1、GS/GOGATZ莫良玉,吴良欢,陶勤南(v,s310029)K1:8 =95% NH4+YVGS/GOGAT(! /! )。GS、GOGAT= 、 ?# (s, 2GS/ GOGATTM。 s F,GS/GOGAT1T ; 3NH4 +#b= 3NH4+, ?51 ? )%N 3NH4+,75 l8 =NH4+#b $ l 3NH4+。 1GS/GOGAT , “y/ 、 fF/ #C 3 / ?Z,GS/GOGATZ UGSv lM8 b 3 H= NH3 i,GS4M SO 、PPTsY) M, y; dbNH3$GS;15NH4+M SO = s8GSl ?C15NS: 。 “ -X?
2、C GOGAT E 2、v 、lM8,tlM8 b 3 H=! , h ; = s8GSFd-GOGATlM8v CO2 iHq/(; $) V 3,B b 3 s; HCO2 q/,=NH3 i 64 。 t (V ,8 =iGS/ GOGAT,i O NH4+1o。GS/ GOGATNH3 H O, 3N p,O1 1-=,y7 C、N1o。 -= 1, % k b。Y %s _ (NADP-IDH)bs _ 3, L 8、= s8% (s;L 8 s _ (NAD-IDH) ?bs M- ,y79 V ?L8NAD-IDHb4。Chen/ f / 15IDH ,L 8224!g 7 YV
3、8 %,%h | Ms ,NADP-IDH|s M-= 4GS/ GOGATO9 。X ?CL 8 8i,L 8 9-= 8。t 、R = s8NADP-IDH , V ?4= s8GS/GOGATvsO 9 。 7, NADP-IDH7 38%y . ?7 3 *“ 310 , %NADP-IDH KGS/GOGATO1o, 6Oo。ParkKahnXVvs 2NADP-IDHs8y,IDH4IDH1, IDH11=Vr11 。Lancien 5NO3 -NH4 +, 、=L 8NAD-IDHRNA9F, NAD-IDH9 ?GS/GOGAT4O;-= ?Q 2N ? #8 =C/ N1M
4、f 12 。N 4 A,GS/GOGATO V 。2 2GS/GOGAT+2.1 = GS/GOGAT2.1.1 = GSGOGAT “ -X?CGS 2 T,%GS(GS1)8GS(GS2),GS21i= s8, s F1GS T,7GS11id;F =,d;FGS1 T13,14 。C3;F,GS2v ,7C4;FGS1GS2M。GS1、GS2T,GS21 ; #b 3NH4+,7GS1= 5F s ,+Y = HGS19, GS11N M, M 3 2、F;GS1 V ,7 VT8 =NH4+l 9 H “ H 3,15 。Ochs?CI (Brassica napus)GS3y,18G
5、S(BnGS2)、2%GS(BnGSR1, BnGSR2)。BnGS21; #NO3- 3NH4+,BnGSR2NM、7BnGSR1 AEy?V ( M13 。BrugiereyQl GS15FT?C, GS1 1T 15 。GS1BlyEBI ,7GS2yI ;GS 8,B Ht,s0 G7s 3,4 。GS sy0, ;、NO3-、NH4+、, s 2+s? 3 。= GS1 V ? sNH4 +,y= H 3NH4+ GS19F, V ? cq0 GSyVr。 =GS1Jr 16 。= s8GS21s;、NO 3-、NH4 +。;GS2 1, ; s8,1 cJr , = 0=v;GS2
6、mRNA (9F;Jr s e, e 3 b#;GS2 c e 9 k b 3 。V ,;GS2 c YVW,y V4 3Hq/ E 2 GS2 mRNA 3 。yV ,B323 bpc TTq0 Vy E 2= % = GUSy; 3 。;Hq/, NO3 -9GS2Vr e,7NH4+ I N=GS2 T, V4 =GS2 mRNA; 6,= s89GS2T, I N sh )Pseudomonassyringae S sPPT“4) , GS2m RNA /,=1 HGS2/ 3 。 ?Y#v =GS2;CO2 ?YV; Y、=GS2Vr3 。= GOGAT1i8, Fd-GOGAT9G
7、OGAT96%。CoschiganotV E 2glslM8?CI Fd-GOGAT2y(Glu1Glu2) 8 ,Glu1=Vr v,7Glu21Vr;lM81 Glu1,Glu2 ? Glu1,/lM8 ?; Hq/ 3 17 。Fd-GOGAT= s8,13; #NO3 - 3NH4+,7NADH-GOGAT= Fs ,1NM 18 。2252 ,:GS/GOGATZFd-GOGATFe-S, V=1%,s0 G7s;v = Fd-GOGATBHt,s0 154KDa;Fd-GOGAT s;,; V P= Fd-GOGAT9F, H #mRNA99F 4,19 。NO 3-9 ?4Fd-
8、GOGAT19 。NADH-GOGAT= ,y7 。 s0 G9M, =s0 158KDa;NADH-GOGATY A, NH4+、NO3 - V4 4,19 。2.1.2 = GS/GOGAT GS2、Fd-GOGAT=c , (= s8,#=NH4 +1 GS2/Fd-GOGAT, VYV Fd-GOGAT E 2lM8# GS2、Fd-GOGATv lM8 L 4,17 。; L 8 3NH3YV %,YV = s8$。NO3 -$“ l, f /,vsNO 3-YV !=NH4+7$GS2/ Fd-GOGAT 20 。-= V ? 1%NADP-IDHbs ,X?CL 8 021 ,9
9、 V ? 1L 8 (m2)。 Fd-GOGATv lM8; Hq/ ? 3, =GS1/ NADH-GOGAT9 VNO 3- 3NH4 +。=GS/GOGAT| V ? ;(sucrose) 23 。m2 =NH4+GS/GOGAT 22Fig.2 The GS/GOGATcycle in leave 22(Gln-!;Glu-! ;-KG-= ;Mal- T ;NR-b ;NiR-b )2.2 ?GS/GOGAT S$r ? )(rhizobia) S _ =B5 S (infectionthreads;ITs), H %YV s ?, S r ?,% )$ db %,% )Vs ?%N
10、 )8, %N (Nif)|v N 2NH3。 )8$ )8 (PBM), PBM| )8% 7, e“ 3W!s。 )8、PBM# )8PBMW bW(PBS)F 2 38, ?%N A 4,24 , ? ) S 3 P ? Nr 3。 ? )MTVB“ y$Vr,t%N -Vr,t5%NVr24 。 ?N1 $? ) S %。2.2.1 ?GS、GOGAT ?GS1 8, 、 Ht F,%,%N ?226!g 7 3,4 。 ?GS1GSn1、GSn2 s8,s0 43KDa;GSn1、 Ht,7GSn21t 4 。GS1yI 。y(gln-)$ ? ) S % =Vr,7y(gln-)5
11、 ?5“dVr 3 。?C3GSs8,2GS1(GS13、GS100)、18GS2,GS1 ?9GSvs, NH4 +1 , GS13 ?79F, ?4s8 25,26 。GS1 s ? e,%N H,GS19F。 ?,%N S gln-yVry0,7NH3 |T,v ?,I GS1(GS1-5)yVr B sNH3 e 3 。NH3 eGS1-5yVr sB400 bp F eq(SCPE) e, eqy0 27 。 ?98GS2i,NO 3-? 3,#GS2 ? b# 3 。 ?NADH-GOGAT8, ?GOGAT1 T。 ?NADH-GOGATs0 200KDa, ?2NADH-GOG
12、ATs8(NADH-GOGAT 、), (8 =28 。Trepp f%EV , ?NADHP-GOGATx8 =2 9 。 ?9 Fd-GOGAT8 =。NADH-GOGAT I$ ? ) S %,7NADH-GOGAT $ S $ S % (i 4,28 。NADH-GOGAT ?T1 ? )%N 3NH3 , s ? e,%N 7 S H, $ 6 4,28 。 ?NADH-GOGAT GS1B, V ? NH3K 28 。NADH-GOGAT#%N1 y ? 5 15% zS =Vr 29 。 ?NADH-GOGATyI ,y5 uG1(-1064)G2(-655)W188-bp, V
13、 ?YNADH-GOGAT%N u$ ? ) S % =Vr 30 。2.2.2 ?GS/GOGAT ? ? ) %N |v N2NH3, NH3 YV) PBS,PBS =$ NH4+YVPBM NH4 +0 %, PBS V ?PBM H+-ATP 。X?CPBM NH4+0,I 0yG mSAT1X$s ,0 NH4+Y+ 31 。 % =NH4+ GS1/ NADH-GOGAT 。GS1、NADH- GOGATs % u,= s8 iGln/Glu0w8 9M 0i, PGS/GOGATNH4+。GS1/ NADH-GOGAT!(Gln)! (Glu) VYV? M (AAT)? (A
14、S)sY? ?。AAT s8AAT1AAT2,AAT21s$ ? ) %x8,7AAT11s%PBM 3 2 ,ASs%。 ?GS/GOGAT V/mV U(m3)。2.3 =GS/GOGAT2.3.1 GS、GOGAT C3,GS11 =,7C4, 9MGS1;tGS2,GS2GS5% 3,4 。GS1 BH Ht 8,s0 G7s,7 OGS1 HtM1 s? H (NO3 -、NH4+)Y3 。GS1 5yEByI ,yF+s;GS1-2y15FVr,GS1-1、GS1-5 FVr,GS1-3、GS1-4 FVr 3 。l 、 CF (GS1s。,GS1(Gln1-5)K 5FVr,VG
15、S1tF3N_ 3 。GS2yI ,8 =3 。NH4 +_/GS1-3GS1-4,_ GS1-1GS1-5;NO 3- V =GS2 mRNAt 4 18,33 。v GS1 s? e 33 。2272 ,:GS/GOGATZm3 ? =GS/GOGATFig.3 Nitrogen assimilation via GS/GOGATcycle in rootnodules(PBM : )8 ;N2ase:% ;PBS : )8PBMW bW;Glu:! ;Gln :!;-KG :-= ;Asp:? ;Asn:?;AAT :? M ;NADP-1DH-NADP:s _ ;AS:? )NADH-
16、GOGAT8 =,s0 200KDa,yVr sNH4+; iNH4 + V P NADH-GOGAT mRNA #9F 34 。NH4NO3(NH4)2SO4) v H NADH-GOGAT、t#mRNA ( H9F 35 。tV NADH-GOGAT V ? s c e,9 ? “Jr e e V ?。NADH-GOGAT UV V %Vr,7 OGS19st% 36 。Hayakawa fS:0A/ NH4+NADH-GOGAT%,TV 1sV 、 %、 C%#5 C%8, U lNH4+t% = 37 。9Fd-GOGATi, O8 =, V ?yI 。 Fd-GOGAT sNO3-,
17、 m RNA8b (NiR)#%NRyVr H9F,V tyNO3 -38 。2.3.2 GS/GOGAT “ - GS/ GOGAT 。 NH4+$ lV 、 %,YVGS1/NADH-GOGATGlu、Gln, VAAT 、ASbBMAsp、Asn, !_ 。 1GlnAsn_ 。GS1/ NADH-GOGAT bWs f ?M , = s8 iQ_GluGln0w8 9iM0。 a Hq A,75 C% =9GS1/ NADH-GOGAT,y75 C%GS1/ NADH-GOGAT V ? YV5“d Gln, 3 37 。 9GS2/ Fd-GOGAT “i, NH4 + Yv,# V
18、 ? NO3- 3NH4+34 。GS1/NADH-GOGAT e V ? YVGlnT|y0,yM SO|GS1 NH4+ HNADH-GOGATmRNA ,4Gln H ,7! 、?、? 、d ( ? PNADH-GOGATmRNA 39 。 lT,lNO3 -,y7 lNNO3-, NO3 - $NRNO 2-, NO 2- 8$NiRNH4+,GS2/ Fd-GOGAT|228!g 7 NH4+Glu、Gln, VBMAsnAsp,YV !_ ; NH4+ H,GS2、Fd-GOGATmRNA (9F,V NH4+ 1YVGS1/ NADH-GOGAT 39 。3 ZN j 31,N
19、m s j。 X?CGS/ GOGAT 8 =NH4+1o, ei k b。Chapin ,9 ? l N 40 , l v| P ! M。 k9?C ? TB3 41 ,7 O Bt _ 9Y42 ; 8 = sYVGS/ GOGAT, ) !Hq/ 。! S?Z s8Cy/ 、C f / C 3 / M,9 4/ , V ?|! 4C S 。! 3 $ B ,AG Ly/ 7/r !y, P S g7/r !y4g q H1o。 ID: 1 Chrispeels M J, Craw ford N M , Schoreder J L.Proteins for transport of w a
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