1、 Chinese Bulletin of Botany 2012, 47 (3): 278285, doi: 10.3724/SP.J.1259.2012.00278 iiiiiiiiiiiiiiiiii l : 2011-09-13; s : 2012-02-08 “ : SE1 S (No.31071342)av S/7 (No.2011CXJ080) 8T S “ * YTb E-mail: x X L m 9 xZE) 1, 2, y 1, i1*1v ?yF L i / 8TL. 3 L i , 225009 2v k , 225009 K1 x A-a B- C- 38b (O
2、ryza sativa)a . (Solanum tuberosum)a (Pisum sativum) (Nelumbo nucifera)x , x X- L N (XRD) 8 xo + , ) XRDo M9 ZEb q EEa q wLEaLTmE wLTmE ( V9 x XRDo M , wLTmE9 T V Lb wLTmETV , x xc ALM1 , xx ALM1b P x C-8M A-8b T XRDsx8 9 M4 1 Ib 1oM , x , x X- L , y , i (2012). x X L m 9 xZE) . 47, 278285. x 1 ? ,
3、9 1 J 1 , Bruker D8 XRDsb kHq : 40 kV, 200 mA, (2)S 340, s 1.2, 0.02b “ 30sb 1.6 x XRDo M9 x XRDo , IM1D , 4?ZE9 xM (Nara and Ko-miya, 1983; f , 2001; Huang et al., 2007; , 2010; g , 2011)b ZEB : q1 E9 XRDo , e q EEb XRDs q MDI Jade 5.09 Mb T / : P Jade 5.0 qu 7“ XRDo , | 2 430S =m , 4 30WL ; F , q1
4、o E ; F , Q q1o Q E ( EV , 2 17vM , 2 23 v9FbtTV , C- xvM A-8 , C- x A-81 B-85 ( y )(m 6)b Yu (2009) C- x 284 47(3) 2012 TMBb wLTmE , xs , TV , , xMA4 (V 3)bd9sTV , xMx ALM1 (R= 0.94), V xs1s b 3 x A-a B- C-8 XRDob q EEa q wLEa wLTmELTmE x XRDo 9 s ZEb q EEeaZL O y , TA , axb q wLE VZL y x , u 9$9 u
5、Bs , #74 bLTmEe sL , 9T V ? LM b wLTmE sL# u u 9Z TN , 9 ms q9 ?y , i O9 T1 V L , $WxXRDo 9 sb wLTmE9 xMTV , x xc ALM1 , xx ALM1b ID g , f , (2011). x9 ZE . J S 32(9), 6871. , f , SZ , u , f_ (2010). X Lx R . J S 31(3), 284 287. f , f , 3 , (2001). x8“ZE . 2 v (1 S ) 29(5), 5558. Cheetham NWH, Tao L
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13、ydrolysis method. Food Chem 113, 585591. : x X L m 9 xZE) 285 Methods for Determining Relative Crystallinity of Plant Starch X-ray Powder Diffraction Spectra Bin Xu1, 2, Jianmin Man1, Cunxu Wei1* 1Key Laboratories of Plant Functional Genomics of Ministry of Education and Crop Genetics and Physiology
14、 of Jiangsu Province, Yangzhou University, Yangzhou 225009, China; 2Testing Center, Yangzhou University, Yangzhou 225009, China Abstract Plant starches have A-, B-, and C-type crystallinity. We investigated spectrum characteristics of starches from rice, potato, pea and lotus by X-ray powder diffrac
15、tion (XRD). Peak-fitting, curved-line, straight-line mapping and curved-line mapping methods are commonly used for determining relative crystallinity of starch XRD spectra. The curved-line mapping method has reliable results. We found the relative crystallinity of rice starch negatively associated w
16、ith amylose content and that of acid-modified lotus starches positively associated with acid hydrolysis degree by the curved-line mapping method. The crystallinity of lotus starch changed from C- to A-type during acid hydrolysis. These results would be useful for XRD analysis of crystallinity and re
17、lative crystallinity of plant starches. Key words crystallinity, plant starch, X-ray powder diffraction Xu B, Man JM, Wei CX (2012). Methods for determining relative crystallinity of plant starch X-ray powder diffraction spectra. Chin Bull Bot 47, 278285. *Author for correspondence. E-mail: (3 I : )
