1、cI|:1006-3110(2010)10-2131-04 【综 述】5- )Y Z李 影,穆 萨,唐爱国K1: 5- )Y (5-HIAA) (TRP)-5- (5-HT)o,5-HT (MAO)T/7 。 88A5-HIAAc %h、 # 1N,5-HIAA 3+、ZE# “5il 8 。1oM: 5-羟吲哚乙酸;5羟色胺;色氨酸;类癌ms |:R311 DS M :A5- )Y (5-hydroxyindole acetic acid, 5-HIAA)Y 3, )Y s,|5-HIAA y 3p。1.2 TRP-5-HT代谢途径的主要酶1.2.1 TPH TPH TRP5-HTK ,9
2、K1 。TPH1O2 、Fe2+# _ 3,7 = c , , 5-HIAA 3K 。TPHs TPH1TPH2。TPH11s#、k、#、 E= / F,9s 7 =+uVr,1 e5-HT;TPH2 *+s, 7 u5-HT *Vr3 。 TPH1y 811p15.3-16 u4 ,TPH2y 812q15 u3 。TPH2TPH1, 71% 5 。TPH1 ? TPH2 ( =, 58 (Ser58) (PAK),Ser58Ser260 G - II。TPHyI TPH,yNTPH1yTPH2y ? 3Ms, MTPH,5-HIAA“d ? y,V7 P5-HIAAM。KSV,TPH281
3、 ? 316 。1.2.2 AAAD AAADTrp-5-HToi K , h 7 = *,AAAD bL-DOPA_K , AAADYVK h 7 p 51(Carbidopa),V7KL-DOPA_*。 H,AAAD9 5-HTP_5-HTK ,N2131 LD 2010 M10 17 10 Practical PreventiveMedicine,Oct.2010, Vol 17, No.10)AAAD? 3hM, V ? *? 3。I AAAD y$DDC, 87| 87 。C?C,y? 3Ms * *p? 31。 :Ba-601? 3 BA0 a722-725? 3 _ fp1? 3
4、1。1.2.3 MAO MAO B ?b Q ,K*1928 MHare%?C,i| “ ” 。MAOi8v %L 8 。8 =s “1T, MAO ?p$ *“d%h1。 :、 *s 、i 、 he8MAO s。yN, TRP-5-HTo iB C,5-HIAAp,i B“ y。2 5-HIAAZE5-HIAA 5-HT1, VQ8 =5-HT ,9 VTTRP8 = S:B。8A5-HIAAZE11 E、;sE、rAM E(HPLC)、 E(MS)ZE。2.1 比色法 Udenfriend1y bMp1 E U5-HIAA。 n5 | UA (pH ,M 。2.2 荧光分析法 George
5、 G 11 n5 0 b5-HTP、5-HT5-HIAAs ,F F k4 P ? 95%TRP%3IDOb,J U 。90%TRP,10%TRP 。% H,F ,/, 9F, ( V TRP9F,F| 5-HIAA U 。 U5-HIAA1 A9, A。?C31 24 h U5-HIAAc 、 、GQ 6。 ? F, U5-HIAA 6。5-HIAAc L ? S、 (Bil)M1。3.8 急性阑尾炎早期诊断 $ KnB%h, ? MY d 4, lY 。.dZE ql80%,yN?CB y 、ZE d1。V , W, _% db5-HT5-HIAA$ M1“。Hernandez R 32,
6、64 ,52 $ U5-HIAAc 19.31 mol/L,7 23.10mol/L,7vF17.27 mol/L,$ U5-HIAA A9, O9,yN U5-HIAA VT$ B 3S。3.9 估计酒精的摄取量 4 U,0 v 7 *M, HW、v 7 *. M,v 7 aM, V ? B us8h ,9 V ?h t * 3。 V Pv7 *5-HTs,VC5-HT h。 5-HTs, 7,A5-HIAAc , V w *“d5-HT w 。5- - (GTOL)5-HIAA1(GTOL/5-HIAA)z VT9 | S: 33 。7.dS |_E,KZ T YV 8、A UAc , 8
7、 = y, ? c ,yN1B8 = HW 3S,yGTOL/5-HIAA19FV 8 = 9F,i O HW,yNGTOL/5-HIAA VT SB。3.10 其他 Kirillova34,、l5-HT5-HIAAc =UhMk 1, H k 5-HT5-HIAAc M1。N, / ?s、&d U、 1、JF ? G、 5F ?F、 lp%h g Whipple %h95-HIAA M。8 ,5-HIAA 8 =1 *,9WQ8 = th M。 H “ _2133 LD 2010 M10 17 10 Practical PreventiveMedicine,Oct.2010, Vol 17,
8、 No.10ZE m?Z,5-HIAA “5M1%h|BZ,5-HIAA| “5 4 V LG 。 ID1 Udenfriend S, Titus E, Weissbach H.The identification of 5-hydroxy-3-indoleacetic acid in normal urine and a method for its assay J .J BiolChem, 1955,216(2):499-505.2 PageIH, Corcoran AC, UdenfriendS, et al.Argentaffinomaas endocrinetu-mor J .Lan
9、cet ,1955, 268(6856):198-199.3 Zill P, Baghai TC,Zwanzger P,et al.SNP and haplotype analysis of a noveltryptophan hydroxylase isoform(TPH2)gene provide evidencefor associationwith major depression J .Mol Paychiatry, 2004, 9(11):1030-1036.4 AbbarM , Courtet P, Bellivier F, et al.Suicide attempts and
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11、on, and cerebrospinal fluid 5-hydroxyindoleaceticacid in 4 populations J .Arch Gen Psychiatry, 2005 ,62(10):1109-1118.7 Scherer LJ, McPherson JD , Wasmuth JJ, et al.Human dopa decarboxylase:localization to human chromosome 7p11 and characterization of hepatic cD-NAs J .Genomics,1992, 13(2):469-471.8
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13、23-34.10 Harry Goldenberg.specific photometric determination of 5 hydroxyin-doleacetic acid in urine J .clin.Chem, 1973, 19(1):38-44.11 George G, Guilbault, PeterM , et al.New Assay for Tryptophan and Its 5-HydroxyindoleMetabolites in Blood J .Clin.Chem, 1974,20(7):812-815.12 Santagostino G , Fratti
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18、s spectrometry J .J Chromatogr BAna-lyt Technol Biomed Life Sci, 2008,868(1-2):28-33. 19 Allen KR, DeggTJ, Anthoney DA , et al.Monitoring thetreatment of car-cinoid disease using blood serotonin and plasma 5 -hydroxyindoleaceticacid:three caseexamples J .Ann Clin Biochem, 2007, 44(3):300-307. 20 sbe
19、rg M, Neurotransmitters and suicidal behavior.Theevidencefrom cere-brospinal fluid studies J .Ann.N .Y.Acad.Sci ,1997, 836:158-181. 21 Stanley M, Mann JJ.Increased serotonin-2 binding sites in frontal cortexof suicide victims J .Lancet, 1983,1(8318):214-216. 22 Nordstrm P, SamuelssonM , sberg M, et
20、al.CSF 5-HIAA predicts sui-cide risk after attempted suicide J .Suicide Life-threat Behav, 1994, 24(1):1-9.23 b, d, o.s - *M J .D0, 2009, 40(5):35-38. 24 AndreaG , WelchKM, RiddleJM , et al.Platelet serotonin metabolism andultrastructure in migraine J .Arch Neurol, 1989, 46(11):1187-1189.25,B, ,.5x
21、he#l5 -HT、5-HIAAc M J . “5 *h, 2000, 13(6):355-356. 26 Wi sniewska-Jarosiska M , Boznaska-Swietaszek P, Harasiuk A , etal.24-hour urinary 5-hydroxyindole aceticacid excretion in patients withulcerative colitis J .Pol Merkur Lekarski, 2009, 26(155):452-454. 27 Moskwa A, Chojnacki J, Wi siewska-Jarosi
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23、n infantile hydro-cephalus ChildsJ .Childs Nerv Syst , 2008, 24:713-716. 29 Gopal SC, Sharma V, ChansuriaJP, et al .Serotonin and 5-hydroxy indoleacetic acid in infantilehydrocephalusJ .PediatrSurgInt , 2007, 23(6):571-574. 30f , ,0,. ( %h# 385-HT5-HIAAc M#il J .S r,2008,23(13):1871-1872. 31 , a,f,.
24、5- )Y ? “5N J .h, 2005,25(5):303-304. 32 Hernandez R, Jain A, Rosiere L, et al.A prospective clinical trial evalu-ating urinary 5-hydroxyindoleaceticacid levels in the diagnosis of acuteap-pendicitis J .Am J Emerg Med, 2008, 26(3):282-286. 33 Stephanson N , Helander A, Beck O .Alcohol biomarker anal
25、ysis:simulta-neous determination of 5-hydroxytryptophol glucuronide and 5-hydroxyin-doleaceticacid by direct injection of urine using ultra-performance liquidchromatography-tandem mass spectrometry Journal of mass Spectrometry J .J.Mass Spectrom, 2007, 42(7):940-949. 34 KirillovaVV , Nigmatullina RR
26、, Dzhordzhikiya RK, et al .Increased con-centrations of serotonin and 5-hydroxyindoleaceticacid in blood plasmafrompatients with pulmonary hypertension due to mitral valve disease J .BullExp Biol Med, 2009, 147(4):408-410. ( l :2010-07-22)2134 LD 2010 M10 17 10 Practical Preventive Medicine, Oct.2010, Vol 17, No.10
