1、0a00c 王宗权,贾继明* ,宋剑,李正杰,孟作环( 4D0, FE 050035)K1 “:HPLC- ELSDE00a00c bZE: ZORBAX EclipseXDB- C18(250mm 416mm, 5Lm), MY ,0!, 1mL# min- 1,35 e ;?; _ , ELSD : 39e , M555 e , 17214kPa,9m200bT:0a00LSsY018312 81312Lg( r= 019996), 21544 161536Lg( r= 019990), 013283128Lg(r= 019992); ( l qsY9911% , 9810%, 9816%
2、,RSDsY319%, 218%, 218%b :ZEe y,a00c ,sD4 G b1oM:HPLC- ELSD;0ms |: R917DS M : AcI|: 0254- 1793(2010)07- 1191- 04Determinationofastragaloside, astragaloside andastragaloside inRadixAstragalifrom varioushabitats*WANG Zong- quan, JIA Ji-ming* , SONG Jian, LIZheng- jie,MENG Zuo- huan(HebeiYilingMedicine
3、Institute,Hebei050035, China)Abstract Objective:To establish anHPLC-ELSDmethod for the determination of the contentof astragaloside,astragalosideandastragaloside inRadixAstragalifrom varioushabitats.Methods:Theconditionsofchromatogra-phy: ZORBAXEclipseXDB- C18(250mm 416mm, 5 Lm)was usedwith amobile
4、phase ofCH3CN-H2O, flowrate: 1mL# min- 1, column temperature: 35 e ,: spray temperature39e , drift tube temperature: 55 e , airpressure:17214kPa, gain: 200.Results:The calibration curves of astragaloside, astragaloside andastragaloside were ingood linearity overthe ranges of018312 The average recove
5、rieswere 9911%, 9810% and9816% withRSD of319%,218% and218%, respectively1Conclusion:Themethod is simple, rapid, sensitive and reproducible fordeterminationofastragalosides inRadixAstragal.i and canbeusedabasis for findingbetterhabitatofRadixAstragal.iKeywords:HPLC-ELSD;RadixAstragal;i astragalosideS
6、E$?Z9 “( 2005CB523301); / EB0SE S/9( 2006BAI08B04- 09)S= S/T9 “( 2008DFA30810); /v070 S/v( 2009ZX09102-140)*YT Te:l ( 0311)85901304; E-mai:l jjm_0451 163. com S A stragalusmembrana-ceus(Fisch. )Bge. var. mongholicus (Bge. )Hsiao GA stragalus membranaceus (Fisch. )Bge.2, %Va Ua ha 2 3r 1bSsW,1s Ca a
7、= aa b MV ,1c0 , F 2a f ?3a 4a Fh5 756Z ATb“ -10c 1 E7aHPLC-UVE 8aHPLC-ELSDE 9 0c ,7 0b kHPLC- ELSDE03100a00c ZEb1 N k0Waters2695rAM N, 2420?; _ (Waters ), Empower2 T_(Waters)1191)0sChin JPharm Anal2010, 30(7) ), Milli-QB ( SM illipore ),AM Y B, k4sBb01S0 3 _ ,00 ZZ 3 S/K ( NMRo / k ,HPLC BBE_,B (v9
8、8% )b0=1a = a b CD0v qk As-tragalus membranaceus (Fisch. ) Bge. var. mongholicus(Bge. ) Hsiao GA stragalus membranaceus(Fisch. )Bge.2, 60 e,x V4| b!b2 ZET211 A!21111v A! sY |=h20a00v a ,FJ? sY 014156mg# mL- 10v A,11272mg# mL- 10v A#010656mg# mL- 10v Ab21112 k A! | x4g, f4 | ,FJ? a , V,FJ?70mL,F 4h,4
9、 |A l 4,?F70%J? A,M10mL ,F70%J? A d Y,( ,!b212 Hq : ZORBAX EclipseXDB-C18 (250mm 416mm, 5 Lm); M:AMY, BM ,0!, 5%A(0 10min), 5% y 24%A(10 40min), 24% y 34%A(40 70min), 34%A(70 80min), 34% y 60%A(80 95min), 60% y100%A(95 100min), 100%A(110 115min); : 1mL# min- 1;: 35 e ;?; _ , ELSD : 39 e , M555 e , 1
10、7214kPa,9m200;“81020LLb mnm1bm1 v (A, B, C)0“ (D)HPLC-ELSD mFig1 HPLC-ELSD chromatograms ofreferencesubstances(A, B, C) andRadixAstragali(D)1.0( astragaloside) 2.0 ( astragaloside ) 3.0 (astragaloside )213 L1“ I sY |0v A2, 6, 9, 11, 13, 20 LL,0v A2, 4, 8, 12, 13 LL,0v A5,9, 13, 30, 50 LL, rAM N, ,sY
11、0a00v“ (X )1 US, s(Y)1 :US,S wL,9 BZb0a0a0BZsY:Y= 11637X+ 1213 r= 019996Y= 11479X+ 12171 r=019990Y= 11767X+ 12165 r=019992)1192)0sChin JPharm Anal2010, 30(7)V 0018312 81312 Lg,021544 161536 Lg,001328 3128Lg =,“ 1 1 zL1“b214 k |B k A, Hq “5Q, bTV ,0a00 (sY47216514, 3249765, 18475917; RSDsY112%, 114%,
12、 113%b215 k |B k 6z, k A!ZE! A, HqbTV ,0a00c (sY01070%, 01203%, 01032%; RSDsY213%, 319%, 312%,V E zb216 k |B k A, HqsY0, 4, 8, 12, 16, 24h“, bTV ,0a00 (sY1189737, 7345693, 491193; 8,RSDsY019%, 015%, 019%b217 F“ l q k | = 2 M 3210g, 6z,sY F 0v A1mL,0v A1mL0v A1mL,! k A, Hq,9 l q,TnV1b0a00 ( l qsY9911
13、%, 9810%,9816%;RSDsY319%, 218%, 218%bV1 F“ l q kT(n= 6)Tab 1 Resultsofrecovery testsamples( component)“ ( sample content) /mgF (added) /mg ( found) /mg ( l q( averagerecovery) /%RSD/%0( astragaloside ) 01525 01416 01937 9911 3190 ( astragaloside ) 11782 11272 31028 9810 2180( astragaloside) 01056 01
14、066 01121 9816 218218 “ c | G0,/ 211120/ZE! k Ab Hq0a00 ,TnV2bV2 “ T(%, n= 2)Tab 2 Determination ofsamples“ ( samplecode) (origin)0( astragaloside )0( astragaloside )0(astragaloside)MG-1 (7 3) (Shanx,i growwild) 01047 01121 01014MG-2 (7 3) (Shanx,i growwild) 01066 01141 01024MG-3 (7 3) (Shanx,i grow
15、wild) 01041 01140 01010MG-4 ( ) (Gansu, decoction pieces) 01050 01147 01008MG-5 (7 3) (Hunyuan, Shanx,i growwild) 01055 01162 01013MG-6 (3 M 3) (Shandong, 3 years) 01036 01082 01015MG-7 ( ) (Shanx,i cultivation) 01058 01173 01020MG-8 = (2 M 3) (Neimenggu, 2 years) 01026 01089 01003MG-9 (7 3) (Hunyua
16、n, Shanx,i growwild) 01055 01204 01009MG- 10( )(Hebe,i decoction pieces) 01036 01128 01009MJ-1 a(1 M 3)( Fengning, Chengde, 1 years) 01087 01264 01012MJ-2 a(2 M 3)( Fengning, Chengde, 2 years) 01031 01063 01016MJ-3S(2 M 3) (Anguo, Hebe,i 2 years) 01078 01284 01006MJ-4 Y(3 M 3) (Tonghua, Jinlin, 3 ye
17、ars) 01079 01326 01009( note):MG- 1MG- 10, (A stragalus m em branaceus(Fisch1) Bge1var. m ongholicus (Bge. )Hsiao); MJ- 1MJ- 4, G(A stragalus mem branaceus (Fisch. )Bge. )1193)0sChin JPharm Anal2010, 30(7)3 ) 311 4 |Hq I 40Mq,sY I f4 |a 2 34 |ZE;4 | v d ;J?4 |; iJ? 4 |;i O4 | HW IbTV ,J? f4 |4h4 |r
18、qK,v d“0M q/bNZE T;eL 8 H,7 O q zb312 ELSD I M5 , %Hq/,V50 80 e 5 e1Q,4 .1bT M555e , 8 17214kPaHq/, 30 l. |Mb313 c Ts G(c0a0030s, 7 3WYv, G1 ,00c Yv,7 G0v1012%b314 H030 s,NZEe, VT00 ssZEb00 V0,70 0 T,yN,sKD4 G b ID1 ChP(S0). 2005. Vol(B): 2122 LING Hong- feng( 1 ), SU Dan( ), CAO Yang().Studies on a
19、nti- oxidation capacityofRadixAstragalipolysaccharide( FT). J M ed Theory Pract(D Ll), 2005, 18(8): 8723 WEIX, ZHANG J. A strgalusm ongholius andP olygonum multiflorunp-sp rotective function against cyclophoshamide inhibitory effect onthymns. Am J ChinM ed, 2004, 32(5): 6694 WANG Yao- jun(1 +), QUAN
20、 Q i- zhen( ), SUIZi-qin( 1 W). ICAM- 1 expression in experimental fibrosistissue a-ffectedbyRadixAstragali( L8FICAM - 1VrY fF). Chin J C lin Pharm acol Ther(S “50 ), 2000, 5( 1): 495 ZHAOWen(u), REN Yong- feng( ). Studyon effectsofAstragalusaksuensis against virus( FhT).Chin P harm J (S0), 2001, 36
21、(1): 236 XU Xu(), TANG Li- da( r). Progress in study ofphar-macologicaleffectofAstragalusmembranaceus on cardiovascularsys-tem(50 TZ). Chin J N ew D rugs (S0), 2003, 12( 11): 8997 LIANG Xu-ming( ), TANG Yao- shu(2 ), ZHANGXiao- hai(fl ). Determination of astragaloside in Qihuang cap-sulesbyTLC- scan
22、ning( E 540c ). LishizhenM ed M ater M ed R es( H SDS0), 2007,18( 1): 228 LIXin-xin( ),HAO Gui-ming(yI ), ZHAO Chun-jie(u=), et al. Determination of astragaloside in theRadixAstragalibyRP-HPLC(QMrAM E0c ). Chin Pharm J (S0), 2003, 38(3): 2129 CHENHai-ming(Z ). HPLC-ELSD determination ofastraga-loside inRadixAstragaliand itspreparations(HPLC-ELSDZE# Z40c ). Chin J Pharm A-nal(0s), 2007, 27(3): 426(2009 M1012 l)1194)0sChin JPharm Anal2010, 30(7)
