1、Qb ID:1 .S S(C ),1997,27(4):334j3402u *.S S(C ),1998,28(1):42j493.% 3,1995,17(2):54j584 . 3 3 , 1998,30(2):147j1535PhiVL et al. J Biol Chem, 1998,273(29):18300j183076Apolonia HM et al. Plant Mol Biol, 1997, 33( 3):553j5577Nabicochikin et al. J Biol Chem, 1998,273(4):2473j24798Manju A et al. J Virol,
2、 1998,72(5):3720j37289Joaquin Cet al. FEBS Let, 1997,400:193j19610Girard F et al. EMBO J, 1998,17:2079j208511Legault Jetal. Biochem Cell Biol, 1997,75(4):369j37512Katrin W et al. ProcNatl Acad Sci USA, 1998,95(23):13681j1368613Deppert W. J Cell Biochem, 1996,62:172j180cI|:1000-1336(1999)05-0225-05AT
3、P :1 Kl?石晓冰, 沈允钢(S S Z 3 , Z200032)1oM:ATP合酶;旋转催化;结合改变机理;质子动力势ms |:Q 559 DS M :AATP h , PATPV db bF1 3 b ATP db,V bnull HW100ms,“Hq/ b HWMb(3) YashidaKinosita L iS B L,A/4 null(m2c) 8b | _ )F1YVy/ nullNF 10His,|null106SerlMCys, H|null CyslMSerb3E.coliVr4BF,null3null3nullYVnull His%V p Ni-NTAj , H|;S
4、:8 YVnull106Cys null ,82.5nullm, F1200, ;A/ V4bMg2+-ATPiHq/,V Z_ A , V AnullT I Hb L L, F1 ATP null3null33b v3bi “nullbF0F1 ,ATP V ? nullQZ_bb4. ATP b4.1 xATP 3 bATP x, ? P9 ,c?b H I H,| %0 V BY b TV/( =)Y , * VF0 A,c Hb =pH0MQ H,_MQZ_b L t 0 wb0 l: nullH+ = - 2.3RTpH+Fnull (F:E , null : ),pH “Y 7M,
5、c = Y02a11b4.3H+ATP / ATP H+ /ATP V,o0pnullnull“1Tbnullnull T , B , null b 7b1b LA U, nullnullanullanullF0c ( VTi228iu 3 v1999 M19 5 bnull ,N 10null|a, ,C null ?Cbnull sT:C null MT,nulla5nullF0cMTbnullATP A, ATP, H 0Yah 0 ?b V ,nullVY;“d db0 u12bATP F0H+ ,nullnull120,BT null dbBATPbATPH+ 8V V ? / Z
6、T13:(1) F0ac, PcAsp61VpKa M pKa ,cV =BH+ , Hnullnullc;(2) ac ,cAsp61pKa ,Asp61MH+ ,H+VpKa Asp61 db , HcnullnullbnullnullF0abdPnullnullvBZ_( H I H)/BpKa cbF0 H+ e wnullnullMnull3null3120,7nullnullYVnull T Pnull(ATP) dbBATP, Hnull(E)null(ATP)T_ op b ID:1WeberJ et al. Biochem Biophys Acta, 1997,1319:19
7、j582Junge W et al. TIBS, 1997,22:420j4233Mitchell P. Physiol Rev, 1966,41:445j502 4Boyer PD. Biochem Biophys Acta, 1993, 140:215j2505Senior AE. Annu Rev Biophys Biophys Cliem, 1990,19:7j416Zhon Y et al. Proc Natl Acad Sci USA, 1997,94:10583j105877Junge W. Photosynthesis: Mechanisms andEffects.1998,1
8、635j1642. GarabG(ed.), KluwerAcademicPublisher, Netherlands.8Noji Het al. Nature, 1997,386:299j3029Abrahams JP et al. Nature, 1994,370:621j628 10Souid AK et al. J Biol Chem, 1995, 270: 9074j908211Fillingame RH. Biochem Biophys Acta, 1998,1365:135j14212 N. 3 ,1998,24(2):119j12313Fillingame RH. J Exp
9、Biol, 1997,200:217j224S Sv S V - 1999 M71 S S? , S S V! V 2217 , V89 ,! V313 b Ss S V /(3 ):* S !46* 75 62 S88?77* 3201*%? 3912026 S58 S98*L.8277 o 95111*DL.aA ?90*D 3 53* 3 f88* * 368 180* 3 0 55* 359*ar 3 S55* 8 3a 3 74* 75 S57o*p14 S 3 MM1, V9 1112 , V 50.2%b1999 M4z9F63 V37 3M1,9 V9 59%b(6K1S= :www4.nas.edu/nashome.nsf/WebLink/members? OpenDocument)i229iu 3 v1999 M19 5
