1、null8 null null_ k + v ) _ Z E # “ 5 N杨军霞, 刘贵建( S D S L y , Enullmail: null null + v ) B n | ) , 100 d, / V i 1 M b h 4 ,1% p 0 A ) s V b ) ) , 1 2. 5% 5.0% CO2 ;K a 35 36 ! ,K a pH 6. 7 7.0, 25 ! 40 ! 9 V 3 , 3 , !1 p N Y , Q s A Lnull M 0 , null A j ! (buffered charcoalnullyeast extractagar, BCYEa
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5、e L a y ,95. 0%)b2.4null 血清抗体检测null T _ + v ) “ 5 m,_ b F + v ) IgM # IgG F 8 V S + s bIgM F 8 C * , 8 1 , V _ b + s F 8 IgMbIgG F 8 C , V 8 = , 8 2 V _ IgG, V h b H , $ z b, F 8 6 r $ 4 , O r 1#128 H b Znull2898null Chin J Nosocomiol Vol.20 No. 18 2010E :W f ; E ( IFA)a “ k (MAA)a k 5 “ k ( TAT)aEL
6、ISA E b “ - “ 5 K 99.0%), V % h * _ k + v ) b b+ s F 8 Z E g ,_ T VL , “ - S = “ 5 _ k + v ) h Z E ,9 i B t : ( 1) P ! E 9 20.0% 30. 0% F 8 1#128), 1 F 8 A 6 ,# b C * i l b2.5null 尿抗原的检测null M ,S = R _ U F Z E b_ U A _ k + v ) F B e L a y a 6 Z E b Z E b f _ E( RIA)a f _ E ( EIA)a f _ E ( ICT) ; F 8
7、 E (DFA) b t _ Z E F 8 v LP1,# ? LP1 U F _ b“ - 3 _ k + v ) U F _ k 4 ,1 :Bartela EIAaBinax NOW ICTaBinax EIAaBiotest EIA bBartela EIA k 4 _ U + v ) LP1 F 74.1%,+ s 100.0%, U A i r V ) , V r 91.5%7 bICT B _ + v ) y Z E , Te L ,15 min _ , “ - S Binax 3 Binax NOW ICT k 4 ,_ F LP1 F , Z E _ U F , i U 3
8、7. 0%,i U 69.9%,+ s ( 100.0% 8 bDFA E F ; S : F + v ) F 8 “ 5 S T ,4 + v ) b E T e L , , H ) Q b Z E _ + s ( 100. 0%, s v bs y , I n : (1) b ,_ 9 b( 2) U A V i b( 3) F ) 0 b( 4) U A S =| H W ( V / s bU F _ :( 1) _ k + v ) h ?h 1 3 d V V U _ _ k + v ) F , * 4 G b(2)e L a y a , 1 L N ! ! , T / 1 p b(
9、3) U S , 7 b U F _ 9 i B t : (1) U F H WV , E ; b( 2) “ - S = 3 E a L g aN L , “ - 4 R b( 3) a _ LP1, b _ b2.6null 分子生物学检测2.6.1null / null _ Q , a ; ab 3 S : X + DNA ( ) , B H q / , a 5 , $ S ,Y V | _ ,V 7 k S M h 3 y # s0 v l b y y S : 7 s b S: d b S : ,d b S : 3 S : a S : ; S : b S % h e X | DNA /
10、 + v ) s B ? ZE , E + s ( , B $ ,Edelstein 9 ! E + v ) 3 S _ , 1 “ ! | E ! S , 76. 0%, + s 100. 0%b , T 4 O , “ - g N Z E w k 5 ,E 9 L i L ? 3 , “ - ) H W , 8 PCR s , + s b E V D + v ) S , H9 “ 5 P h * y _ k Y bJoly 18 L H PCRZ E % ) ! E 92z “ d “ _ , T V ,% ) ! E q 44. 6%, L HPCRE q 82. 6%, , 60. 9
11、% V bB ? K K 250 CFU/ Lb N V n , L H PCR E q ! E b/ B C y V ? P h 4 “ i v E ! + v ) b LH PCRE i , :+ s Q b P s 0 3 1 bV _ k + v ) $ ? C 20 W 90 M ,v L i P _ Z E % ) ! E b _ E b% ) ! E _ _ k + v ) S , ! H W a ! N L O 1 p L / y +v ) h q q b b _ E 1_ IgG F 8 ,i ,# b C * il b L i _ Z E , U F _ 1 X _ Z E
12、 , Z E F 8 v LP1,# ? LP1 U F _ b T UF _ 9 V H _ b ,| _ Z E B v b PCR _ S , 4 _ ZE + s b H F v S k 4 L ,| v 4 L i _ b I D 1 null Scatuno M, Losardo M, Deponte G, et al . Comparison ofthree molecular methods used for subtyping of Legionellap neumophila strains isolated during an epidemic of Legionelnu
13、lllosis in RomeJ. Clin Microbiol,2005,43(10):5348null5350. 2 null EdelsteinPH. LegionnairesdiseaseJ. ClinInfect Dis,1993,16(6):741null747. 3 null Maiwald M, Helbig JH, Luck PC. Laboratory methods forthe diagnosis of Legionella infectionsJ. J Microbiol Methnullods,1998,33(1):59null79. 4 null f , y .
14、+ v h / Z J. 7 3 h % h ,2003,1(3):131null134. 5 null , 1 , V , . + v ) e _ / y J. D D ,2000,6(1):100. 6 null Edelstein PH. Detection of antibodies to Legionella sppA.Rose NR, de Macario EC, Folds JD, eds. Manual of clinicallaboratory immunologyM. Washington, DC: AmericanSonullciety for Microbiology,
15、1997:502null509. 7 null Dominguez J, Gali N, Blanco S, et al. Assessment of a newtest to detect Legionella urinary antigen for the diagnosis oflegionnairesdiseaseJ. Diagn Microbiol Infect Dis,2001, 41(4):199null203. 8 null Guerrero C, Toldos CM, Yague G, et al. Comparison of dinullagnostic sensitivi
16、tiesof three assays(Bartelsenzyme immunonullassay (EIA), Biotest EIA, and Binax NOW immunochromanulltographic test) for detection of Legionella pneumop hila seronullgroup 1 antigen in urineJ. J Clin Microbiol,2004, 42(1):467null468. 9 null EdelsteinPH, BryanRN, Enns RK, etal. Retrospective study ofG
17、ennullProbe rapid diagnostic system for detection of legionellae infrozen clinical respiratory tract samplesJ. Clin Microbiol,1987,25(6):1022null1026.10 null StarnbachMN, Falkow S, TompkinsLS. Speciesnullspecific denulltectionof L egionella p neumop hila in water by DNA amplifinullcationandhybridiza
18、tionJ.ClinMicrobiol,1989,27(6):1257null1261.11 null Engleberg NC, Carter C, Weber DR, et al . DNA sequence ofmip, a Legionella p neumop hila gene associated with macronullphage infectivity J. Infect Immun,1989,57(4):1263null1270.12 null Ng DL, Koh BB, Tay L, et al . Comparison of polymerasechain rea
19、ctionandconventionalculture for the detection of lenullgionellae in cooling tower waters in SingaporeJ. Lett ApplMicrobiol,1997,24(3):214null216.13 null k , _ , t , . Q _ + v ) J. “ 5 _ ,1995,13(5):230null231.14 null R + F , k = , , . + v ) PCR_ Z E y J. S 3 _ ,1997,7(6):347null349.15 null n j , , ,
20、 . + v ) PCR _ Z E y # J. S u h ,2000,16(5):13null16.16 null , S o , d , . PCR _ 8 + v ) J. 3 f Z ,2005,33(3):45null48.17 null Z k d , k ,f . Q _ b _ k + v ) DNAJ. C D ,1999,26(4):419null420.18 null Joly P, Falconnet PA, Andre J, et al . Quantitative realnulltimeLegionella PCR for environmental water samples:data internullpretation J. Appl Environ Microbiol, 2006, 72( 4): 2801null2808.欢null 迎null 投null 稿null null 欢null 迎null 订null 阅nullnull2900null Chin J Nosocomiol Vol.20 No. 18 2010
