1、 l : 2007- 07- 05T e : f null o ( 1986- ) , 3 , D 0 05 0 S 3 , 降香 鸡血藤b测定结果为进一步研究鸡血藤a片姜黄a降香3种中药的药物功效作用提供了可靠的数据, 为中药开发和应用提供了科学的依据b1 o M : 铁;中药; 分光光度法 m s | : O657. 31 null D S M : A null c I | : 1005- 5320( 2007) 06- 0025- 02DeterminationoftheIronintheCaulisSpatholobi, RhizomaCurcumaeLonge,LignumDalbe
2、rgiaeOdoriferaebySpectrophotometryZHANGBo, ZHAOYang, DONGShun- fu(Jilin Medical College, Jilin 132013, China)Abstract: The content of iron in Caulis Spatholobi, Rhizoma Curcumae Longe, Lignum Dalbergiae Odoriferae w as determined bySpectrophotometry. The samples w ere digested that in pH 4 6, the Fe
3、3+ w as deoxidized to Fe2+ by hydroxylamine hydrochloride, thenthe Fe2+ and 1, 10- phenanthroline produced a nacaratcomplexes. The iron content of the sample was calculated by measuring theabsorbances of sample at 510 nm by spectrophotometer. The results show that there are considerably rich iron in
4、 Chinese herbs. Theorder: Lignum Dalbergiae Odoriferae more than Rhizoma Curcumae Longe; Rhizoma Curcumae Longe more than Caulis Spatholobi. Itconcludes that the rusults give some reliable data for studying the medicinal role? of Caulis Spatholobi, Rhizoma Curcumae Longe,Lignum Dalbergiae Odoriferae
5、, which can provide some scientific data for developing and making use of chinese herbsKeyWords: iron; chinese herbs; spectrophotometrynull null S “ 0 , 0 h h “ N bT ? 0 . d 0 , “ - X W S = R 1 j b , a aY r ; aY e r ; P a e r 1bM 8 = c A , 8 = 1 3 ? , a a% # t s , 8 = = a F V , A 2b z , a a P 3 0 |
6、r , # M 3 0 0 r T 1 “ , # = c s ; E , a a P 3 0 M c b1 null L s1. 1 null N k 4N : 721s ; ; 9 , 0 s ? , Q b0 : , a a P , ( g r bk 4 : 10 nullg/ ml M S A ; 10% ( “ H ) ; 0null15% # = c ( “ H , i , 0 d ) ; 1 mol/ L NaAc A ; i H NO3aHClO4 ( ) B b1. 2 null L Z E1. 2. 1 null “ ) null “ 2 , ( b | 1null0000
7、 g, 25 ml , FHNO3 20 ml, H ClO4 5 ml h , A i , b M 25 ml , NaOH A pH 4 6,F 0 Ynull25nullnull 24 Vol. 24 null null 6 No. 6 null null null null null null null null r B Studies of Trace Elements and Healt h null null null null null null null null null null 2007 M null 11 Nov. null 2007 ,( ! b1. 2. 2 nu
8、ll | K v l o null | 10 nullg/ ml MS A 0null00a4null00 mlG Q F 50 m l , s Y G Q F 10% 1 mla1 mol/ L NaAc A 5 ml # 0null15% # = c 2 ml, F B k 4 1 ( ,F 0 Y L , 10 min, F M k 4 A T 1 A , 450 540 nm W , 5 nm B Q ; A, 500 520 nm W , 2 nm B Q ; A, V M K v l o 510 nmb1. 2. 3 null S w L null 10 nullg/ ml M S
9、 A : | 0null0702 g B ; M , , 0 , M 100 m l , 0 d Y a( , V | 5 ml A 50 ml , 0 d Y ,( b | 10 nullg/ ml M S A 0null00a2null00a4null00a6null00a8null00a10null00 ml G Q F 50m l , s Y G Q F 10% 1 mla1 mol/ LNaAc A 5 ml # 0null15% # = c 2 ml, F B k 4 1 ( , F 0 Y L , 10min, 0null00 ml T 1 A , o 510nm ) A ; A
10、b S = L 1“ z ( r = 0null999) , i 9 B Z : A =0null188C+ 0null003,M 1 “ r= 0null999b1. 2. 4 null “ null | X ) “ %! A , a a P 2null5 ml s Y 25 ml , s Y G Q F 10% 0null5mla1 mol/ L NaAc A 2null5 ml # 0null15% # = c 1ml, F B k 4 1 ( , F 0 Y L , 10 m in, 0null00 ml T 1 A , o 510 nm 3 “ A ; Ab2 null T ) 2.
11、 1 null null T# = c s ; ; E , a a P3 0 M c , T n V 1bV 1 null , a a P M c null ( mg/ g) c , 0. 221 8 0. 000 8 0. 639 1 0. 001 4 P 0. 363 8 0. 000 8null null T V : 0 M c , 3 0 c M M ,c : P , b M c K 0null639 1 mg/ g, , M c c B t 0null221 8mg/ gb2. 2 null ) null D 0 . d : , , , Y r , , , ! , A =e b ,
12、Y e r , O e , , A Z D . e , e b P , e , T , . e , = Oe , , 1bM 8 A B ,9 8 = c K ,i % = b 8 =M 9 4 5 g, 60% 70%i , 25% 30% M c M T i a , 3% , 1% c M ( % a% aV V _ )b M Y # , Y / ? a # 3 , M P % ) ? , % % ? s , V M y V r e 3, 4by N 8 =M c | B “ % h bv V , 0 r $ B , Y V s 3 x T i 9 r , “ 8 T , 0 s X 0
13、B 1 s 5b a a r 0 8= M , % h ,| Z_ b| 0 h A , pH = 4 6/ , | N M = N M , = N M # = c 3 6 , o 510 nm ) “ ; Ab Z E 0 M c ,Z E e L a 2 a z b # = c s ; ; E , a a P 3 0 M c , 0 7 ? 4 S G b I D : 1 卫生部药典委员会编.中华人民共和国药典( 一部) M .北京:化学工业出版社, 2005. 134, 186, 158. 2 张敏红,王海宝,杜有功. 铁的生物学功能与代谢调节研究现状 J . 医药导报, 2005, 2
14、4( 10) : 922- 924. 3 朱null 航,罗海吉.铁过量与人体健康 J .广东微量元素科学, 2006, 13( 7) : 9- 14. 4 吕金荣. 微量元素铁与人体健康 J .微量元素与健康研究, 2006, 23( 3) : 63. 5 张null 薇,张卓勇,施燕支,等.火焰原子吸收光谱法测定山药中多种微量元素 J .光谱学与光谱分析, 2006, 26( 5) : 963. 6 王爱荣,谷永庆,王少冷. 微量铁的分光光度测定概述 J . 广东微量元素科学, 2005, 12( 9) : 5.null26nullnull 24 Vol. 24 null null 6 No. 6 null null null null null null null null r B Studies of Trace Elements and Healt h null null null null null null null null null null 2007 M null 11 Nov. null 2007
