1、?; yluxABS:A % )NBT)*何琳燕* * null 黄为一( 2 j O4,?;+, 20Q ?;3 F 3 F, a= 3 .1oMnullA % )null?; ynullS:cI|null 1001- 9332( 2004) 07- 1241- 04nullms |null Q93nullDS M null AIntroductionof bioluminescence genes into silicate-dissolving bacteria strainNBT. HE Linyan, HUANG W eiyi(Department of Microbiology,
2、Nanjing Agricultural University, Nanjing 210095, China).-Chin. J. Appl.Ecol. , 2004, 15( 7) : 1241 1244.In this study, silicate- dissolving bacteria NBT strain was grown w ith 0. 25% maltose as carbon source, and the r-ifampicin- resistance w as generated by ultraviolet mutagenesis and streak natura
3、lized to 200nullgnullml- 1. Through tr-iparental cross, the luxAB genes w ere introduced into NBT- R200 at the help of pRK2013. The luminescence ac-tivity of the hybrid strain was tested, w hich indicated that all colonies had a high luminescence activity andkanamycin- , tetracyctine-and rifampicin-
4、 resistance. The NBT- R200 cells prepared from initial logarit hmic growt hphage were more likely to be sensitive to foreign DNA, and the maximum translocation frequency w as up to 6. 70null 10- 5. In addition, the optimal mat ing rat io was 1null1null1. The potassium release ability from feldspar a
5、nd the lu-minescence of lux AB genes marked silicate- dissolving bacteria RL85 strain w ere stable, and hence, the RL85 wasavailable to rhizosphere microecology researches.Key words null Silicate- dissolving bacteria, lux marker genes, Triparental cross.* Z g S/ j1 “( j S39805-2|) .* *Y “ .2003- 07-
6、 28 l, 2004- 02- 12 s.1nullnull nullA % ) r + y 3=%), ?s SA hA F F , a dKa Kas ? 11, 17, 18, 20 , “ -Q4.S: ?;“di15 lux,7S:y 8 |4 1 . ?; yluxAB% kV ,S: )RL85c F 3 | 20Q ?_?;3F 3 F, luxAB% ? i,BW_= 3 3 4 ).12437 null null null null null null null null null null null :?; yluxABS:A % )NBT )null null nul
7、l null null null null null null null nullm4null?; yS: )? )KTFig. 4 Potassium release from feldspar by luxAB genes marked silicate-dissolving bacteria and starting strains.nullnull )Non-inoculated with bacteria, nullnull )Inoculated withdead bacteria, nullnull )Inoculated w ith bacteria.4nullnull nul
8、l 4null1nullh G )8 l“a Y, 0null25% T )8! !.4null2nullYVA % )NBT ),MF 3 FB F 200 nullgnullml- 1NBT-R200 ),c?; ylux AB pTR102nulllux AB pRK2013/ ),V7NBT )?; a 5 * a F 3 F. 3 )8! s8%,?C 3 - % sDNA,M qK Vr6null70 null 10- 5,1 1null1null1 aR.4null3null?; S: )RL85 dK ? ? )NBTM, O20Q F 3 F?;+, aA % )= 3 .
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