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类型茶多酚抗氧化机理研究现状_胡秀芳.doc

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    茶多酚抗氧化机理研究现状_胡秀芳.doc
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    1、-: 1999)03)10 : 1999)09)03:(1971),-,: 1000)369X (1999) 02)0093)11 (-310029) ,6:,-: : TS20213 : AReview on Antioxidative Mechanism of Tea PolyphenolsHu Xiufang, Shen Shengrong, Jae-il Park, Yang XianqiangDepartment ofTea Sciene, Zhejiang University,Hangzhou 310029Abstract Tea polyphenols (TP) are the

    2、 key active components of tea with high antioxidative activity. The mechanicsexpressed in six ways: adjusting the enzyme activities related to free radicals, scavenging free radicals, chelatingmetal ion,regenerating the antioxidant in organism, synergistic action with other components and improving

    3、organic immunity.Key words:Tea polyphenols Antioxidation Mechanism,A-,1,23,EBHTBHA3945,EGCGCE1816,CE,6: 1999,19(2): 93)103Journal ofTea Science1 -,6Cd,),)SODGSH-PxGSH-S-,CdCd71.1 (XO):, ATPADPAMP,;,Ca2+,O#28ESR,9, P-45010:Cho Y. J.11,XOP-45012:NADPH-P-450)ECEGCECGEGCGP-450,P-450,P-45013:,)400 nm,380

    4、 nm,(?)14P-450Wang Z. Y.14,(ECEGCEGCG)P-450,TPA (12-o-tetrade-canoylphorobo-l 13-acate),TPATP1516,17Jiro Sekiya18ECGEGCG,ECGEGCG,pH47,50%EGCG/LOX ()5,)gelatinpersimmontannisB-glucosidase-chinese sumac,01301601110%30%,20,EGCGECGEGCG, 510-5mol/L1994 19112 (SOD)(GSH )(CAT),:O#2+ O#2+2H+SODH2O2+O2CAT-Fe

    5、3+H2O2?+H2O2CAT-Fe3+2H2O+O2H2O2+SCN-OCN-+H2OROOH (HOOH) +2GSHSe-GSH-PxROH (HOH) +H2O+GSSGGSSG+NADPH+H+GSSG-Tr2GSH + NADPH+-,10: O#2CATGSH-Px, SODO#2CATGSH-Px; H2O2Cu-Zn-SOD,CATGSH-PxH2O2Cu-Zn-SOD,20,TPSODMDA60 minTP,30 min15minSOD54102%(P01001), MDA21180%(P 01001): TPGSH-Px(P 0101)SOD(P0105),(P 0101

    6、),(P 0101)21TP,0105%TP15,MDA(P0101),SOD(P 0105)CAT(P 0105)225 6SKH-?012% TP30,GSH-Px-,GSH-TrGSH-,23, TP-24TP552SODLPO,LPO, SOD,SOD/LPO25, TPTP: a) TP,10b) TP,- SH26c) TP,27TP2 :95 2:,28:O#2#OH1O2H2O2;ROO#RO#)-,211 (PMN)PMAO#2#OH,TP,TP29TPPMN,TPO#2#OHX/XO-lumino,TPO#2,TP01013 01375Lg/mlO#2,50%0104Lg/

    7、ml30TPO#290%31EGCGO#2,CEFentonTP#OH32,(010430110 mg/ml)99%,4%20%-,#OH,33,TP#OH, TPTP#OH34TP1O2135, TP1O2,2,1 1O2Table 1 Scavenging rate of tea polyphenots on 1O2 K(1O2+Phe-OH) (mol/L#s)-1 K(1O2+Phe-OH) (mol/L#s)-1C 111108ECG 212108EC 916107EGCG 212108EGC 111108636EGCG,-37:-: EGCGUTPECGECUEGC.-96 19,

    8、:,-212 ,38:TP):TP+L.TP.+LHTP+LO.TP.+LOHTP+LOO.TP.+LOOHTP70,(),TP39, TPBHA216,E312; TPBHA214,E916; EGCGE319, TPTPEGCG40NONO2TP,TPESR41: EGCG, TPBHT, TP, TP,O#2,;,TP423 TP,(TYP,)431ml 011%TYP,10 ml 50010-6,TYPZn2+, Cu2+,Mg2+, Co2+, Mn3+, TYPCa2+,Pb2+,Fe3+Fe2+,TYPAl3+,TYPCa2+Fe,Cu2+Ca2+Cu2+Fe3+Fe2+1044

    9、97 2:311 FeFe10:a) ,O#2, H2O22GSH+2O2FeGSSG+2O#2+2H+b) Haber-Weiss#OHO#2+H2O2Feton#OH+OH-+O2c) O#2Fe(FeO2+2)(FeO2+)Fe3+O#2Fe3+O#2Fe2+O2Fe2+O2Fe2+2Fe2+O (FeO2+)d) ROO#RO#2ROOHFeROO#+RO#+H2OROO1RO1,FeEGCEGCGECGECFe (III)3B22B12B13B145TPFe,Fe,46(4 mg/l)(4 mg/l), Fe0%45%,:Fe-600800TP,47TP,2114%48,EGCG01

    10、02 mmol/L, ECG01025mmol/L, EGC01028 mmol/L, EC0105mmol/L49Fe,(),Fe,Fe50312 Cu2+TPCu2+,Cu2+(LDL)51TPCu2+,TPCu2+SOD, TP,SOD313 Ca2+TPCa2+,Ca,ATP,Ca2+,TPCa2+,Ca2+,52, TPCa2+,-ATP16%30%534 ,)CEGSH,98 19,10CEGSHO#2, H2O21O2E)-,TP,411 A-(30%),A-,(1%),A-54,A-,61410-9moldm-3s-1EGCG,A-,11110-9moldm-3s-1EGCG,(51510-9moldm-3s-1),EGCG, EGCG,A-,EGCG,A-A-(TocH)-:-E55412 CTPC561959,Erofeeva N1N.Shamrai E. F.PC1 mg()C10 mg45,C5758150 mg,C,CC,C,C;,C,C +,C,C,CStaggG. V.59,CC,TPC-,TPCTPC-:413 GSH99 2:GSHGSH-PxGSH-Tr,10F3442%6,GSH-Tr

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