1、2013 年度本科生毕业论文超临界 CO2 萃取海南黎药麦奋中挥发油有效成分的工艺研究学 院： 理工学院 专 业： 化学 2013 年 5 月2013Annual Graduation Thesis of the College UndergraduateSupercritical CO2 extraction of volatile oil and effective constituents in Hainan li medicine MaiFen of process researchDepartment: School of Science and TechnologyMajor: C
2、hemistryGrade: 2009Students Name: xxNo.: xxxxTutor: xxxFinished by May, 2013毕业论文（设计）原创性声明本人所呈交的毕业论文（设计）是我在导师的指导下进行的研究工作及取得的研究成果。据我所知，除文中已经注明引用的内容外，本论文（设计）不包含其他个人已经发表或撰写过的研究成果。对本论文（设计）的研究做出重要贡献的个人和集体，均已在文中作了明确说明并表示谢意。 作者签名： 日期： 毕业论文（设计）授权使用说明本论文（设计）作者完全了解琼州学院有关保留、使用毕业论文（设计）的规定，学校有权保留论文（设计）并向相关部门送交论文（
3、设计）的电子版和纸质版。有权将论文（设计）用于非赢利目的的少量复制并允许论文（设计）进入学校图书馆被查阅。学校可以公布论文（设计）的全部或部分内容。保密的论文（设计）在解密后适用本规定。 作者签名： 指导教师签名：日期： 日期： xxx 毕业论文（设计）答辩委员会(答辩小组)成员名单姓名 职称 单位 备注琼州学院本科毕业论文摘 要海南黎药麦奋（别名异形南五味子、海风藤、 梅花钻、 过山风、风藤） ，中国药典记载海风藤为胡椒科植物风藤，它的藤茎，具有祛风湿、通经络的功能，而植物风藤的分布范围狭窄且资源极度匮乏。同时中医药是中华民族优秀文化的重要组成部分，是中国的国粹。据调查而知，植物界中挥发油分
4、布范围很广，有数百种含有挥发油的植物已作为中草药来使用，挥发油也称精油，是植物体内的次生代谢物，由相对分子质量较小的简单化合物组成，具有芳香气味，在常温下可挥发，可随水蒸气蒸馏出来的油状液体的总称。在水和浓度低的乙醇里，其溶解性比较差，不能大量溶解，但在无水乙醇里，可完全溶。它一般易溶于氯仿、石油醚、乙醚、油脂、二硫化碳等有机溶剂；一般挥发油对空气、日光和温度都较为敏感，易变质，因此，应放在棕色瓶内避光低温密闭保存。由此本论文将采用近年来发展最为迅速，最引人关注的超临界 CO2 萃取、分离技术。且超临界 CO2 在提取天然产物，具有很多优点，如有较好的萃取、分离效能，集萃取、分离、浓缩为一体，
5、整体操作成本低，无残留剂、无污染、节约能源、操作方便等。现超临界 CO2 萃取技术已广泛应用于香料、食品、医药、石油、化工等领域，并受到工业界的普遍重视。用此技术为海南黎药麦奋的挥发油深加工和综合利用寻找一条有效途径。方法：本文采用超临界 CO2 萃取法提取海南黎药麦奋中挥发油有效成分的工艺研究，主要考察了萃取温度、萃取压力、时间和 CO2 流量等影响因素。结果：萃取压力和低温对海南黎药麦奋中挥发油含量的影响具有显著性意义，同时也由于条件的限制，故此整个实验都以 CO2 流量 15mL/min 为标准来进行实验。实验表明，采用超临界 CO2 萃取法提取海南黎药麦奋中挥发油的最佳工艺条件为：药材
6、粉碎过 20-30 目标准筛，萃取压力 15MPa，萃取温度 37，时间 2h。关键词：超临界 CO2；海南黎药麦奋；挥发油；单因素实验；正交试验琼州学院本科毕业论文ABSTRACTHainan li medicine MaiFen (alias abnormity kadsura pepper stem, the plum flower drilling, wind, wind cane), Chinese pharmacopoeia record for pepper pepper stem families wind cane plant, its stem, with function
7、s of wet, the function of the channels and collaterals, and narrow distribution range and wind cane plants resources extremely deficient. At the same time is an important part of the Chinese nation excellent culture of Chinese medicine, is the quintessence of China. Known according to the survey, th
8、e plant community in the volatile oil distribution range is very wide, there are hundreds of different plants have been used as Chinese herbs containing volatile oil, volatile oil, also known as essential oils, the plant secondary metabolites in the body is composed of smaller relative molecular mas
9、s of simple compounds, aromatic smell, can be volatile at room temperature, with the steam distillation of the floorboard of the oily liquid. In low concentration of ethanol and water, its solubility is poor, cant dissolve in great quantities, but in anhydrous ethanol, can be completely dissolved. I
10、t usually soluble in chloroform, petroleum ether, ether solvents, oils, carbon disulfide, etc; Generally the essential oil is sensitive to air, sunlight and temperature, perishable, therefore, should be put inside brown bottle dark airtight preservation at low temperature. Thus this thesis will use
11、the most rapid development in recent years, the most noteworthy of supercritical CO2 extraction and separation technology. And supercritical CO2 extraction of natural products, has many advantages, such as good extraction, separation efficiency of extraction, separation and enrichment, the overall o
12、perating cost is low, no residue, no pollution, energy saving, convenient operation, etc. Now the supercritical CO2 extraction technology has been widely used in spices, food, medicine, petroleum, chemical, and other fields, and has been widely promoted by the industry. Using this technology for vol
13、atile oil deep processing and comprehensive utilization of Hainan li medicine MaiFen to find an effective way. Methods: This article USES the Hainan li medicine extraction by supercritical CO2 extraction of volatile oil and effective constituents in MaiFen technology 琼州学院本科毕业论文research, mainly exami
14、nes the extraction temperature, extraction pressure, time and CO2 flow influence factors.Results: The extraction pressure and temperature on the influence of the volatile oil content in the Hainan li medicine MaiFen has significance, but also because of the constraints, so the whole experiment based
15、 on the CO2 flow 15 ml/min to experiment. Experiments showed that the supercritical CO2 extraction method to extract volatile oil of Hainan li medicine in pepper stem the best process conditions for: 20-30 goals have medicinal materials crushing sieve, 15Mpa extraction pressure, extraction temperature of 37 , time of 2h. Keywords: Supercritical CO2; Hainan li medicine MaiFen; Volatile oil; Single factor experiment; Orthogonal test
