1、pGEX-4T-1 编号! 载体名称! 北京华越洋生物!“#$%0;?-，限 制 性 内 切 酶 酶 切 位 点 有( ?/:A.“0BCA(9:/A.9/(A.+DB( A.8BE( A 等，可转化进大肠杆菌中高拷贝表达。)*“+,%$,- 载体是一种常 用的原核表达载体，该载体多克隆位点区域含有多个常用的内切酶位点 序列，便于不同基因的克隆；且全序列中含有 E/0 启动子、*9$ 标签序 列，是一种高效的蛋白表达载体。$/0 启动子在没有诱导物存在的情况 下， 质粒上携带的/0AF 基因产物可以有效抑制E/0 启动子的转录。 可在 ?GH- 、CBIIEJ/ 等表达菌株中高表达融合有 *9
2、$ 标签的蛋白，且用凝血 酶可将目的蛋白的*9$ 标签切掉，便于下游蛋白纯化。( 稳定性)! 瞬时表达( $K/=I;J=E( 组成型)! 诱导表达( 病毒-非病毒)! 非病毒( ! “#$%ED(*9$( *K/R;$K/)U(6(MBK:/EI(QJI0K;2JQ(0/=(2J(NIJQ(MBK()KJ)/K/E;B=(BM(I/:)JI(;=()/K/JU(A=( /QQ;E;B=(*9$(;$K/)O(-(/=Q(3(:(0BN:=I(/=Q(*9$(;YKJ)O(TT(-1Z-(0BN:=(/KJ( /R/;/2J(MBK()NK;M;0/E;B=(;=(/(0DKB:/EBLK/)
3、DS(ISIEJ:(IN0D(/I(EDJ($6O(QJI;L=(ISIEJ:U( 6EJK=/E;RJS.(*NED/E;B=J(9J)D/KBIJ(2NP(:JQ;/(/KJ(/R/;/2J(MKB:(-(:(N)(EB(3(:U(6( *9$(?NP(;E(;I(/IB(/R/;/2J(0B:2;=;=L(-(:(*NED/E;B=J(9J)D/KBIJ(%?(2NP(:JQ;N:( /=Q(3(J:)ES(0BN:=I(W;ED(KJFN;KJQ(2NMMJKIU( ! “#$% 系列载体：! ()*“+,-$(“0BCAZ?6Y( ()*“+,H$( ()*“+,H$( ()*“+,
4、H$( ()*“+,7+( ()*“+,%$,-( ()*“+,%$,H( ()*“+,%$,7( ()*“+,3+,-( )*“+,3+,H( ()*“+,3+,7( ()*“+,1Y,-( ()*“+,1Y,H( ()*“+,1Y,7( ()*“+,*( ! “#$%&( 载体序列! ORIGIN 1 ACGTTATCGA CTGCACGGTG CACCAATGCT TCTGGCGTCA GGCAGCCATC GGAAGCTGTG 61 GTATGGCTGT GCAGGTCGTA AATCACTGCA TAATTCGTGT CGCTCAAGGC GCACTCCCGT 121 TCTGGAT
5、AAT GTTTTTTGCG CCGACATCAT AACGGTTCTG GCAAATATTC TGAAATGAGC 181 TGTTGACAAT TAATCATCGG CTCGTATAAT GTGTGGAATT GTGAGCGGAT AACAATTTCA 241 CACAGGAAAC AGTATTCATG TCCCCTATAC TAGGTTATTG GAAAATTAAG GGCCTTGTGC 301 AACCCACTCG ACTTCTTTTG GAATATCTTG AAGAAAAATA TGAAGAGCAT TTGTATGAGC 361 GCGATGAAGG TGATAAATGG CGAAA
6、CAAAA AGTTTGAATT GGGTTTGGAG TTTCCCAATC 421 TTCCTTATTA TATTGATGGT GATGTTAAAT TAACACAGTC TATGGCCATC ATACGTTATA 481 TAGCTGACAA GCACAACATG TTGGGTGGTT GTCCAAAAGA GCGTGCAGAG ATTTCAATGC 541 TTGAAGGAGC GGTTTTGGAT ATTAGATACG GTGTTTCGAG AATTGCATAT AGTAAAGACT 601 TTGAAACTCT CAAAGTTGAT TTTCTTAGCA AGCTACCTGA AAT
7、GCTGAAA ATGTTCGAAG 661 ATCGTTTATG TCATAAAACA TATTTAAATG GTGATCATGT AACCCATCCT GACTTCATGT 721 TGTATGACGC TCTTGATGTT GTTTTATACA TGGACCCAAT GTGCCTGGAT GCGTTCCCAA 781 AATTAGTTTG TTTTAAAAAA CGTATTGAAG CTATCCCACA AATTGATAAG TACTTGAAAT 841 CCAGCAAGTA TATAGCATGG CCTTTGCAGG GCTGGCAAGC CACGTTTGGT GGTGGCGACC 9
8、01 ATCCTCCAAA ATCGGATCTG GTTCCGCGTG GATCCCCGGA ATTCCCGGGT CGACTCGAGC 961 GGCCGCATCG TGACTGACTG ACGATCTGCC TCGCGCGTTT CGGTGATGAC GGTGAAAACC 1021 TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT GCCGGGAGCA 1081 GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA GCCATGACCC 1141 AGTCACGTAG CGA
9、TAGCGGA GTGTATAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC 1201 TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC 1261 GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC 1321 CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 1381 GTATTCAACA TTTCCGTGTC GCCCTTAT
10、TC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT 1441 TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG 1501 TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG 1561 AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG 1621 TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TT
11、CTCAGAAT GACTTGGTTG 1681 AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA 1741 GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG 1801 GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC 1861 GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGC
12、CTG 1921 CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC 1981 GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG 2041 CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG 2101 GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA 2161 CGGGGAG
13、TCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC 2221 TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA 2281 AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA 2341 AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG 2401 GATCTTCTTG AGATCCTTTT T
14、TTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC 2461 CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA 2521 CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC 2581 ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG 2641 TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCG
15、GGTT GGACTCAAGA CGATAGTTAC 2701 CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC 2761 GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC 2821 CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 2881 CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG
16、TTTCGCCACC 2941 TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG 3001 CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT 3061 TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA 3121 CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC 3181
17、GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATAAATTCCG 3241 ACACCATCGA ATGGTGCAAA ACCTTTCGCG GTATGGCATG ATAGCGCCCG GAAGAGAGTC 3301 AATTCAGGGT GGTGAATGTG AAACCAGTAA CGTTATACGA TGTCGCAGAG TATGCCGGTG 3361 TCTCTTATCA GACCGTTTCC CGCGTGGTGA ACCAGGCCAG CCACGTTTCT GCGAAAACGC 3421 GGGAAAAAGT GGAAG
18、CGGCG ATGGCGGAGC TGAATTACAT TCCCAACCGC GTGGCACAAC 3481 AACTGGCGGG CAAACAGTCG TTGCTGATTG GCGTTGCCAC CTCCAGTCTG GCCCTGCACG 3541 CGCCGTCGCA AATTGTCGCG GCGATTAAAT CTCGCGCCGA TCAACTGGGT GCCAGCGTGG 3601 TGGTGTCGAT GGTAGAACGA AGCGGCGTCG AAGCCTGTAA AGCGGCGGTG CACAATCTTC 3661 TCGCGCAACG CGTCAGTGGG CTGATCATTA
19、 ACTATCCGCT GGATGACCAG GATGCCATTG 3721 CTGTGGAAGC TGCCTGCACT AATGTTCCGG CGTTATTTCT TGATGTCTCT GACCAGACAC 3781 CCATCAACAG TATTATTTTC TCCCATGAAG ACGGTACGCG ACTGGGCGTG GAGCATCTGG 3841 TCGCATTGGG TCACCAGCAA ATCGCGCTGT TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC 3901 GTCTGCGTCT GGCTGGCTGG CATAAATATC TCACTCGCAA TCAA
20、ATTCAG CCGATAGCGG 3961 AACGGGAAGG CGACTGGAGT GCCATGTCCG GTTTTCAACA AACCATGCAA ATGCTGAATG 4021 AGGGCATCGT TCCCACTGCG ATGCTGGTTG CCAACGATCA GATGGCGCTG GGCGCAATGC 4081 GCGCCATTAC CGAGTCCGGG CTGCGCGTTG GTGCGGATAT CTCGGTAGTG GGATACGACG 4141 ATACCGAAGA CAGCTCATGT TATATCCCGC CGTTAACCAC CATCAAACAG GATTTTCGC
21、C 4201 TGCTGGGGCA AACCAGCGTG GACCGCTTGC TGCAACTCTC TCAGGGCCAG GCGGTGAAGG 4261 GCAATCAGCT GTTGCCCGTC TCACTGGTGA AAAGAAAAAC CACCCTGGCG CCCAATACGC 4321 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC 4381 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA 4441 CCCCAGGCT
22、T TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA 4501 CAATTTCACA CAGGAAACAG CTATGACCAT GATTACGGAT TCACTGGCCG TCGTTTTACA 4561 ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC 4621 TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG 4681 CAGCCTGAAT GGCGAATGGC GCT
23、TTGCCTG GTTTCCGGCA CCAGAAGCGG TGCCGGAAAG 4741 CTGGCTGGAG TGCGATCTTC CTGAGGCCGA TACTGTCGTC GTCCCCTCAA ACTGGCAGAT 4801 GCACGGTTAC GATGCGCCCA TCTACACCAA CGTAACCTAT CCCATTACGG TCAATCCGCC 4861 GTTTGTTCCC ACGGAGAATC CGACGGGTTG TTACTCGCTC ACATTTAATG TTGATGAAAG 4921 CTGGCTACAG GAAGGCCAGA CGCGAATTAT TTTTGATG
24、GC GTTGGAATT / 其他大肠杆菌表达载体： pBV221 ptdTomato pET-52b(+) pAmCyan pDsRed-Express2 pBV220 pCold-GST pColdS-SUMO pCold TF pCold IV pCold III pCold II pCold I pE-SUMO pCold-ProS2 pBAD102/D-TOPO pBAD202/D-TOPO pACYC184 pBAD/Thio-TOPO pBad/Myc-His C pBad/Myc-His B pBad/Myc-His A pBad/His C pBad/His B pBad/H
25、is A pBAD-TOPO pET-23b(+) pET-23a(+) pET-23c(+) pET-23(+) pET-12b(+) pET-12c(+) pET-12a(+) pET-11b(+) pET-11a(+) pET-11c(+) pBad24 pQE-82L pQE-81L pQE-80L pQE-32 pQE-9 pQE-16 pQE-31 pQE-60 pQE-70 pQE-40 pET-51b(+) pET-50b(+) pET-49b(+) pET-48b(+) pET-47b(+) pET-26b(+) pET-32a(+) pET-21b(+) pET-22b(+
26、) pET-14b pET-16b pET-15b pET-19b pET-20b(+) pET-21d(+) pET-21c(+) pET-21b(+) pET-21a(+) pET-24a(+) pET-24d(+) pET-25b(+) pET-27b(+) pET-28a(+) pET-30a(+) pET-42a(+) pET-43.1c(+) pET-43.1b(+) pET-43.1a(+) pET-44a(+) pET-44c(+) pET-46 EK/LIC pET-37b(+) pTrcHis2 C pTrcHis2 B pTrcHis2 A pET303/CT-His p
27、ET302/NT-His pRSET-CFP pRSET-EmGFP pRSET-BFP pGFPuv pET300/NT-DEST pET301/CT-DEST pGEM-T pBad43 pGEX-4T-3 pGEX-5X-2 pBlueScript SK(+) pG-Tf2 pG-KJE8 pGro7 pET-SUMO pSE380 pET-17b pET102/D-TOPO pCDFDuet-1 pMAL-p5x pTf16 pET-28c(+) pBluescript II SK(+) pET-30b(+) pSUMO pProEX HTc pProEX HTb pProEX HTa
28、 pKD3 pKD13 pKD46 pTYB1 pTYB2 pTWIN2 pBluescript II KS(-) pTYB12 pMAL-p5e pACYCDuet-1 pEGM-11ZF(+) pEGM-7ZF(+) PinPoint Xa-3 PinPoint Xa-2 PinPoint Xa-1 pSP73 pSP64 pTWIN1 pTYB11 pTXB1 pET-5b(+) pBad/gIII C pBad/gIII B pBad/gIII A pET-5a(+) pMal-p4X pMal-p2G pkk223-3 pkk232-8 pCYB1 pEZZ18 pBAD18 pMA
29、L-c5x pMal-p2E pMal-p2X pET-44 EK/LIC pET-43.1 EK/LIC pET-41 EK/LIC pMal-c4X pTrcHis B pET-31b(+) pET-3b(+) pET-41a(+) pGEX-3X pGEX-4T-2 pETDuet-1 pGEX-4T-1 pTrc99a pET-28b(+) pET-His pALEX a,b,c pACYC177 pBR322 pKD4 pKD20 pMXB10 pEcoli-6xHN-GFPuv pKJE7 pRSET B pGEX-KG pGEX-2T pRSFDuet-1 pCOLADuet-1
30、 pTrcHis C pTrcHis A pET-41b(+) pET-42b(+) pET-3a(+) pGEX-6P-3 pGEX-6P-2 pGEX-6P-1 pGEX-5X-3 pGEX-5X-1 pGEX-2TK pRSET A pMal-c2G pMal-c2E pMal-c2X pRSET C pQE-30 pET-45b(+) pET-44b(+) pET-42c(+) pET-41c(+) pET-40b(+) pET-33b(+) pET-39b(+) pET-32 EK/LIC pET-32 Xa/LIC pET-32c(+) pET-32b(+) pET-30 Xa/LIC pET-30 EK/LIC pET-30c(+) pET-29c(+) pET-29b(+) pET-29a(+) pET-24c(+) pET-24b(+) pET-24(+) pET-23d(+) pET-11d(+) pBad33 (
