r e s s, L o n do n , N e w Yo r k , Sa n F r a n ei s e o . r r ie n d , J , R e y n o ld
20、s , 5 . B . , A v e y a r d , M . A . , 19 73 . p h e n y la la n in e a m m o n i a ly a s e , e hlo r o g e n ie a eid a n d iI g n in i n p o ta t o t u be r t is s u e in o e u la te d w ith p,OtP hrho ar A ta n s . C 5 10 1. p al n tOtho l , 3 (4 ): 4 95B507 . c eig e r , P . J、, B e ssm a n 5.
21、 P . , 19 72 . P r o te in a e te r m i n a t io o by L o w r y ,: m e t ho d in th e p r e s e n e e 。 r s uBhy d r y l r e a g e n ts An at. B fo che m . , 49: 467B463 . C r o s s , G . G . , Z e n k , M . H . , 1966. D a r s te llu n g a n d Eig e n s eh a ft e n v o n e o e n z y m e ABth io le
22、s te r n s u bt it u ie r te r 2 im ts a u r e n .aN z。4rs clt . , Zl b : 6 53B690 . H a h lbr o ek, K . , 1977 .W. aB r z , E . RYh a r d , M . H . Z e n k (e ds . ), lP a n t iT s u e C u lt u r e a n d It , 5 Bi o te e h n o lo 9 ic a ! A plP ic a tio n , 1978, 9979B9 997 . A e a de m ie p r e s
23、s , N e w oY r k , L o n d o n H ein z m a n n , U . , S ei t z , U . , S eit z , U . , 19 77 . P u r iif e a tio n a n d s u b s tr a te s p e eiif e i tie s o f h y d r o x y ein n a ma te : C o A lig a s e fr o xn a n t ho e y a n inBe ota in in g a n d a n th o e ya n i nBfr e e e a r r o t e el
24、ls . alP n ra , 135: 313B315 . H c n de sr o n , 5 . J. ,R9 75 . 1) , J . F r ie n d, D . R . hT r e lfa ll (e ds . ) , Bio e h em ie a l sA pe e st o f lP a n -t P a r as ite R e la tio n - s hip s , 1976, 2 95B298 . A e ad e m ie p r e ss , L o n d o n , N e w OY r k , S a n F r a n eis e o . e n
25、n ig e r , H . , 1959 . V e r s u e h e Z u r ku lt u r v e r s eh ie din e r r as s e n p topt。 ars ta n: (M o n t) d e baa u f kn s - tlie h en N a h r bo d e n . 2 e, top a ht . , 34 : 2 55B306. Jo h n s o n , C , A t tr idg 。 , T . , S mit h, H . , 19 75 . R6u la t io n o f p he n y la la n in e
26、 a m mo n iaBly a s e s y n t he s is by ein n a - m ie a e id: Its im P lie a tio n fo r t he ligh t m ed ia te d r e gu la t io n o f t he e n z ym e . B io c him Bop Acat , 3 85: 1 1. K o dko l, J. , C o n n , E . E , 1961 Th e m et a b o li3m o f a r o ma tie e o m po u n d s i n h igh e r pla n
27、 t W p u r iif c a tio n a n d p r o p e r ties o f t he ph e n v la la n in e dae m in a s e o f oH der u mu gl a er . . B io l. jCt e m , 236: 2692B2698 . Lmb , C . H . , R u b ew , P. H . , 1976. P h e n ylal a n in e a m m o n i aBly a s e a n d e in n a m ie a e id3-4 h y d r o x y la s e : P r
28、 o d u e t r eP rs s io n o f th e le v e l of e n z ya e tiv ity i n p o t a t o t u be r di s e s . P la n ta , (Be r l ) 13 0: 283B2 90 . L in dl , T . , K r e y z a ke+F . , Ha h lbr o c k , K . , 19 73 . Sy n ht es i s of PBe o u m a r o y l e o e n z y me a w ith a Pa r tia lly P u r i- if e d
29、 p e o u m a r a te : C o A lig a s e fr o m c e ll s u s p e n si o n e ul tu r e s o f s o yb e a n (G i n e am: ) , Bo ch“ Bop hy Ac ta , 30 2: 45 7- 464. L o w r y , 0 . H . , R o s e br o u gh , N . J , T a r r , A . L , R a n d a ll,OJ , 1951 . P r o +in m eas u r e me n t w it h th e fo lin P
30、h e n o l r e a ge n t. . B1. hC em . , 193 : 2 65B275 . V a n e e , C . P . , A v a m o o r m a n a , M . D , N a m bu dir , C . W . , T o w e r s , C . H , 1975. Is o la tio n a n d P r o Pe r t ies o f h ydr o x ye in n a m a t e : C o A liga s e , C loc hem . , 14 (4): 9 67B969 , 1:&d 43 S T UD
31、I S E ONP L A N TP H E NY L A LA NI N E A MM ONIABL YA S E(p A L ) 11. T H E R OL E OF P AL I N T H E RESI S A T N CE OF P OTA T O L A T EL IBG H T Wa n g J in gBw e n a n d Xue Y in gBlon g (D eP a tm ren t of B ioh, uF D an nUsr) T h e a e t iv ity o f PA L w a s fo u n d t o b e in e r e a se by
32、th e t r e a tm e n t o f dis e s o f a Po t a t o t u b e r w ith t h e t o x in Pr o d u e ed by . gj,0tP hht oar ifen s at n: e v e n m o r e m a r ke dly ht a n w h e n th e y w e r e in fe e t e d w ith th e p a t h o g e n s p . at o . T h e in e r e a s e o f P A L lev el w a s g r e a t e r
33、in r e sis t a n e e v a ri e ty t h a n in s u se e Ptible o n e . A s PA L a e tiv ity in c r e a s ed g r a d u a lly, a ma e r o m o le e u la r e o m Po u n d w a s a e - e u m u l d hw ieh w o u ld in tu rn in a e tiv a t e t h e PA L a e tiv ity . I t w a s d em o n s t ra - t ed tht th e e o
34、 m Po u n d w a s a th e r moBla bile, n o nBdia lyz a ble a n d dig e s tibl e by t r y Poin , hw ich w a s p r o Po s ed a s in hibit o rBPr o t ein . T h e r e w e r e diNr e n t su b s tr a t e s Pe eiif e itie s o f C o ABLig a s e fo r r e sis ta n t a n d s u se e Ptible v a ir e tie s . T h
35、e Km , 5 o f fe r u lie a e id , sin a Pie a eid a n d e a fe ie a e id w e r e 18 gM, 44 . 4M a n d 45 l ” M r e s Pe e tiv e ly of r th e r e sis ta n t v a ir e ty “ M“ pa, , , Wh e r e a s ht ey w e r e 59 . 6M, 1 14 . 1M a n d 45 . 5 ” M r e s Pe e tiv e ly of r th e s u s e e Ptible . Th e se
36、if g u re s s e em e d t o i n die a t ed th a t r e sis ta n e e v a ri e ty c o u ld sy n - t h e si z e m o r e lign in in r e s Po n s e to in fe e tio n iw th .PSJat sn . It w a s s u g g e s t ed th a t th e P AL a e tivi ty in e r e m e n t migh t b e u s ed a s a Physio l91- c a l in die a tor of la te blig h t r e sis ta n e e i n dife r e n t v air e tie s o f Po ta to e s. f f
